- Research article
- Open Access
Evolution of Class I cytokine receptors
BMC Evolutionary Biology volume 7, Article number: 120 (2007)
The Class I cytokine receptors have a wide range of actions, including a major role in the development and function of immune and blood cells. However, the evolution of the genes encoding them remains poorly understood. To address this we have used bioinformatics to analyze the Class I receptor repertoire in sea squirt (Ciona intestinalis) and zebrafish (Danio rerio).
Only two Class I receptors were identified in sea squirt, one with homology to the archetypal GP130 receptor, and the other with high conservation with the divergent orphan receptor CLF-3. In contrast, 36 Class I cytokine receptors were present in zebrafish, including representative members for each of the five structural groups found in mammals. This allowed the identification of 27 core receptors belonging to the last common ancestor of teleosts and mammals.
This study suggests that the majority of diversification of this receptor family occurred after the divergence of urochordates and vertebrates approximately 794 million years ago (MYA), but before the divergence of ray-finned from lobe-finned fishes around 476 MYA. Since then, only relatively limited lineage-specific diversification within the different Class I receptor structural groups has occurred.
Cytokines are a class of proteins that includes interleukins (ILs), interferons (IFNs), colony-stimulating factors (CSFs), and tumor necrosis factors (TNFs). These polypeptides are produced and secreted by cells in response to many stimuli and mediate their effects by binding to specific receptors on the surface of target cells [1, 2]. Class I helical cytokines represent the largest group of cytokines and utilize a family of cell-surface receptors that are structurally divergent from those employed by other cytokines, such as the TNF receptor family and receptor tyrosine kinases . The receptors for Class I helical cytokines consist of various receptor chains that associate in higher order homo- and heterotypic complexes. Signaling via these receptors has a myriad of roles, including a major influence on immunity and hematopoiesis [4–6]. There is considerable functional redundancy amongst Class I helical cytokine receptors. This is partially due to some cytokines binding to multiple receptor complexes, multiple cytokines binding to the same receptor complex, and the sharing of common signal transducing receptor chains – and so downstream signaling pathways – by different receptor complexes .
Class I helical cytokine receptors share little primary sequence homology . Although individual Class I helical cytokine receptor chains vary in overall topology, they all posses a conserved 200 amino acid extracellular region that is required for ligand-receptor interactions . This is known variously as the cytokine receptor homology domain (CHD), or the D200 [8, 10]. The CHD consists of two tandem fibronectin type III (FBN) folds and contain distinctive elements that distinguish Class I from the Class II family . The Class I receptor CHD contains two pairs of conserved cysteines linked via disulfide bonds and arranged in a CX-(9–10)-CXWX-(26–32)-CX-(10–15)-C motif within the first FBN fold. The second FBN fold contains a highly conserved, although slightly variable such as in GHR, WSXWS motif at its C-terminus [8, 11]. In addition to the CHD, Class I cytokine receptor chains consist of a range of other modules, including extracellular immunoglobulin (Ig)-like and FBN domains, a transmembrane domain, and conserved intracellular motifs, including Box 1 and Box 2 motifs that are associated with Janus Kinase (Jak) docking [1, 2, 8].
Class I cytokine receptor complexes have traditionally been divided into families based on the use of common signal transducing chains within a receptor complex . However, more recently researchers have placed the individual receptor chains into five groups. This is based on sequence and structural homology of the receptor and its cytokine ligand (Figure 1), which is potentially more reflective of evolutionary relationships . Group 1 receptor chains have an extracellular domain that consists solely of a CHD. This group contains the erythropoietin receptor (EPOR), thrombopoietin receptor (TPOR), prolactin receptor (PRLR), and growth hormone receptor (GHR) chains that each form homodimers in the presence of their respective ligands [10, 12], as well as an orphan receptor, CLF-3, of unknown function . Group 2 receptors are the most numerous and are structurally related to the archetypal glycoprotein 130 (GP130). Typically, Group 2 receptors chains have an N-terminal Ig-domain and FBN modules between their CHD and transmembrane domains [10, 13]. Group 3 receptor chains also generally possess an N-terminal Ig domain in addition to the CHD, and are either soluble or have short intracellular regions . Receptor chains from Groups 2 and 3 collectively constitute the large IL-6R family of receptor complexes that often share GP130 as a common signal transducer . Group 4 receptors typically consist solely of an extracellular CHD domain and long intracellular domains, whereas Group 5 receptors often possess extracellular Ig domains in addition to the CHD, and have short intracellular regions . Group 4 and 5 receptor chains associate to form receptor complexes of the IL-2R and IL-3R families, with IL-2Rγc and IL-3Rβc being the shared chains respectively [3, 7, 10, 11].
The Class I cytokine receptors are collectively responsible for a great diversity of cytokine-mediated responses. To further our understanding of the evolution of this complex signaling system, we have explored the repertoire of Class I receptor chains in sea squirt and zebrafish. This has revealed a period of massive expansion of Class I receptors between the divergence of urochordates and vertebrates around 794 million years ago (MYA), and divergence of ray-finned and lobe-finned fishes some 476 MYA. In contrast, more moderate lineage-specific expansion of these receptors has occurred since that time.
Identification of putative sea squirt Class I cytokine receptor chains
Exhaustive analysis of sea squirt genomic databases revealed the presence of just two genes encoding putative Class I cytokine receptor chains. The presence of corresponding ESTs verified expression of both genes (Table 1). One of these showed broad sequence homology and conserved topology, including signature CHD motifs, with the archetypal GP130 of vertebrates and was designated cigp130-like (Figure 2a). The other receptor chain showed high conservation with sequences available for the orphan receptor, CLF-3, which essentially consists of just a CHD domain (Figure 2b). Phylogenetic analysis of the respective CHDs confirmed these designations, and revealed that each was equally divergent from the dome receptor sequences from Drosophila melanogaster (Figure 2c), despite the topological similarity between the dome sequences and cigp130-like. Furthermore, the ciclf-3 is encoded by a single exon, like the CHD of dmdome proteins (Figure 2d). In contrast, cigp130-like has a complex splice structure resembling that of GP130, that is largely conserved in other Class I receptors, with the exception of the vertebrate CLF-3 sequences that have a completely different splice pattern. No CLF-3-related sequences were found in D. melanogaster or Anopheles gambiae, suggesting that ciclf-3 was derived from at least a partial duplication of an ancestral dome-like sequence. There were no conserved syntenic relationships between either D. melanogaster or C. intestinalis cytokine receptors and their vertebrate counterparts (Additional file 1).
Identification of zebrafish genes encoding class I cytokine receptor chains
A total of 36 genes encoding putative Class I cytokine receptors chains were identified in zebrafish, including at least one representative from each structural group (Table 1, Figures 3 and 4). To both confirm that these genes were transcribed, as well as to commence their functional characterization, RT-PCR was performed on total RNA extracted from whole zebrafish embryos 24 hpf and 72 hpf using primers specific for each. This yielded appropriately sized products at one or both time points for all but three contigs. The presence of an EST corresponding to the putative gene provided alternate confirmation of expression for two of these, dril-3rβc and dril-12rβ2.b. The presence of equivalent open reading frames in Takifugu rubripes or T. nigroviridis for the majority of zebrafish receptors provided additional support for this assertion (data not shown). Combined with the presence of long open reading frames in the contigs, this represented compelling evidence that the vast majority of contigs identified were coding genes and not pseudogenes. Only dril-21rα.b lacked any supporting evidence of expression, although a T. nigroviridis orthologue was present (data not shown).
Each of the Group 1 receptor chains found in mammals was represented at least once in zebrafish as determined by sequence, topology, and synteny conservation (Table 1), and confirmed by phylogenetic analysis (Figure 3a, Additional file 4). There were single zebrafish orthologues for CLF-3 EPOR, and TPOR. In contrast, the GHR and PRLR subfamily was expanded in zebrafish, with two clear homologues for PRLR (prlr.a, prlr.b), a single GHR, and an additional somatolactin receptor (slr) (Additional file 2). Furthermore an additional receptor was identified as belonging to the GHR and PRLR subfamily. Phylogenic analysis of the CHD domain grouped this receptor with GHR, it also has the presence of a typical WSXWS motif seen in the CHD of PRLR, but not GHR and SLR. Due to the lack of comparable tetrapod sequences it was named after the T. nigroviridis receptor, CRFA4, and is possibly a teleost specific receptor.
Of the ten mammalian Group 2 receptors, potential orthologues for eight were found in zebrafish (Table 1). This included one for the archetypal member of the group, GP130, which is the common shared component of many IL-6R family receptors. Interestingly, the zebrafish gp130 sequences did not form a distinct clade with the tetrapod GP130 sequences using CHD only strategies (Figure 3b, Additional file 5). However, multiple sequence alignment of the full-length protein revealed areas of significant homology throughout the protein that continued into functionally important areas of the intracellular domain, including Box 1 and Box 2 (Jak docking), a serine rich region, Box 3, a STQPLLDXEEX internalization motif, and tyrosine residues essential for docking of Stat3 (YXXQ) and SHP-2/SOCS3 (YXXV) [14–16] (Figure 5a). A zebrafish orthologue was also found for the closely-related GCSFR, which has the same overall topology to GP130 . Synteny analysis suggested that there was no conserved relationship between humans and zebrafish (Additional file 3). However, there was conserved synteny between human and T. rubripes, thus providing further evidence that the zebrafish sequence is indeed a GCSFR orthologue. Mammalian IL-12Rβ2 also exhibits the same topology as GP130 and is syntenic to IL-23R with which it also shows high homology , although the latter lacks the FBN domains . The zebrafish orthologues also maintain conserved synteny (Additional file 3), although zebrafish il-23r possesses FBN domains as does IL-12Rβ2, but lacks a transmembrane domain.
Several Group 2 receptors have a duplication of the CHD, partially in OSMR, fully in the closely related LIFR, and including a duplicated N-terminal Ig domain in the more divergent OBR . Zebrafish have three related receptors that show similar sequence identity to both OSMR and LIFR. All sequences showed topological similarity to OSMR, having only a partial duplication of the N-terminal CHD, termed "CHD 0.5". Two of these sequences, lifr.a and lifr.b, are grouped with LIFR on the basis of phylogenic analysis of the CHD. One, lifr.a, showed extensive conservation of intracellular homology motifs with LIFR, including Box 1, Box 2, Box 3, multiple Stat3 binding sites and a C-terminal GSPXIXSXQFLIP internalization motif, absent in drlifr.b and OSMR  (Figure 5b). Although the intracellular region of drosmr was unable to be determined, the extracellular region showed strong sequence and synteny conservation with OSMR (Additional file 3). In contrast, a clear OBR homologue was identified in zebrafish, drobr, which maintains the same topology as mammalian OBR. No zebrafish homologues were found for the remaining Group 2 receptors – IL-12Rβ1, IL-27Rα, and GLMR – with their unique topology apparently specific to tetrapods.
Zebrafish homologues were found for all six mammalian members of Group 3 (Table 1). Single fish orthologues were identified for IL-27Rβ, IL-6Rα, CNTFRα, and IL-11Rα, while zebrafish possessed two homologues for both CLF-1 (drclf-1.a and drclf-1.b) and IL-12p40 (dril-12p40.a and dril-12p40.b). In each case, there was conserved topology and clades formed with high bootstrap values (Figure 3c, Additional file 6).
Mammalian Group 4 consists of six receptor chains in humans and seven in mice, the latter with an additional copy of IL-3Rβc . No zebrafish orthologues were found for either the additional IL-3Rβc or for IL-9Rα, but each of the other five members was represented (Figure 3d, Additional file 7), albeit with weak homology. The identity of these marginal orthologues was confirmed by conserved topology for IL-3Rβc (Figure 6), conserved synteny for IL-2Rβ, IL-4Rα, and IL-7Rα (Additional file 3). Further evidence for the IL-7Rα orthologues was aided by the conserved intracellular motifs, including Box 1 and a Stat5 docking site (Figure 5c) . In contrast, there were two paralogues of IL-21Rα found, although neither displayed conserved synteny with human IL-21Rα.
Finally only four of the seven mammalian Group 5 receptor chains were represented in zebrafish (Figure 4, Additional file 8). A single orthologue was identified for IL-13Rα2 and two zebrafish sequences were found that were most closely related to IL-2Rγc, dril-2γc.a, and dril-2γc.b. Conserved synteny provided evidence for the identification of the zebrafish orthologues of TSLPR and IL-13Rα1, with the latter displaying only limited sequence homology. Additionally both the zebrafish tslpr identified and the second isoform of human TSLPR (Genbank accession no. NP_001012288) lack the first half of the CHD. No zebrafish orthologues were found for IL-3Rα, GMCSFRα, and IL-5R.
The aim of this study was to further our understanding of the evolution of class I cytokine signaling. Since Class I cytokines share little primary sequence , analysis of their receptors provides the best means to of achieving this aim. We therefore employed bioinformatic approaches to characterize the Class I cytokine receptor repertoire within the sea squirt and zebrafish genomes. However, the divergence of class I cytokine receptors, means that phylogenetic trees and alignments are sometimes unreliable. Therefore, the identification of receptor sequences was additionally guided by overall receptor topology and, in particular, conservation of synteny. This robust methodology successfully identified two representatives in sea squirt and 36 representatives in zebrafish. Comparison with the equivalent receptors in insects and mammals has yielded considerable insight into the evolution of this important family of receptors.
Sea squirt possess a classical Class I receptor with the signature Ig-CHD-FBN-TM-Box 1 topology found in D. melanogaster dome and vertebrate GP130 proteins (including that of zebrafish). The presence of a single Class I receptor related to dome/GP130 in Anopheles gambiae confirmed that an archetypal Class I receptor of similar topology existed at 974 MYA, when protostomes and deuterostomes diverged, and from which all other receptors were derived (Figure 6). This was supported by the splicing pattern of cigp130-like that was largely conserved in all vertebrate Class I receptors, except CLF-3. In higher vertebrates, GP130 functions as part of heteromeric receptor complexes. However, the absence of other compatible receptor chains suggests that cigp130-like signals in a homodimeric manner, presumably similar to the dome receptors of D. melanogaster.
The second sea squirt receptor showed unequivocal homology to CLF-3 and, like the CHD of dome, was the product of a single coding exon. This suggests that the CLF-3 precursor arose from a duplication of at least the CHD of an archetypal dome-like receptor, and that it subsequently developed an independent splicing pattern in the evolution of vertebrate CLF-3 genes. The high conservation of CLF-3 despite its ancient origins are indicative of an essential conserved function for this protein. However, despite the presence of a WSXWS motif, CLF-3 proteins lack the signature CHD CX-(9–10)-CXWX-(26–32)-CX-(10–15)-C motif, and possibly a leader sequence, indicating an altered function compared to other class I cytokine receptors. This suggests that CLF-3 may be best considered as a unique protein despite its shared evolutionary origins to the other class I cytokine receptors. This has some similarities to Tissue Factor, which has evolved a divergent biochemical function in blood coagulation despite sharing origins with the class II cytokine receptors .
Therefore, by the time of the last common ancestor of vertebrates and urochordates 794 MYA, two different receptor topologies (and the WSXWS motif) had been generated, only one of which would ultimately generate the great diversity of Class I cytokine receptor chains seen in higher vertebrates, although the exact details remain elusive (Figure 2c).
Comparison of the zebrafish and mammalian Class I cytokine receptor repertoires suggest that between 794 MYA and 476 MYA there was a considerable expansion of both the number of receptor topologies (combinations of structural subdomains) as well as the total number of receptor chains. The result was eight different topologies for the 27 core receptor chains that were likely to be present in the common ancestor of fish and mammals (Figure 6). During this period it has been hypothesized that two whole genome duplications have occurred . These events would explain some of the increase in the diversity of receptor chains. However, only one CLF-3-like receptor is present in both humans and zebrafish. Thus the two whole genome duplications alone theoretically account for only four GP130-related receptors. Therefore, other processes, such as tandem and en bloc duplication have likely driven this expansion.
Since their last common ancestor, the number of cytokine receptor chains have increased to 36 receptors in zebrafish and 36 in humans, although the cause of this expansion is likely different in each lineage. The teleost lineage is believed to have experienced a further whole genome duplication event , which is probably responsible for several novel teleosts receptors, such as the paralogues of the ghr, prlr, lifr, il-12rβ2, clf-1, il-12p40, and il-2rγc. However, evidence of en bloc duplications is also apparent in the il-21rα paralogues, as well as crfa4. In contrast, receptor repertoire expansion within mammals appears primarily to be the result of tandem or en bloc duplications. This is typified in the generation of GLMR, IL-12Rβ1 and IL-27Rα within Group 2, and of the Group 5 receptors that lie relatively adjacent on chromosome X . Similar duplication apparently explain the ongoing cytokine receptor (and ligand) evolution in D. melanogaster .
Some Class I receptor chains have maintained a one:one (1:1) homologue relationship between mammals and teleosts, suggesting conserved functions. For example, a single GP130 orthologue was also present in teleosts with conserved intracellular Jak and Stat3 docking motifs [14–16]. Moreover, many of the Group 3 receptor chains that form complexes with GP130 also largely showed a 1:1 homologue relationship. Other orthologous 1:1 receptor genes found were EPOR, CLF-3, GCSFR, IL-23Rα, OBR, CNTFRα, IL-6Rα, IL-11Rα, IL-27Rβ, IL-7Rα, IL-3Rβc, IL-13Rα1, IL-13Rα2, and TSLPR. Many of these also showed the highest percent similarity and conserved topology, further attesting to their likely conserved roles. An obvious exception was IL-23Rα, which showed different topology in teleosts and tetrapods, lacking a transmembrane domain in the former and FBN repeats in the latter. However, conserved synteny and sequence within the CHD strongly suggest that the zebrafish sequence is an il-23rα orthologue.
In contrast, lineage-specific expansion was seen in both teleosts and mammals probably representing a diversification of function. In support of this, expansion of Group 1 receptor chains within teleosts that has produced multiple paralogues for the PRLR/GHR subgroup. Prolactin signaling in fish plays a drastically different function with diverse roles such as pigment cell function and osmoregulation [23, 24], contrasting to its role in mammary gland development and lactation in mammals . It remains speculative as to whether prolactin binds to prlr.a or prlr.b, to both prlr.a and prlr.b, or whether there may be more than one prolactin. Fish also have a unique cytokine related to both growth hormone and prolactin, somatolactin, which has roles in background adaptation, stress response and acid-base regulation . Indeed zebrafish has recently been shown to have two somatolactins, slα and slβ, a growth hormone, and a prolactin . Zebrafish has a clear orthologue of salmonid slr, although whether slα or slβ, or both bind to zebrafish slr needs to be established. The zebrafish ghr is less problematic, clearly orthologues to the single ghr in salmonids (see additional file 2) [28, 29]. The remaining more divergent homologue of PRLR/GHR, crfa4, also does not have a direct mammalian orthologue, and is most closely related sequence to the orphan T. nigroviridis receptor CRFA4. Other receptor genes expansions are limited to Group 3 (IL-12p40 and CLF-1) and Group 4 (IL-21Rα), although the duplication of CLF-1 appears to be specific for zebrafish rather than all teleosts, as they are absent in pufferfish (data not shown).
Mammalian lineage-specific expansion has occurred in other structural groups. Within Group 2 the IL-12Rβ1, IL-27Rα, and GLMR receptor chains are unique to tetrapods. These receptors are topologically similar lacking an N-terminal Ig-domain – a unique topology – and so are likely derived from the same ancestral receptor. IL-12Rβ1 a member of the IL-12R subfamily is a shared component of both IL-12R and IL-23R complexes promoting Th1 cell and memory T cell development respectively . IL-27R is also involved in the regulation of Th1 cell differentiation . It remains to be seen whether these higher order immune functions are conserved in fish . In contrast, IL-9Rα likely arose from a duplication of IL-2Rβ. In mammals, IL-9Rα has been implicated in asthma and immune responses against parasites , the former property clearly not relevant in an aquatic environment. Lineage-specific expansion was most evident in Group 5 with no teleost homologues found for several mammalian receptors. Interestingly, this is largely limited to receptor chains that form complexes of the IL-3R functional family. Specifically, GMCSFRα, IL-3Rα, and IL-5Rα could have been formed via multiple rounds of duplication of either IL-13Rα1, IL-13Rα2, or TSLPR on chromosome X. The IL-3R functional family is involved in the development of eosinophils, granulocytes, macrophages, and monocytes. Of these, IL-5 signaling plays a specific role in eosinopoiesis . Interestingly, teleosts have no clear analogue for this cell-type , and we have been unable to identify orthologues of a range of eosinophil-specific genes in zebrafish (data no shown). Thus, the IL-5R may have played a direct role in the evolutionary ontogeny of this cell type. The expansion of the IL-3R family has continued within mammalian lineages as IL-3Rβc has been duplicated to generate, IL-3Rβc and IL-3RβIL-3 in mouse , that latter of which is a pseudogene in humans.
Innate immunity, including complement and Toll receptors are well developed in invertebrates, as characterized by D. melanogaster [36, 37]. In this organism, the domeless receptors play diverse roles in oogenesis, eye and gut development, with a more minor role in the D. melanogaster immune system. Adaptive immunity, on the other hand, arose after sea squirt . Our data suggest that a massive increase in the number of Class I cytokine receptors correlated with the development of acquired immunity and the refinement of innate immunity. This suggests that the expansion and subsequent specialization of dome/gp130-like receptors may have played a major role in the evolution of the immune system. In contrast, chemokines and their receptors, which are not present in sea squirt , probably only contributed at later stages of immune system evolution. Further data mining or functional studies of species between urochordates and teleosts, such as lampreys and hagfish, is required for additional insight into this interesting family of receptors, while it is anticipated that reverse genetics approaches in zebrafish may shed light on the function of CLF-3 receptors.
Data mining and sequence assembly
Searches were performed using Class I cytokine receptor sequences and a Class I CHD consensus motif . These sequences were used to systematically interrogate the sea squirt genomic  and Expressed Sequence Tag (EST) databases  as well as the zebrafish EST, genomic, and whole genome shotgun (WGS) databases , using tBLASTn. All independent sequences possessing E values > 0.1 were extracted for further analysis. GenomeScan  was used to predict coding exons from sequences derived solely from WGS or genomic scaffolds, some of which were manually adjusted on the basis of known intron-exon boundaries in other organisms. Nucleotide sequences were assembled using Sequencher 4.1.4 (Gene Codes Corporation). Any apparently incomplete contigs were extended by iterative BLASTn searches using the relevant contig terminus until the entire putative coding sequence had been identified, with any remaining gaps closed by sequencing of appropriate reverse transcription-polymerase chain reaction (RT-PCR) product. The position of intron/exon boundaries was determined by alignment of cDNA and genomic sequences, applying the GT-AG splice rule where possible .
Reverse Transcription-Polymerase Chain Reaction
Total RNA was extracted from zebrafish embryos at 24 and 72 hours post fertilization (hpf). This was converted into cDNA using oligo-dT primers (Roche) and reverse-transcriptase (Invitrogen), which was used as a template for PCR with Taq polymerase (Invitrogen) in an iCycler thermocycler (Biorad) and oligonucleotides designed to span at least one intron to eliminate the potential for amplifying genomic sequences. Negative control templates were water and samples in which the reverse transcriptase was omitted. If required for contig assembly, RT-PCR products were cloned into pGEM-T EASY (Promega) and subsequently sequenced using Big Dye Terminator (Applied Biosystems).
Sequence analysis and nomenclature
The probable identity of each encoded receptor chain was determined by pBLAST searching with the respective conceptual translations. The initial BLAST search was followed by multiple sequence alignments using AlignX 9 (Invitrogen) and ClustalX 1.83 . The latter were used to create bootstrapped phylogenetic tree of 1000 replicates with the Neighbor-Joining algorithm, formatted using njplot , and viewed in Treeview 1.6.6 . Additional analysis using maximum parsimony  and maximum likelihood  algorithms was performed with phylo_win  and phylip  packages to confirm phylogenetic topologies.
Synteny analysis was performed on the putative receptor chains to further access the identity of these receptor chains. Ensembl  was used to perform synteny analysis using version 2 of the C. intestinalis (JGI 2) assembly and version 6 of the D. rerio (Zv6) assembly. Synteny data from either C. intestinalis or D. rerio was primarily compared to that of humans (NCBI 36). However, in certain cases Tetraodon nigroviridis (TETRAODON 7), Takifugu rubripes (FUGU 4.0), Xenopus tropicalis (JGI 4.1), Gallus gallus (WASHUC 1), and Mus musculus (NCBI m36) were also used in the synteny comparison.
The final assignment of identity was guided by conservation of topology, synteny, and overall sequence such as the conservation of functional domains, including intracellular sequences that mediate interactions with the key signal transduction pathway used by cytokine receptors, the Jak-Stat (Signal transducer and activator of transcription) pathway . The nomenclature for the zebrafish genes followed the conventions of zebrafish information network (ZFIN) . Sea squirt genes were named using similar criteria. All sequences were subsequently deposited in GenBank (Table 1) except zfprlr.a (GenBank accession no. AY375318).
Basic local alignment search tool
Cytokine receptor homology domain
Cytokine receptor like factor
Ciliary neurotrophic factor
Cytokine receptor family Class I
Expressed sequence tag
GP130-like monocyte receptor
Hours post fertilization
Leukemia inhibitory factor
Million years ago
Signal transducers and activators of transcription
Tumor necrosis factor
Thymic stromal lymphopoietin
Whole genome shotgun
Ihle JN: Cytokine receptor signalling. Nature. 1995, 377 (6550): 591-594. 10.1038/377591a0.
Kishimoto T, Taga T, Akira S: Cytokine signal transduction. Cell. 1994, 76: 253-262. 10.1016/0092-8674(94)90333-6.
Sato N, Miyajima A: Multimeric cytokine receptors: common versus specific functions. Curr Opin Cell Biol. 1994, 6 (2): 174-179. 10.1016/0955-0674(94)90133-3.
Richmond TD, Chohan M, Barber DL: Turning cells red: signal transduction mediated by erythropoietin. Trends Cell Biol. 2005, 15 (3): 146-155. 10.1016/j.tcb.2005.01.007.
Taga T: Gp130, a shared signal transducing receptor component for hematopoietic and neuropoietic cytokines. J Neurochem. 1996, 67 (1): 1-10.
Kang J, Der SD: Cytokine functions in the formative stages of a lymphocyte's life. Curr Opin Immunol. 2004, 16 (2): 180-190. 10.1016/j.coi.2004.02.002.
Ozaki K, Leonard WJ: Cytokine and cytokine receptor pleiotropy and redundancy. J Biol Chem. 2002, 277 (33): 29355-29358. 10.1074/jbc.R200003200.
Bazan JF: Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA. 1990, 87 (18): 6934-6938. 10.1073/pnas.87.18.6934.
Bravo J, Heath JK: Receptor recognition by gp130 cytokines. EMBO J. 2000, 19 (11): 2399-2411. 10.1093/emboj/19.11.2399.
Boulay JL, O'Shea JJ, Paul WE: Molecular phylogeny within type I cytokines and their cognate receptors. Immunity. 2003, 19 (2): 159-163. 10.1016/S1074-7613(03)00211-5.
Bagley CJ, Woodcock JM, Stomski FC, Lopez AF: The structural and functional basis of cytokine receptor activation: lessons from the common beta subunit of the granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 receptors. Blood. 1997, 89 (5): 1471-1482.
Heldin CH: Dimerization of cell surface receptors in signal transduction. Cell. 1995, 80 (2): 213-223. 10.1016/0092-8674(95)90404-2.
Taga T, Kishimoto T: GP130 and the interleukin-6 family of cytokines. Annu Rev Immunol. 1997, 15: 797-819. 10.1146/annurev.immunol.15.1.797.
Murakami M, Narazaki M, Hibi M, Yawata H, Yasukawa K, Hamaguchi M, Taga T, Kishimoto T: Critical cytoplasmic region of the interleukin 6 signal transducer gp130 is conserved in the cytokine receptor family. Proc Natl Acad Sci USA. 1991, 88 (24): 11349-11353. 10.1073/pnas.88.24.11349.
Dittrich E, Rose-John S, Gerhartz C, Mullberg J, Stoyan T, Yasukawa K, Heinrich PC, Graeve L: Identification of a region within the cytoplasmic domain of the interleukin-6 (IL-6) signal transducer gp130 important for ligand-induced endocytosis of the IL-6 receptor. J Biol Chem. 1994, 269 (29): 19014-19020.
Narazaki M, Witthuhn BA, Yoshida K, Silvennoinen O, Yasukawa K, Ihle JN, Kishimoto T, Taga T: Activation of JAK2 kinase mediated by the interleukin 6 signal transducer GP130. PNAS. 1994, 91 (6): 2285-2289. 10.1073/pnas.91.6.2285.
Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O'Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW: A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rb1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002, 168 (11): 5699-5708.
Thiel S, Behrmann I, Timmermann A, Dahmen H, Muller-Newen G, Schaper F, Tavernier J, Pitard V, Heinrich PC, Graeve L: Identification of a Leu-Ile internalization motif within the cytoplasmic domain of the leukaemia inhibitory factor receptor. Biochem J. 1999, 339: 15-19. 10.1042/0264-6021:3390015.
Jiang Q, Li WQ, Hofmeister RR, Young HA, Hodge DR, Keller JR, Khaled AR, Durum SK: Distinct regions of the interleukin-7 receptor regulate different Bcl2 family members. Mol Cell Biol. 2004, 24 (14): 6501-6513. 10.1128/MCB.24.14.6501-6513.2004.
Renauld JC: Class II cytokine receptors and their ligands: key antiviral and inflammatory modulators. Nat Rev Immunol. 2003, 3 (8): 667-676. 10.1038/nri1153.
Dehal P, Boore JL: Two rounds of whole genome duplication in the ancestral vertebrate. PLoS Biol. 2005, 3 (10): e314-10.1371/journal.pbio.0030314.
Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, Bouneau L, Fischer C, Ozouf-Costaz C, Bernot A, Nicaud S, Jaffe D, Fisher S, Lutfalla G, Dossat C, Segurens B, Dasilva C, Salanoubat M, Levy M, Boudet N, Castellano S, Anthouard V, Jubin C, Castelli V, Katinka M, Vacherie B, Biemont C, Skalli Z, Cattolico L, Poulain J, De Berardinis V, Cruaud C, Duprat S, Brottier P, Coutanceau JP, Gouzy J, Parra G, Lardier G, Chapple C, McKernan KJ, McEwan P, Bosak S, Kellis M, Volff JN, Guigo R, Zody MC, Mesirov J, Lindblad-Toh K, Birren B, Nusbaum C, Kahn D, Robinson-Rechavi M, Laudet V, Schachter V, Quetier F, Saurin W, Scarpelli C, Wincker P, Lander ES, Weissenbach J, Roest Crollius H: Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype. Nature. 2004, 431 (7011): 946-957. 10.1038/nature03025.
Power DM: Developmental ontogeny of prolactin and its receptor in fish. Gen Comp Endocrinol. 2005, 142 (1-2): 25-33. 10.1016/j.ygcen.2004.10.003.
Oshima N, Goto M: Prolactin signaling in erythrophores and xanthophores of teleost fish. Pigment Cell Res. 2000, 13 Suppl 8: 35-40. 10.1034/j.1600-0749.13.s8.8.x.
Bole-Feysot C, Goffin V, Edery M, Binart N, Kelly PA: Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev. 1998, 19 (3): 225-268. 10.1210/er.19.3.225.
Huising MO, Kruiswijk CP, Flik G: Phylogeny and evolution of class-I helical cytokines. J Endocrinol. 2006, 189 (1): 1-25. 10.1677/joe.1.06591.
Zhu Y, Song D, Tran NT, Nguyen N: The effects of the members of growth hormone family knockdown in zebrafish development. Gen Comp Endocrinol. 2007, 150 (3): 395-404. 10.1016/j.ygcen.2006.10.009.
Fukada H, Ozaki Y, Pierce AL, Adachi S, Yamauchi K, Hara A, Swanson P, Dickhoff WW: Salmon growth hormone receptor: molecular cloning, ligand specificity, and response to fasting. Gen Comp Endocrinol. 2004, 139 (1): 61-71. 10.1016/j.ygcen.2004.07.001.
Fukada H, Ozaki Y, Pierce AL, Adachi S, Yamauchi K, Hara A, Swanson P, Dickhoff WW: Identification of the salmon somatolactin receptor, a new member of the cytokine receptor family. Endocrinology. 2005, 146 (5): 2354-2361. 10.1210/en.2004-1578.
Watford WT, Hissong BD, Bream JH, Kanno Y, Muul L, O'Shea JJ: Signaling by IL-12 and IL-23 and the immunoregulatory roles of STAT4. Immunol Rev. 2004, 202: 139-156. 10.1111/j.0105-2896.2004.00211.x.
Owaki T, Asakawa M, Morishima N, Hata K, Fukai F, Matsui M, Mizuguchi J, Yoshimoto T: A role for IL-27 in early regulation of Th1 differentiation. J Immunol. 2005, 175 (4): 2191-2200.
Trede NS, Langenau DM, Traver D, Look AT, Zon LI: The use of zebrafish to understand immunity. Immunity. 2004, 20: 367-379. 10.1016/S1074-7613(04)00084-6.
Demoulin JB, Van Snick J, Renauld JC: Interleukin-9 (IL-9) induces cell growth arrest associated with sustained signal transducer and activator of transcription activation in lymphoma cells overexpressing the IL-9 receptor. Cell Growth Differ. 2001, 12 (3): 169-174.
Warren DJ, Moore MA: Synergism among interleukin 1, interleukin 3, and interleukin 5 in the production of eosinophils from primitive hemopoietic stem cells. J Immunol. 1988, 140: 94-99.
Bennett CM, Kanki JP, Rhodes J, Liu TX, Paw BH, Kieran MW, Langenau DM, Delahaye-Brown A, Zon LI, Fleming MD, Look AT: Myelopoiesis in the zebrafish, Danio rerio. Blood. 2001, 98 (3): 643-651. 10.1182/blood.V98.3.643.
Imler JL, Zheng L: Biology of Toll receptors: lessons from insects and mammals. J Leuk Biol. 2004, 75: 18-26. 10.1189/jlb.0403160.
Nonaka M, Yoshizaki F: Primitive complement system of invertebrates. Immunol Rev. 2004, 198: 203-215. 10.1111/j.0105-2896.2004.00118.x.
Klein J, Nikolaidis N: The descent of the antibody-based immune system by gradual evolution. Proc Natl Acad Sci USA. 2005, 102 (1): 169-174. 10.1073/pnas.0408480102.
DeVries ME, Kelvin AA, Xu L, Ran L, Robinson J, Kelvin DJ: Defining the origins and evolution of the chemokine/chemokine receptor system. J Immunol. 2005, 176 (1): 401-415.
JGI C. intestinalis v1.0 Home. [http://genome.jgi-psf.org/ciona4/ciona4.home.html]
BLAST Zebrafish Sequences. [http://www.ncbi.nlm.nih.gov/genome/seq/BlastGen/BlastGen.cgi?taxid=7955]
Padgett RA, Grabowski PJ, Konarska MM, Seiler S, Sharp PA: Splicing of messanger RNA precursors. Annu Rev Biochem. 1986, 55: 1119-1150. 10.1146/annurev.bi.55.070186.005351.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res. 1997, 25 (24): 4876-4882. 10.1093/nar/25.24.4876.
Barnabas J, Goodman M, Moore GW: Descent of mammalian alpha globin chain sequences investigated by the maximum parsimony method. J Mol Biol. 1972, 69: 249-278. 10.1016/0022-2836(72)90229-X.
Felsenstein J: Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981, 17: 368-376. 10.1007/BF01734359.
Ward AC, Touw I, Yoshimura A: The Jak-Stat pathway in normal and perturbed hematopoiesis. Blood. 2000, 95 (1): 19-29.
Zebrafish Nomenclature Guidelines. [http://zfin.org/zf_info/nomen.html]
Leonard WJ, Lin JX: Cytokine receptor signaling pathways. J Allergy Clin Immunol. 2000, 105 (5): 877-888. 10.1067/mai.2000.106899.
Blair JE, Hedges SB: Molecular phylogeny and divergence times of deuterostome animals. Mol Biol Evol. 2005, 22 (11): 2275-2284. 10.1093/molbev/msi225.
CL was supported by a Deakin University Postgraduate Research Award. The authors would like to thank Lynda O'Sullivan, James Burrows, and Rowena Lewis for their helpful discussions regarding the manuscript.
CL performed the bulk of the data mining, phylogenetic analysis, and expression studies, as well as drafting the manuscript. ACW conceived the project, participated in its design and analysis and helped to draft the manuscript. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: C. intestinalis and D. melanogaster cytokine receptor sequences. Additional file is a pdf document that contains supplementary data about the synteny analysis performed in C. intestinalis and D. melanogaster. Grey boxes represents genes in humans, and grey outline boxes represent genes in zebrafish. (PDF 591 KB)
Additional file 2: . Additional file is a pdf document that contains supplementary data about the phylogenetic analysis performed for the gh, prl, and sl receptor family. The phylogenetic tree was calculated using full-length gh, prlr, and sl receptor sequences from zebrafish along with those from human (hs), mouse (mm), T. nigroviridis (tn), and Oryzias latipes (ol). Additionally mammalian CLF-3 and EPOR were used as outgroups. (PDF 463 KB)
Additional file 3: . Additional file is a pdf document that contains supplementary data about the synteny analysis performed primarily comparing zebrafishand humans. Additionally T. nigroviridis, T. rubripes, X. Tropicalis, G. gallus, and M. musculus were also used in the synteny comparison where required. Grey boxes represents genes in humans, and grey outline boxes represent genes in zebrafish. The partial sequences (tpor), and a sequence with a missense mutation (il-4r) have also been included. (PDF 2 MB)
Additional file 4: . Additional file is a pdf document that contains supplementary data. Included is an alignment of group 1 Class I cytokine receptors that were used to calculate the phylogenetic tree in Figure 3a. (PDF 13 KB)
Additional file 5: . Additional file is a pdf document that contains supplementary data. Included is an alignment of group 2 Class I cytokine receptors that were used to calculate the phylogenetic tree in Figure 3b. (PDF 16 KB)
Additional file 6: . Additional file is a pdf document that contains supplementary data. Included is an alignment of group 3 Class I cytokine receptors that were used to calculate the phylogenetic tree in Figure 3c. (PDF 12 KB)
Additional file 7: . Additional file is a pdf document that contains supplementary data. Included is an alignment of group 4 Class I cytokine receptors that were used to calculate the phylogenetic tree in Figure 3d. (PDF 13 KB)
Authors’ original submitted files for images
About this article
Cite this article
Liongue, C., Ward, A.C. Evolution of Class I cytokine receptors. BMC Evol Biol 7, 120 (2007). https://doi.org/10.1186/1471-2148-7-120
- Cytokine Receptor
- Growth Hormone Receptor
- Whole Genome Shotgun
- Receptor Chain
- Zebrafish Orthologues