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Table 3 P450 genotype and allele frequencies in a cold selection environment

From: The effect of three environmental conditions on the fitness of cytochrome P450 monooxygenase-mediated permethrin resistance in Culex pipiens quinquefasciatus

  

Genotype frequency

Observed (Expected)

Allele frequency

   

Cross

Gen.

SS

RS

RR

S

R

na

HWEb

Driftb

A #1

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.46 (0.53)

0.54 (0.39)

0 (0.08)

0.73

0.27

280

<0.0001*

<0.0001*

 

F8

0.26 (0.38)

0.71 (0.47)

0.03 (0.15)

0.62

0.38

920

<0.0001*

0.0066

 

F12

0.32 (0.44)

0.68 (0.45)

0 (0.11)

0.66

0.34

160

<0.0001*

0.1841

A #2

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.21 (0.21)

0.49 (0.49)

0.30 (0.30)

0.46

0.54

310

0.8320

0.1068

 

F8

0.04 (0.17)

0.73 (0.48)

0.23 (0.35)

0.41

0.59

1280

<0.0001*

0.1716

 

F12

0 (0.17)

0.84 (0.49)

0.16 (0.34)

0.42

0.58

1040

<0.0001*

0.3875

B #1

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.12 (0.22)

0.70 (0.50)

0.18 (0.28)

0.47

0.53

140

<0.0001*

0.2041

 

F8

0.07 (0.28)

0.86 (0.50)

0.04 (0.22)

0.53

0.47

1160

<0.0001*

0.1204

 

F12

0.27 (0.39)

0.71 (0.47)

0.02 (0.14)

0.63

0.37

560

<0.0001*

0.0284

B #2

F2

0.50

0.25

0.25

0.50

0.50

   
 

F4

0.45 (0.50)

0.52 (0.41)

0.03 (0.09)

0.71

0.29

270

<0.0001*

<0.0001*

 

F8

0.19 (0.27)

0.66 (0.50)

0.15 (0.23)

0.52

0.48

840

<0.0001*

<0.0001*

 

F12

0.23 (0.36)

0.74 (0.48)

0.03 (0.16)

0.60

0.40

800

<0.0001*

0.0529

  1. atotal number of 4th instar larvae used in genotype monitoring assay
  2. bNominal p-values are reported for the probability that the data fit the null hypothesis of Hardy-Weinberg equilibrium (or genetic drift)
  3. * indicates tests that remain statistically significant after Bonferonni correction over the entire experiment