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The homology of odontodes in gnathostomes: insights from Dlx gene expression in the dogfish, Scyliorhinus canicula



Teeth and tooth-like structures, together named odontodes, are repeated organs thought to share a common evolutionary origin. These structures can be found in gnathostomes at different locations along the body: oral teeth in the jaws, teeth and denticles in the oral-pharyngeal cavity, and dermal denticles on elasmobranch skin. We, and other colleagues, had previously shown that teeth in any location were serially homologous because: i) pharyngeal and oral teeth develop through a common developmental module; and ii) the expression patterns of the Dlx genes during odontogenesis were highly divergent between species but almost identical between oral and pharyngeal dentitions within the same species. Here we examine Dlx gene expression in oral teeth and dermal denticles in order to test the hypothesis of serial homology between these odontodes.


We present a detailed comparison of the first developing teeth and dermal denticles (caudal primary scales) of the dogfish (Scyliorhinus canicula) and show that both odontodes develop through identical stages that correspond to the common stages of oral and pharyngeal odontogenesis. We identified six Dlx paralogs in the dogfish and found that three showed strong transcription in teeth and dermal denticles (Dlx3, Dlx4 and Dlx5) whereas a weak expression was detected for Dlx1 in dermal denticles and teeth, and for Dlx2 in dermal denticles. Very few differences in Dlx expression patterns could be detected between tooth and dermal denticle development, except for the absence of Dlx2 expression in teeth.


Taken together, our histological and expression data strongly suggest that teeth and dermal denticles develop from the same developmental module and under the control of the same set of Dlx genes. Teeth and dermal denticles should therefore be considered as serial homologs developing through the initiation of a common gene regulatory network (GRN) at several body locations. This mechanism of heterotopy supports the 'inside and out' model that has been recently proposed for odontode evolution.


Teeth and tooth-like structures, together designated as odontodes, are thought to be serial homologs: they are repeated mineralized units composed of dentine and enamel, or enameloid, surrounding a pulp cavity [[1, 2], see for review: [3]]. Odontodes can be found in various locations on the body of extant gnathostomes, such as teeth in jaws and different bones in the oral and pharyngeal cavity, but also as dermal denticles (also called placoid scales) on the body surface in chondrichthyans [4]. Teeth (oral or pharyngeal) contrast with denticles (pharyngeal or dermal) in their ability to regenerate through a typical renewing process [5]. There has been a long and recently revitalized debate concerning the origin and evolution of odontodes. Due to their mineralized composition, they are well preserved in the fossil record and a diversity of odontodes has been described in fossil and extant taxa belonging to gnathostomes: dermal denticles in thelodonts or heterostracans, pharyngeal denticles/teeth in thelodonts, ornaments on dermal bones of placoderms or coelacanths, or the earliest oral teeth described in placoderms [6]. More controversial are the pharyngeal denticles/teeth found in conodont animals that are currently considered to have diverged early from other vertebrates [7, 8]. The long-held view [1] that oral teeth first evolved by the co-option of dermal denticles at the oral margin when jaws evolved (the outside-in hypothesis) has been challenged by reconsideration of these fossil data. Because pharyngeal denticles may have arisen before oral teeth and because both structures share a common organization, Smith and Coates [9, 10] favoured a recruitment of the gene regulatory network (GRN) responsible for pharyngeal teeth development from the pharynx to the jaw in early gnathostomes (the inside-out hypothesis). This model has been supported by morphological and molecular data gained in teleosts: pharyngeal tooth development has been compared to that of the mouse oral teeth, showing that similar signalling and transcription factors are expressed during oral and pharyngeal odontogenesis [1113]. However, detailed comparison of expression patterns between zebrafish pharyngeal and mouse oral tooth development showed differences and some molecular markers are specific for mouse oral (Pax9 [13, 14]) or zebrafish pharyngeal (eve1 [15]) odontogenesis. Additional studies have focused on comparative analysis between oral and pharyngeal dentitions within a given organism. They showed that, in extant teleost fish, teeth in the jaw or in the pharynx develop through similar gene expression patterns [11, 1618]. These results support the hypothesis that a single developmental GRN is initiated at different locations to make up oral and pharyngeal teeth through a simple mechanism of heterotopy [18]. These studies led to a more comprehensive scenario about odontode origin and evolution (named the "inside and out" model) that postulates serial homology between all gnathostome odontodes, as defined by the sharing of a common GRN for their development [19]. Oral teeth, pharyngeal teeth/denticles, and dermal denticles would then belong to this odontode group, developmentally characterized by: (i) the presence of a neural-crest derived mesenchyme; (ii) any epithelium able to respond to a mesenchyme signal [19].

In order to test the "inside and out" model, we searched for both developmental and genetic similarities between dermal denticles and oral teeth in the dogfish, Scyliorhinus canicula. In this species, histological observations support that tooth and dermal denticle development display similarities with that of osteichthyans [20, 21]. Among the different subsets of dermal denticle described during dogfish embryogenesis by Mellinger and Wrisez [22], we chose to work on the earliest developing ones, the caudal primary scales. They are located at the very tip of the tail, develop from caudal to rostral, are clearly observable in 28 mm long embryos, and are organised as four bilateral lines (two dorsal and two ventral lines with usually ten and eight scales respectively). Currently, only few expression data have been described for tooth and dermal denticles development in chondrichthyans: Shh [23], Epha4 [24], Runx1 and Runx3 [25], each gene showing a similar expression pattern in both structures. These expression patterns were not characterized on histological sections, therefore the tissue-specific transcriptional dynamics (epithelium vs mesenchyme) cannot be compared between structures or to other gnathostome species. To test the hypothesis of serial homology between tooth and dermal denticle development, we have characterised the expression of all Dlx gene family members identified in the dogfish, following a strategy that already allowed us to propose that a single GRN is involved in both oral and pharyngeal teeth in medaka [18]. This gene family represents a paradigmatic genetic marker to test if one or two independent GRN are involved in tooth-like structures because: (i) this gene family includes at least six members in gnathostomes, transcribed with different expression patterns during tooth development in the mouse [26, 27] and teleosts [12, 18, 28], and (ii) contrary to the variability of Dlx patterns between species, the regulation of Dlx expression patterns is not dissociated between the different dentitions within a given organism [18].

We show here that the first developing teeth and caudal primary scales form through four common typical stages that correspond to the common stages we previously identified for oral and pharyngeal odontogenesis in mouse and teleosts [12, 17]. We have identified six Dlx genes in the dogfish and analysed their expression patterns in teeth and caudal primary scales at the histological level. Three of them showed strong transcription in both structures (Dlx3, Dlx4 and Dlx5) whereas lower transcription levels could be detected for Dlx1 in dermal denticles and teeth, and for Dlx2 in dermal denticles. We observed very little difference in the transcription patterns of a given Dlx gene between teeth and caudal primary scales, except for the lack of Dlx2 transcription specifically in tooth buds. These results strongly suggest that a single set of Dlx genes is involved in oral tooth and dermal denticle development in the dogfish and therefore strongly support the hypothesis of serial homology between these odontodes. In this context, we propose that Dlx genes belong to a core set of developmental genes involved in all odontode development in gnathostomes. Our results imply that the GRN involved in odontode formation could have been initiated at several location (skin, mouth, oral cavity, pharynx) by simple heterotopy during the course of evolution and therefore represent the first detailed molecular support for the "inside and out" model.


Tooth and dermal denticle development

As mentioned by Mellinger and Wrisez [22] the first developing dermal denticles (caudal primary scales) and teeth could be observed at the very tip of the tail and the lower jaw, respectively (Figure 1A, insets). In order to follow the pattern of mineralization in the first developing teeth and dermal denticles, jaws and tails dissected from dogfish embryos measuring from 2 cm to 9 cm were stained with alizarin red (Figure 1B-F). At the tip of the tail, buds of caudal primary scales were first observable in 2.5 cm long embryos. As previously described [22], caudal scale development progressed in a posterior to anterior wave in the terminal part of the tail: 3 cm-long embryos showed alizarin red staining only in the very caudal scales while very young scale buds were still at early stage of development in the anterior part of the primary scale-forming area (Figure 1B-C). In 3.4 cm long embryo, four scales were mineralized in the dorsal and ventral parts of the tail (not shown). In 4 cm long (not shown) and older embryos, the full set of caudal primary scales was stained with alizarin red (Figure 1A, D,) although the most rostral scales were not erupted yet (not shown). We chose to name these scales Dorsal or Ventral Caudal Scales (dcs or vcs) respectively -1 to -10 and -1 to -8, from caudal to rostral: number refers to the rank of apparition during development, but few variations in scale number were observed between specimens.

Figure 1

Localization and embryonic development of caudal primary scales and oral teeth in the dogfish. A: ventro-lateral view (anterior is to the left) of a 6 cm long embryo stained with alcian blue (cartilage) and alizarin red (prismatic calcified cartilage and dentine); insets: left panel is a ventral view (anterior is to the top) of the lower jaw showing the first mineralized tooth (white arrowhead), right panel is a close-up lateral view (anterior is to the left) of the very tip of the tail showing the bilateral dorsal and ventral rows of caudal primary scales. B-D: caudal primary scale organisation, anterior is to the left and dorsal to the top in all pictures. B: left lateral view of the tail of a 3 cm long embryo after alizarin red staining showing seven buds in the dorsal row and six on the ventral one. Rostral buds are less developed than caudal ones; only the caudal-most buds show alizarin red staining (close up in C, arrowhead). D: left lateral view of the tail of a 7 cm long embryo showing alizarin red stained caudal primary scales. All primary scales are fully developed. Primary scales of the right side are out of focus. E-F: dorsal views of lower jaws after alizarin red staining, anterior is to the top; E: 7 cm long embryo, the four first teeth of the first row are stained (arrowheads) and the following are beginning to mineralize (arrow); F: 7.5 cm long, teeth of the second row (arrows) intercalate between teeth of the first row. Scale bars: A: 0.4 cm; insets 400 μm; B: 200 μm; C: 100 μm; D-F: 400 μm; dcs: dorsal caudal scale, vcs: ventral caudal scales, s: symphysis.

The detailed pattern of tooth development was difficult to identify by alizarin red staining: the first mineralized tooth could be detected in embryos reaching 6 cm long while there were at least five on each quadrant in 7 cm long embryos (Figure 1A, E). Despite some individual variations, the first tooth bud generally appeared lateral to the symphysis, and then two other tooth buds developed on both sides of the first tooth. Additional teeth subsequently developed on the jaw margin, from the symphyseal (distal) portion towards the articulation (proximal). New tooth buds also developed between teeth of the first row, in a more posterior second row of teeth, starting in 7.5 cm long embryo (Figure 1F). A third row of tooth buds was observable in 8.5 cm long embryos (not shown).

Histological characterization of tooth and dermal denticle developmental stages

In order to depict the modifications of the mesenchymal and epithelial compartments through tooth and dermal denticle development, we performed histological sections and Nissl stain (cresyl violet - thionin) coloration on dissected tails of 3 cm long embryos and on lower jaws for embryos ranging from 4 cm to 5.5 cm long (Figure 2).

Figure 2

Histological definition of four developmental stages for caudal primary scale and tooth development. Transversal sections of a tail from a 3 cm long embryo (A-E) and of lower jaws from 4 cm (F-H) and 5.5 cm (I-J) long embryos coloured with Nissl stain (cresyl violet - thionin), scale bar: 25 μm. K: diagram summarising the four common stages for odontode development in the dogfish. EM, early morphogenesis- thickening of the dental epithelium (d.e.), condensation of the mesenchyme (m, coloured grey); LM, late morphogenesis- bell shape bud formation; ED, early differentiation- a constriction appears at the bottom of the bud (arrowheads); LD, late differentiation- matrix secretion (arrow).

The first developing oral tooth and caudal primary scale buds showed the same successive cellular and histological stages that those previously shown to be common between oral and pharyngeal tooth development in osteichthyans [12, 17]. In addition, cellular and histological aspects of scale bud development were similar to tooth bud stages. In both structures, the early morphogenesis stage (EM) started with the placode formation: the shape of odontogenic epithelial cells changed from cubic to prismatic and the underlying mesenchyme began to condensate (Figure 2A, F). At late morphogenesis stage (LM), the epithelium progressively folded and enclosed the mesenchymal compartment resulting in a bell-shaped bud. At that stage, we could distinguish between early LM stage when the epithelium started to fold (Figure 2B, G) and late LM stage when the epithelium fold was more pronounced and the bud exhibited a typical bell shape (Figure 2C, H). The third stage, ED (early differentiation), was characterized by a constriction that could be observed at the basis of the bell on both scale and tooth buds (Figure 2D, I). During the last stage, LD (late differentiation), epithelial cells had their nucleus shifted towards the apical pole and showed secreting vesicles in their basal cytoplasm. The first signs of matrix deposition confirmed that ameloblasts were fully differentiated (Figure 2E, J). Differentiation of the odontoblasts was not included in this analysis as no histological sign could be identified showing their synthesis activity. These observations are summarised in the diagram in Figure 2K.

Overall expression of Dlxgenes in first forming teeth and dermal denticles

Two segments for each Dlx coding sequences were amplified from the dogfish genome by degenerate PCR based on the six Dlx sequences identified in Triakis semifasciata [29]. These segments were concatenated and phylogenetic analyses were performed to check their orthology to gnathostome Dlx1 to Dlx6 (see Additional file 1). The amplified sequences were used to synthesize anti-sense RNA probes against the Dlx mRNAs in the dogfish (Additional file 2).

We performed in situ hybridizations on dissected tails of 2.5 cm to 3 cm long embryos (stage 29-31) and on lower jaws for embryos ranging from 4 cm to 5.5 cm long, in order to describe gene expression in the first developing teeth and caudal primary scales through their whole developing process. Transcripts were detected at high levels in both structures for three out of six Dlx genes: Dlx3, Dlx4 and Dlx5 (Figure 3E-J). Lower levels of expression could be detected with the Dlx1 and Dlx2 probes in developing caudal primary scales, and with Dlx1 probes in developing teeth (Figure 3A-D). Dlx6 transcripts were never detected, either in tooth or scale buds (not shown).

Figure 3

Whole mount views of Dlx gene expression during caudal primary scale and tooth development. In situ hybridizations against Dlx1 (A-B), Dlx2 (C-D), Dlx3 (E-F), Dlx4 (G-H) and Dlx5 (I-J) mRNAs. Left lateral view of tails from embryos at stage 30 (A, C, E, G and I), anterior to the left, dorsal to the top. Four dorsal scale buds and three ventral buds are stained in A, five dorsal and four ventral scale buds in C, E, G and I. Dorsal views of lower jaws (B, D, F, H and J), anterior to the top, from 4.9 cm (B and D), 4.5 cm (F), 4.6 cm (H) and 4.7 cm (J) long embryos. Scale bars: 200 μm, s: symphysis.

Tissue specific expression of Dlxgenes during dermal denticle development

In order to obtain a detailed description of the Dlx gene expression patterns during the development of dermal denticles in the dogfish, we prepared histological sections of the whole-mount hybridized tails, and assigned each caudal primary scale bud to one of the four stages of development described in Figure 2. Caudal primary scale development lasts over weeks and expression patterns may change along a given developmental stage. We observed as many buds as possible for each stage, and separated the LM into an early and a late LM, in order to obtain a more detailed view of the dynamic changes of expression patterns. For a given probe, the expression dynamic is identical in at least the sixteen first caudal primary scales (four first dorsal and ventral caudal scales on each lateral side), so the values given for each stage in the following paragraph result from pooling together our observations as summarized in Table 1.

Table 1 Occurrence of positive staining in the epithelial and mesenchymal compartments of developing caudal primary scale buds after in situ hybridization

During caudal primary scale development, we detected low levels of Dlx3 transcripts in the thickened epithelium of all bud placodes (EM, n = 25/25, Figure 4A). Dlx3 transcripts were then detected at higher levels in the epithelial compartment of both the LM stage and ED stage (n = 28/28 and n = 26/26, respectively, Figure 4B, C). Finally, transcription of Dlx3 was detected only in the epithelial layer in the beginning of the LD stage (n = 28/28, Figure 4D). Dlx3 transcripts were detected in the mesenchyme in only some of the scale buds during the LM stage (n = 16/28): early LM group showed both negative and positive buds (n = 7 positive out of 19 early LM) while positive staining was always observed in late LM buds (n = 9/9, Figure 4B). Transcription of Dlx3 was therefore progressively initiated during the beginning of the LM stage. Dlx3 transcripts were apparent in the mesenchyme of ED scale buds (n = 20/26) but undetectable in LD stages, showing a negative regulation of Dlx3 during the LD stage (Figure 4C-D).

Figure 4

Histological analysis of Dlx gene expression patterns during caudal primary scale bud development. Transversal sections of whole mount hybridized tails from embryos at stages 29 to 31 illustrating the expression patterns of Dlx3 (A-D), Dlx4 (E-H), Dlx5 (I-L), Dlx1 (M-P) and Dlx2 (Q-T) during the development of the scales. Embryos are at stages 29 (B, E, F, I, M, N and Q), 30 (A, C, G, H, L, O, R-T) and 31 (D, K and P). EM, LM, ED and LD legends are as in Figure 2. For the LM stage, we illustrate the late LM stage when Dlx genes are predominantly expressed in the mesenchyme of scale buds. Dotted lines indicate the basal lamina separating the dental epithelium and the underneath mesenchyme. Scale bars: 25 μm.

Transcripts of Dlx4 were detected with the same expression pattern: in the epithelium of caudal primary scale buds during the EM (n = 20/20, Figure 4E), LM (n = 65/65, Figure 4F), ED (n = 36/36, Figure 4G) and LD (n = 22/22, Figure 4H) stages and in the mesenchyme of scale buds at the LM (34/65), ED (31/36) and LD (n = 6/22). Again, during the LM stage, positive staining was mainly observed in more late LM buds (n = 29 positive out of 30 late LM stage, Figure 4F) while positive buds were rarely observed in the early LM group (n = 5 positive out of 35 early LM). Positive staining in the mesenchyme of LD stages was more frequently observed in less developed buds, suggesting that Dlx4 expression is turned off early during LD.

We also detected Dlx5 transcripts in the epithelium of caudal primary scale bud during the four stages of development (EM, n = 18/18; LM, n = 53/53; ED n = 35/35; LD, n = 26/26), and in the mesenchymal compartment of the LM (n = 20/53 positive, of which n = 20 in the late LM stages out of 26 late LM buds), ED (n = 35/35) and LD (n = 13/26) stages (Figure 4I-L).

Histological sections on hybridized tails revealed no detectable expression of Dlx6 at any stage (not shown), while Dlx1 and Dlx2 transcripts were detected at low intensity (Figure 4M-T). We used these same Dlx probes on whole-mount embryos at early stage of organogenesis (see Additional file 3), and could get strong signals for every Dlx genes here identified (note that probe lengths are equivalent for all six genes). We concluded that our results with Dlx1 and Dlx2 probes were not due to experiment or probe artefact, but most likely were a consequence of low expression levels for these two genes during caudal primary scale development. Dlx1 transcripts were detected in the epithelium of scale buds at the LM (n = 5/16, all of them from the late LM group, 10 late LM buds), ED (n = 19/34) and LD (n = 29/34) stages as well as in the mesenchyme of ED (n = 11/34) scale buds (Figure 4M-P), showing a progressive up-regulation of Dlx1 in the late LM/early ED stage. Dlx2 transcripts were detected in the epithelium of caudal primary scale buds at the EM (n = 11/13), LM (n = 31/36), ED (n = 16/28) and LD (n = 9/11) stages. Dlx2 expression was also detected in the mesenchymal compartment of scale buds during LM (n = 23/36) stage, most of the positive buds being in the late LM group (n = 17 out of 19 late LM buds). Transcripts were then detected during ED (n = 27/28) stage but undetectable in LD (Figure 4Q-T), showing activation of Dlx2 transcription during the LM stage and down-regulation early in the LD stage. The dynamics of each Dlx gene expression in the epithelial and mesenchymal compartments of primary scale buds are graphically illustrated in Figure 5A and 5C.

Figure 5

Dynamics of Dlx gene expression in the epithelial and mesenchymal compartments of developing caudal primary scales and teeth. Graphical representation of data presented in Tables 1 and 2. For each gene and each developmental stage, the percentage of positive buds over the total number of observed buds is plotted and a curve connecting these plots is drawn. Epithelial (A, B) and mesenchymal (C, D) compartments in caudal primary scales (A, C) or teeth (B, D). See legend of Figure 2 for abbreviations of the developmental stages.

Tissue specific expression of Dlxgenes during tooth development

As described for dermal denticles, we pooled our observations for the eight first teeth (four first teeth on each hemi-jaw) and summarized our results in Table 2. During tooth development, Dlx3, Dlx4 and Dlx5 transcription started in the epithelial compartment of EM buds and was maintained in LM, ED and LD (Figure 6A-L and Figure 5B). Expression in the mesenchymal compartment started during the LM stage (Dlx3, n = 17/31; Dlx4, n = 14/22; Dlx5, n = 10/37) and most of the positive buds were found in the late LM group (Dlx3, n = 13/17, Figure 6B; Dlx4, n = 10/10, Figure 6F; Dlx5, n = 10/17, Figure 6J and 5D). Expression in the mesenchymal compartment was maximum for Dlx3 and Dlx4 during the ED stage (n = 12/18 and n = 13/13, respectively, Figure 6C, G and 5D) but down regulation of their expression was apparent during the LD stage (positive tooth buds: n = 7/13 for Dlx3, Figure 6D, and n = 7/21 for Dlx4, Figure 6H and 5D). Dlx5 transcripts were detected in the mesenchyme of some of the tooth buds at the ED stage (n = 14/20, Figure 6K) and was maximal in LD stage buds (n = 11/14, Figure 6L and 5D). Expression of Dlx2 and Dlx6 could not be significantly detected by in situ hybridization followed by histological sections. Among 42 Dlx2 hybridized teeth, we were able to detect a very weak signal in the epithelium during the LM stage of six developing tooth buds. We decided to consider that these data were not significant, but we cannot exclude that the level of Dlx2 transcription was too low to be detected by the in situ hybridization technique. On the contrary, Dlx1consistently showed low levels of expression in developing tooth buds: transcripts were detected in the epithelium of all four stages of development (EM, n = 5/5, Figure 6M; LM, n = 25/26, Figure 6N; ED, n = 12/12, Figure 6O; LD, n = 6/6, Figure 6P; see also Figure 5B), and transient expression in the mesenchyme of ED buds could be observed (n = 7/12, Figure 6O and 5D).

Table 2 Occurrence of positive staining in the epithelial and mesenchymal compartments of developing tooth buds after in situ hybridization
Figure 6

Histological analysis of Dlx gene expression patterns during tooth development. Transversal sections of hybridized lower jaws of 4 cm to 5.5 cm long embryos illustrating the expression patterns of Dlx3 (A-D), Dlx4 (E-H), Dlx5 (I-L) and Dlx1 (M-P) during the development of oral teeth. Lower jaws of 4 cm long embryos (A, I and M), 4.3 cm (E), 4.5 cm (B, F, and J), 4.6 cm (G, N and O), 5 cm (C, D and K), 5.1 cm (P), 5.4 cm (H) and 5.5 cm (L). We illustrate only the late LM stage when Dlx genes are predominantly expressed in the mesenchyme of tooth buds. Legends are as in Figure 2 and 4. Scale bar: 25 μm.


Dermal denticle and tooth development progress through common developmental stages

It has long been considered that oral teeth and dermal denticles display developmental similarities [13]. The histological data we obtained for embryonic oral tooth and caudal primary scale development in the dogfish are in accordance with those obtained previously in other elasmobranch species or at later stages of dogfish development [20, 21]. In addition, our morphological and histological observations show that teeth and scales share morphogenetic similarities through four developmental stages, which are also shared with mammalian oral teeth and teleost pharyngeal or oral teeth [12, 17, 30]. As a consequence, we postulate here that all odontodes, whatever their location, develop through four common successive stages including the formation of a placode (EM), shaping of the bud (LM), differentiation of ameloblasts and odontoblasts (ED) (as defined in osteichthyans and as deduced from the observation of fully functional ameloblasts in LD buds in the dogfish), and matrix deposition (LD). Similar organization and composition of the fully developed teeth and dermal denticles, as well as similar stages of development, support the hypothesis of serial homology between epithelial mineralized structures.

Serial homology of odontodes is molecularly supported by non-dissociation of Dlxexpression patterns in caudal primary scales and teeth

To further explore the potential link of serial homology between odontodes we analysed the expression pattern of six Dlx genes in Scyliorhinus canicula. We assumed that these six genes constitute the whole set of Dlx genes in the dogfish, which also was the probable ancestral state in gnathostomes [29], even if we cannot exclude that individual duplications occurred in the dogfish lineage. No expression of Dlx6 could be detected in either tooth or scale buds. Dlx3, Dlx4 and Dlx5 were expressed in the epithelial compartment during all four common stages of caudal primary scale and tooth development (Figure 5). In the mesenchymal compartment, transcription of Dlx3, Dlx4 and Dlx5 was initiated during the LM stage and then down-regulated shortly after the ED stage for Dlx3 and Dlx4 while expression of Dlx5 was still on during the LD stage. The expression dynamics of each of these three genes show only subtle differences between caudal primary scale and tooth. First, more than 40% of the tooth buds we examined show no transcription of Dlx5 in the epithelium during the initiation of expression at EM stage whereas it is expressed in the epithelium of all caudal primary scale buds (Figure 5A and 5B). Similarly, Dlx5 transcription in the mesenchyme is never detected in 100% of tooth buds as it is in scale buds (Figure 5C and 5D). Second, the dynamic of transcription of Dlx3 is identical in the epithelium of both tooth and caudal primary scale (Figure 5A and 5B) but the expression in the mesenchyme at ED is detectable in all scale buds stage but only in about 80% of the tooth buds (Figure 5C and 5D). Expression of Dlx1 was also similar in the mesenchymal compartment of both caudal primary scale and tooth buds, with transient expression during the ED stage. However, the Dlx1 expression pattern showed heterochrony in the epithelial compartment as transcription started during the LM stage in scale buds, while it was already active in the EM stage in tooth buds. Note that Dlx1 signal was very weak compared to Dlx3, Dlx4 and Dlx5 and we cannot exclude that Dlx1 expression was too weak to be detected during the early morphogenesis stages. The main difference observed in this study is the complete lack of expression of Dlx2 during tooth development, while the expression dynamic of this gene was similar (although weaker) to those of Dlx3 and Dlx4 during scale bud development. In conclusion, we show that the tissue specific expression patterns of each Dlx genes are nearly identical (except for Dlx2) during oral tooth and dermal denticle development in the dogfish. Taken together with our previous data on oral and pharyngeal dentitions, the results presented here show that the Dlx genes expression patterns did not undergo dissociation between odontodes (teeth, denticles, scales) that form at different locations (mouth, pharynx, skin) in a given species. Therefore, all odontodes of a given organism appear to be serially homologous because they bud through the redeployment of common developmental stages associated with a similarly regulated set of Dlx genes.

Different Dlxexpression patterns during odontode development among jawed vertebrates

In order to gain insights into the evolution of Dlx expression patterns during gnathostome odontode development we compared our results with those published in mouse [26], zebrafish [12] and medaka [18]. A summary of expression patterns published for the mouse Mus musculus (m), zebrafish Danio rerio (d), medaka Oryzias latipes (o) and dogfish Scyliorhinus canicula (s) are presented in Table 3. Note that the results recently obtained for Dlx expression during pharyngeal tooth development in an African cichlid (Astatotilapia burtoni) [28] were consistent with what had previously described for Dlx expression in the medaka and were therefore omitted in the Table 3 in order to clarify the analysis. Contrasting with the non dissociation of Dlx expression patterns in odontodes within a given species (this work and [18]), the set of genes expressed varies highly between species: Dlx1 is never expressed during zebrafish or medaka odontogenesis while Dlx6 is not expressed during dogfish or zebrafish odontogenesis. This observation suggests that a specific combination of Dlx genes was not strictly constrained during gnathostome evolution, probably because the redundancy between the different paralogs favoured function shuffling. On the other hand, Dlx2, 3, 4, and 5 are transcribed in all three species even though their expression patterns vary, especially in the epithelial compartment. Common points are restricted to: (i) early epithelial transcription for Dlx3 (EM); (ii) mesenchymal transcription for all four genes during LM and ED (note two exceptions, no expression of dlx4b in the mesenchyme of LM buds in medaka, no expression of dlx3b in the mesenchyme of ED buds in zebrafish); (iii) late (LD) mesenchymal transcription for Dlx5. Given the homology relationship proposed between gnathostome odontodes, these similar features might be inherited from the gnathostome last common ancestor (see the ancestral expression pattern, hypothesis a, in Figure 7). Another trend is highlighted with this comparison: expression of Dlx3 and Dlx5 in the epithelium at all stages is observed in all species but the mouse (except for Dlx3 expression in EM stage).

Table 3 Dlx gene expression patterns during odontode development in gnathostomes
Figure 7

Theoretical tree of the relationships between extant gnathostomes and fossil species. The evolutionary scenario described in the discussion is illustrated, including the diversification of vertebrate odontodes and hypotheses for the modification of Dlx gene expression patterns over time: a- proposed ancestral situation (note that the early Dlx2 mesenchymal expression depends on the alternative hypotheses in c); b- loss of epithelial Dlx3 and Dlx5 expression; c- two equi-parsimonious hypotheses: c1 = gain in bony fish/c2 = loss in chondrichthyans of the Dlx2 early mesenchymal expression; d- loss of Dlx2 expression in teeth but not in dermal denticles.

This may be interpreted as a specific loss in the lineage leading to the mouse (sarcopterygian), and could even be viewed as a single evolutionary event if one considers that the epithelial expression of Dlx3 and Dlx5 is up-regulated by one single activator. If considered as a single evolutionary event, the most parsimonious scenario to explain these data would be that Dlx3 and Dlx5 were transcribed in the epithelium of odontode buds at all stages in the gnathostome last common ancestor, and that this trait was lost secondarily in the sarcopterygian lineage leading to the mouse (see hypothesis b, in Figure 7). This result could be correlated to the different roles taken by the epithelial compartment in sarcopterygians as opposed to non-sarcopterygians [31]. In non-sarcopterygians (chondrichthyans and actinopterygians), the outer-most layer of mineralized tissue (enameloid) is synthesized by a cooperation between the mesenchymal (neural-crest derived odontoblasts) and the epithelial (ameloblasts) compartments [32]. On the contrary, the sarcopterygian outer mineralized layer (best described in amniotes) is composed of enamel, which has been showed to be synthesized exclusively from the epithelial layer of ameloblasts with specific secretory characteristics [33].

Another prominent result is the lack of early Dlx2 expression (during EM stage) in the mesenchyme of tooth and dermal denticle buds in the dogfish. This early expression in the mesenchymal compartment was observed in the mouse, zebrafish and medaka, and is a specificity of the Dlx2 orthologs. Two equi-parsimonious scenarios can be proposed: (i) the early mesenchymal expression of Dlx2 is a novelty acquired in the last common ancestor of bony fish only; (ii) the early mesenchymal expression of Dlx2 is an ancestral gnathostome characteristic, and it has been lost in the chondrichthyan lineage (hypotheses c1 and c2 in Figure 7). Note that the lack of Dlx2 expression in dogfish teeth is most probably a derived state (hypothesis d in Figure 7) as Dlx2 expression is detected in dogfish scales. Other data presented in Table 3 do not allow to propose one most parsimonious scenario for expression pattern evolution: one-species characteristic i.e. apomorphy, or two-species characteristic leading to convergence (mouse (m) + medaka (o), or zebrafish (d) + dogfish (s)).


Here, we present a detailed comparison of tooth and dermal denticle development in the dogfish that show that odontodes should accurately be considered as serial homologous structures. Indeed in a given species, the development of the different odontodes involves (i) the localised redeployment of the same developmental stages, and (ii) the localised redeployment of the same Dlx expression patterns (but Dlx2). We reasoned that, if an ancestral regulation of Dlx expression had dissociated and evolved independently in the different odontodes after its cooption at different locations, there should be more differences between expression patterns when comparing structures in one organism (serial homology) than when comparing homologous structures between extant gnathostomes (specific homology). However, we observe more divergence between the Dlx expression patterns of different species than between serial homologous structures within a species such as dogfish teeth and dermal denticles (see Table 3) or teleost oral and pharyngeal teeth, as shown in the Additional file 4. We conclude that the reiterated expression of the dynamic Dlx patterns in teeth and dermal denticles within a given organism reveals that the different odontodes form from the reiterated expression of a single GRN. Our results therefore provide the first detailed molecular supports for the "inside and out" model [19] that propose that all odontodes are serially homologous structures that form through the control of a common odontode gene regulatory network (oGRN). This ancestral oGRN may include, in addition to the Dlx family, several other genes such as Shh, Epha4, and Runx, that were shown to be expressed during both scale and tooth development in chondrichthyans [2325], and during oral and/or pharyngeal tooth development in mouse and/or teleost fish [34, 35]. The evolutionary scenario for the relative time of appearance of the different odontodes depends on the phyletic relationships between fossil agnathans such as conodonts and thelodonts, extant agnathans, and extant gnathostomes, which remain controversial [7, 8, 36]. Our results support the hypothesis that the GRN involved in the development of serially homologous odontodes has not dissociated over gnathostome evolution. This implies that, during the course of evolution, the diversity of gnathostome odontodes arose from independent gains of expression territories of a single ancestral GRN by simple heterotopy. Therefore, molecular developmental data would not allow comparing odontodes in order to evaluate their divergence over evolutionary time. As a consequence, only additional paleontological data can shed a new light on the evolutionary events leading to the diversity of odontodes.



Dogfish (Scyliorhinus canicula) embryos were obtained from the Station de biologie marine (Roscoff, France, CNRS and MNHN). All embryos were maintained at 17°C in sea water until they reach the correct developmental stage, defined by their total length. They were dissected and then fixed 48 hours at 4°C in a phosphate buffered saline solution containing 4% paraformaldehyde (PFA). Embryos were then dehydrated in methanol and stored at -20°C.

Alizarin red staining

Whole-mount embryos stored in methanol were progressively transferred in 0.5% KOH solution. They were then coloured over-night in an alizarin red solution (0.001%) in 0.5% KOH at room temperature. After their progressive transfer to glycerol, they were photographed under bright field.

Double alcian blue and alizarin red staining

Whole-mount embryos (more than 5 cm long) were fixed in buffered 10% formaldehyde for a day, then rinsed in distilled water and transferred to 70% ethanol for storage. They were stained in filtered alcian blue solution (alcian blue 200 mg/L in 70% ethanol-30% glacial acetic acid) for 48 hours, rinsed in decreasing ethanol bathes, to distilled water, and then in a 30% saturated sodium borate solution. They were then digested in a trypsin solution (1% in 30% saturated sodium borate solution, renewed when blue) until the specimen is cleared. Specimens were then transferred in a 0.5% KOH solution to be stained with alizarin red.

Cloning Dlxcoding sequences

Amplification of the first and third exon of each Dlx coding sequence was made with degenerated primers designed on the published sequences of Triakis semifasciata Dlx genes [29]. Dlx probe positions are shown on Additional file 2, and primer sequences are given in Additional file 5. PCR products were cloned in pGEM-T Easy vector (Promega). Antisense RNA digoxigenin-UTP probes were prepared using SP6 or T7 polymerase, according to the orientation of the insert in the plasmid.

Histological sectioning

Dissected jaws and tails from embryos stored in methanol at -20°C were put through several baths of absolute ethanol, then in butanol and finally embedded in paraplast for 10 μm cross-sections. Sections were then coloured with Nissl stain (cresyl violet - thionin).

Whole mount in situhybridizations

Whole mount in situ hybridizations were performed according to standard protocol [24] using two antisens RNA probes for each assay on dissected jaws (from 4 to 5.5 cm embryos long) or tails (from 2.5 to 3 cm embryos long). Proteinase K treatments (10 μg/mL) were adapted for dissected jaws and tails: 30 min at room temperature for tails, twice 30 min for jaws. The colour detection step was performed using the NBT-BCIP reaction. We assayed this protocol on at least four embryos for each developmental stage and with positive control (earlier embryo with known restricted expression pattern with the same probe, see Additional file 3).

Expression pattern analysis

The dissected jaws and tails were post-fixed in 4% PFA after whole mount in situ hybridization, then cleared and stored in glycerol at 4°C to be photographed under bright field. Whole-mount hybridized dissections were put through several baths of absolute ethanol, then in butanol and finally embedded in paraplast for 10 μm cross-sections. Negative whole-mount detections were also verified after histological sections.


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We would like to thank Elena Luchetti for help with adult dogfish keeping and embryo collection, Sylvie Mazan and Marion Coolen for help with in situ hybridization, Isabelle Germon for in situ hybridizations, and Jacob Pollack for improvement of the manuscript. This work was supported by grants from the Centre National de la Recherche Scientifique (CNRS, ATIP), from the GIS (Génomique marine) and from the Université Paris-Sud 11 (BQR). SO and MDT were supported by a doctoral fellowship from the French Ministère de l'Education Nationale et de la Recherche

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Correspondence to Véronique Borday-Birraux.

Additional information

Authors' contributions

MDT, VBB, SO and FB produced and analysed the morphological and in situ hybridization data. DC carried out the gene characterization and phylogenetic analyses. MDT, VBB and PL drafted the manuscript. VBB and DC designed the study. All authors have read and approved the manuscript.

Electronic supplementary material

Assignment of the

Additional file 1: Dlx probes to their group of orthologues. In order to assign each Dlx probe to an orthologous group, phylogenetic analyses were performed. The Dlx gene sequences of Triakis semifiasciata (leopard shark), Homo sapiens (human), Gallus gallus (chicken), Xenopus tropicalis (xenopus), Danio rerio (zebrafish) were retrieved from Ensembl release 49 and GenBank release 164. Multiple Dlx amino acid sequences were aligned using ClustalX (Thompson et al. 1997) with manual optimization using MUST software (Philippe 1993). Regions of ambiguous homology were removed. Evolutionary distances were computed using the Poisson correction and a neighbor-joining tree was obtained using MEGA4 (Tamura et al. 2007). The robustness of the tree nodes was estimated by a bootstrap test (1000 replicates). Philippe H. 1993. MUST, a computer package of Management Utilities for Sequences and Trees. Nucleic Acids Res 21:5264-5272. Tamura K, Dudley J, Nei M, and Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, and Higgins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882. (PDF 98 KB)


Additional file 2: Position of Dlxprobes on the coding sequences of T. semifasciata. The position of a probe is indicated by a grey line. The position of introns (I), the position of homeobox sequences (black box) and the length of each coding sequence (cds) and of each probe are indicated. (PDF 178 KB)

Test of

Additional file 3: Dlx probes activity during early stages of embryogenesis in the dogfish. Lateral view of whole mount in situ hybridized embryos with the Dlx probes designed for the 6 dogfish Dlx genes. For each panel, the name of the probe is indicated up right and the stage of the hybridized embryo down right. (PDF 503 KB)

Statistical evaluation of differences between

Additional file 4: Dlx expression patterns in gnathostome odontode development. A. Matrix describing the expression pattern of each Dlx gene from the mouse, zebrafish, medaka and dogfish, during all four odontode developmental stages (see text for a description), either in the epithelial (e) or mesenchymal (m) compartment. Data are as of Table 3, but different columns were made for oral (o) versus pharyngeal (p) teeth in medaka, or scales (s) versus oral teeth (o) in dogfish. B. Neighbor Joining tree inferred with a pairwise distance (number of differences) matrix estimated with the matrix of characters shown in A. (PDF 74 KB)

Primer sequences

Additional file 5: . Primer names follow the description in the Additional file 2. (PDF 48 KB)

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Debiais-Thibaud, M., Oulion, S., Bourrat, F. et al. The homology of odontodes in gnathostomes: insights from Dlx gene expression in the dogfish, Scyliorhinus canicula. BMC Evol Biol 11, 307 (2011).

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  • Gene Regulatory Network
  • Tooth Development
  • Epithelial Compartment
  • Early Morphogenesis
  • Dermal Denticle