Fig. 5
From: Extensive non-redundancy in a recently duplicated developmental gene family
Cuticle integrity of warthog mutants. a Depicts the fragile and perforated cuticles of wrt-3(ok2608);wrt-9(ok2732) mutants that was noticed upon the initial construction of these double mutant animals. b Shows the % penetrance of moulting defects (+ SD) in WT (n = 53), wrt-3(ok2608) (n = 53), wrt-9(ok2732) (n = 57) and wrt-3(ok2608);wrt-9(ok2732) (n = 56) animals, respectively. The mean % penetrance of moulting defects present in wrt-3(ok2608) single mutant and wrt-3(ok2608);wrt-9(ok2732) double mutant animals was compared with an unpaired t test and found not to be significant (nsP > 0.05). c–g Depict worms which have been soaked with DAPI for 15 min and imaged using 100 ms exposure time. h Depicts the quantification of DAPI fluorescence using a scoring system established in [35] using this DAPI assay where the x-axis is the % of total worms imaged. c–f Represent ‘Minimal’ fluorescence, while (g) represents ‘Bright’ fluorescence. The fluorescence observed in (c–f) is autofluorescence, rather than DAPI stain. Wild-type (n = 45), 97.44% minimal,wrt-5(ok670) (n = 51), 100% minimal; wrt-3(ok2608) (n = 55), 94.74% minimal; wrt-9(ok2732) (n = 55), 97.44% minimal; wrt-3(ok2608);wrt-9(ok2732) (n = 59), 95.00% bright. Scale bars 50 μm