Skip to main content

Reply to: “Research on agroforestry systems and biodiversity conservation: what can we conclude so far and what should we improve?” by Boinot et al. 2022

The Original Article was published on 03 March 2022

Abstract

In our article ‘European agroforestry has no unequivocal effect on biodiversity: a time-cumulative meta-analysis’ (BMC Ecology and Evolution, 2021) we synthesize the effect of agroforestry on biodiversity. Boinot et al. (BMC Ecology and Evolution, 2022) criticise our approach arguing that our definitions of agroforestry and biodiversity are too narrow; that we use inappropriate control sites for primary studies lacking distance to the treatment sites; that there are too few studies for a meta-analysis in silvoarable systems; and that local practice should be emphasized. We agree on the importance of local practices and that more studies would improve the robustness of our meta-analytical results. However, our conclusions are robust to removing studies criticised for inappropriate controls. We also recognize the problem of different definitions of agroforestry and using species richness as sole proxy for biodiversity. We appreciate being given the opportunity to clarify our results and to trigger future discussions about definitions and the interpretation of results from meta-analysis.

Peer Review reports

In our recent meta-analysis [1], we have shown that agroforestry has no overall positive effect on biodiversity, but we found an effect of small magnitude comparing agroforestry with cropland. In a comment, Boinot et al. [2] raised concerns about our definitions of agroforestry and biodiversity, the selection of the control sites used in our meta-analysis and the applicability of our results for conservation. We are grateful for stimulating a discussion about research quality in agroforestry and respond in the following to each of the points raised.

Hedges and agroforestry

Agroforestry is a land-use system combining agriculture or pasture with woody elements. Some definitions emphasize a mixture of trees with crops and/or animals on the same land and a combined production system [3, 4], while others also talk about agroforestry if trees and hedges are grown only at the border of crop fields and pastures [5]. Boinot et al. [2] especially argue for including hedges grown next to fields in the definition of agroforestry, which we did not cover with our meta-analysis. Today, hedges are generally not cultivated for wood production. Even in the example cited by Boinot et al., the majority of farmers stated to plant hedges for conservation and cultural purposes [2, 6]. Hedges offer habitat to different taxonomic groups and we would welcome a formal meta-analysis on the effect of hedges in different landscapes, in addition to existing reviews [7,8,9]. For our meta-analysis, we have chosen the narrower definition of agroforestry, only including systems with alternating trees and crops/pastures under the same management, to disentangle landscape structural elements and actual production systems.

Species richness as proxy for biodiversity

We agree with Boinot et al. that biodiversity has more dimensions than species richness alone. However, species richness is one very important aspect of biodiversity and showed to be related to abundance and biomass [10, 11]. It is further the measure for biodiversity most commonly used in the literature, thus best suited for including many studies in a meta-analysis. We want to emphasize that our main objective was not to provide a comprehensive conservation recommendation covering all potentially relevant aspects of biodiversity. We explicitly state in our article that we show the effects on biodiversity proxied predominantly by species richness.

Boinot et al. state that 22 of 28 studies found some effects of agroforestry on abundance, species composition or functional groups and hence our conclusion about biodiversity are not valid. Although we assume that their point was to emphasize the multidimensionality of biodiversity, we want to draw attention to the danger of such an argument. Vote counting is a statistically flawed and unreliable research synthesis method [12]. We have selected species richness as the most commonly reported measure of biodiversity, and hence most suitable for meta-analysis. Not having found an effect on one measure of biodiversity, i.e. species richness, and searching for another until one may be “significantly” positively affected, would constitute a case of p-hacking and thus an unacceptable scientific practice [13]. Others are welcome to extend our analyses to other measures, although we do not expect different results for abundance, functional or phylogenetic diversity, as they are typically highly correlated with species richness.

Control site selection

Boinot et al. criticise that some of our case-control comparisons on which the calculation of effect sizes is based, underestimate the effect of agroforestry on biodiversity. Indeed, we have included studies for which control locations were close to the treatment sites and this may potentially underestimate the effect of agroforestry on biodiversity. We have tested whether this was the case by excluding the sites that Boinot et al. regard as inappropriate [for code and data see Additional file 1 and 2]. The conclusion from the meta-analysis did not change after discarding the case-control comparisons criticized (Fig. 1).

Fig. 1
figure 1

Meta-analysis of a subset of studies from [1], excluding studies argued by [2] to have control locations too close to the treatment sites. Silvopasture was reduced by three effects sizes and silvoarable reduced from 17 to 11 effect sizes

Meta-analysis and local practices

For meta-analyses, a minimum of primary studies is required to compute a meaningful summary effect [14]. Boinot et al. point out that it is too early for a meta-analysis on alley cropping agroforestry systems given that there are few studies with an unbalanced sampling of age and taxonomic groups. As we have not computed a summary effect size for alley cropping and it is not even an existing category in our meta-analysis, we assume that silvoarable systems are meant here. We agree that too few studies in silvoarable systems exist for each age and taxonomic group to derive very robust conclusions.

However, in the same breath, Boinot et al. conclude that our meta-analysis ‘most likely underestimates the positive effects’. We wonder about such a conclusion on ‘likely positive effects’ that are necessarily based on the same few primary studies that are said to be too few for meta-analysis. In the absence of good primary studies, no general conclusions can be drawn in either direction and it is unjustified to assume that there must be a strong positive effect of agroforestry on biodiversity. We however think that even with a low number of primary studies, a quantitative synthesis in form of a meta-analysis will outcompete the statement of each individual study and allow more general and evidence-based conclusions [15]. Thus a systematic research synthesis increases the evidence base, even by including only few effect sizes [15]. We also agree that there is room for improvement and welcome future and complementary efforts on different aspects of land use, biodiversity and by increasing the number of primary studies.

The effect of agroforestry on biodiversity may be influenced by local differences as Boinot et al. point out. We agree that local differences should be considered, if they have an influence on the impact of agroforestry on biodiversity. We want to remind that a fundamental idea of evidence-based practice is the identification of a causal link between an impact, e.g. a conservation measure, and an outcome of interest [16]. These direct links are difficult to determine if unmeasured and locally differing variables additionally influence the outcome of interest, which is often the case in ecology. Consequently, the transferability of study results from one local context to another is not straightforward. Nevertheless, in the absence of knowledge about the local conditions under which agroforestry may be or not be beneficial for biodiversity, a meta-analysis across many local contexts synthesising systematically searched literature, is the best and most generalisable evidence we can have.

Availability of data and materials

All data required for the replication of the analysis are available in the supplementary material.

References

  1. Mupepele A-C, Keller M, Dormann CF. European agroforestry has no unequivocal effect on biodiversity: a time-cumulative meta-analysis. BMC Ecol Evol. 2021;21(193):1–12. https://doi.org/10.1186/s12862-021-01911-9.

    Article  Google Scholar 

  2. Boinot S, Barkaoui K, Mézière D, Lauri P-E, Sarthou J-P, Alignier A. Research on agroforestry systems and biodiversity conservation: what can we conclude so far and what should we improve? BMC Ecol Evol. 2022. https://doi.org/10.1186/s12862-022-01977-z.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Cardinael R, Chevallier T, Cambou A, Béral C, Barthès BG, Dupraz C, Durand C, Kouakoua E, Chenu C. Increased soil organic carbon stocks under agroforestry: a survey of six different sites in France. Agric Ecosyst Environ. 2017;236:243–55. https://doi.org/10.1016/j.agee.2016.12.011.

    Article  Google Scholar 

  4. Nair PKR, Kumar BM, Nair VD. An introduction to agroforestry, vol. 73. Cham: Springer; 2021. p. 279–81 (10.1007/978-3-030-75358-0).

    Book  Google Scholar 

  5. Burgess PJ, Rosati A. Advances in European agroforestry: results from the AGFORWARD project. Agrofor Syst. 2018;92(4):801–10. https://doi.org/10.1007/s10457-018-0261-3.

    Article  Google Scholar 

  6. Toussaint M, Darrot C. Enquête sociologique auprès des agriculteurs planteurs de bocage. nquête sociologique auprès des agriculteurs planteurs de bocage UMR CNRS 6(149 pp), 03277645, 2021

  7. Graham L, Gaulton R, Gerard F, Staley JT. The influence of hedgerow structural condition on wildlife habitat provision in farmed landscapes. Biol Conser. 2018;220:122–31. https://doi.org/10.1016/j.biocon.2018.02.017.

    Article  Google Scholar 

  8. Dover JW. The ecology of hedgerows and field margins. New York: Routledge; 2019. p. 291.

    Book  Google Scholar 

  9. Montgomery I, Caruso T, Reid N. Hedgerows as ecosystems: service delivery, management, and restoration. Annu Rev Ecol Evol Syst. 2020;51:81–102. https://doi.org/10.1146/annurev-ecolsys-012120-100346.

    Article  Google Scholar 

  10. Gotelli NJ, Colwell RK. Quantifying biodiversity: procedures and pitfalls in the measurement and comparison of species richness. Ecol Lett. 2001;4(4):379–91. https://doi.org/10.1046/j.1461-0248.2001.00230.x.

    Article  Google Scholar 

  11. Hallmann CA, Ssymank A, Sorg M, de Kroon H, Jongejans E. Insect biomass decline scaled to species diversity: general patterns derived from a hoverfly community. Proc Natl Acad Sci U S A. 2021;118(2):1–8. https://doi.org/10.1073/PNAS.2002554117.

    Article  Google Scholar 

  12. Gurevitch J, Koricheva J, Nakagawa S, Stewart G. Meta-analysis and the science of research synthesis. Nature. 2018;555(7695):175–82. https://doi.org/10.1038/nature25753.

    CAS  Article  PubMed  Google Scholar 

  13. Fraser H, Parker T, Nakagawa S, Barnett A, Fidler F. Questionable research practices in ecology and evolution. PLOS ONE. 2018;13(7):0200303. https://doi.org/10.1371/JOURNAL.PONE.0200303.

    Article  Google Scholar 

  14. Koricheva J, Gurevitch J, Mengersen K. Handbook of meta-analysis in ecology and evolution. Princeton University Press: Princeton; 2013. p. 498.

    Book  Google Scholar 

  15. Mupepele A-C, Walsh JC, Sutherland WJ, Dormann CF. An evidence assessment tool for ecosystem services and conservation studies. Ecol Appl. 2016;26(5):1295–301. https://doi.org/10.1890/15-0595.

    Article  PubMed  Google Scholar 

  16. Mupepele A-C, Dormann CF. Evidence ranking needs to reflect causality. Trends Ecol Evol. 2020;35(2):94–5. https://doi.org/10.1016/j.tree.2019.10.013.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

The first author was supported by the Ministry Of Science, Research and the Arts Baden-Württemberg, Germany. The article processing charge of the original article was funded by the Baden-Württemberg Ministry of Science, Research and Art and the University of Freiburg in the funding programme Open Access Publishing.

Author information

Authors and Affiliations

Authors

Contributions

ACM and CFD wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Anne-Christine Mupepele.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1.

Data for the meta-analysis on biodiversity.

Additional file 2.

Description of data tables and R-Code of the analysis.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Mupepele, AC., Dormann, C.F. Reply to: “Research on agroforestry systems and biodiversity conservation: what can we conclude so far and what should we improve?” by Boinot et al. 2022. BMC Ecol Evo 22, 65 (2022). https://doi.org/10.1186/s12862-022-02016-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12862-022-02016-7

Keywords

  • Species richness
  • Internal validity
  • External validity
  • Silvoarable
  • Silvopasture