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Table 1 P450 genotype and allele frequencies in a standard laboratory environment

From: The effect of three environmental conditions on the fitness of cytochrome P450 monooxygenase-mediated permethrin resistance in Culex pipiens quinquefasciatus

  

Genotype frequency

Observed (Expected)

Allele Frequency

   

Cross

Gen.

SS

RS

RR

S

R

na

HWEb

Driftb

A #1

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.69 (0.69)

0.28 (0.28)

0.03 (0.03)

0.83

0.17

120

0.9203

<0.0001*

 

F5

0.31 (0.38)

0.62 (0.47)

0.07 (0.15)

0.62

0.38

960

<0.0001*

<0.0001*

 

F6#

0.31 (0.34)

0.55 (0.49)

0.14 (0.17)

0.59

0.41

1040

<0.0001*

0.0105

 

F7#

0.24 (0.33)

0.67 (0.49)

0.09 (0.18)

0.58

0.42

1560

<0.0001*

0.2039

 

F8

0.19 (0.27)

0.66 (0.50)

0.15 (0.23)

0.52

0.48

900

<0.0001*

0.0004*

 

F9#

0.17 (0.24)

0.63 (0.50)

0.20 (0.26)

0.49

0.51

1640

<0.0001*

0.0023

 

F10#

0.21 (0.30)

0.68 (0.50)

0.11 (0.20)

0.55

0.45

1760

<0.0001*

1

 

F11

0.24 (0.35)

0.70 (0.48)

0.06 (0.17)

0.59

0.41

1520

<0.0001*

0.0009*

 

F12

0.47 (0.46)

0.41 (0.43)

0.12 (0.11)

0.68

0.32

400

0.19

0.0002*

A #2

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.35 (0.43)

0.61 (0.45)

0.04 (0.12)

0.66

0.34

80

0.0018

0.0024

 

F5

0.44 (0.46)

0.48 (0.44)

0.08 (0.10)

0.68

0.32

420

0.0349

0.1427

 

F6

0.76 (0.77)

0.23 (0.22)

0.01 (0.01)

0.88

0.12

1200

0.0748

<0.0001*

 

F7

0.77 (0.77)

0.22 (0.22)

0.01 (0.01)

0.88

0.12

1440

0.1138

0.2772

 

F8

0.79 (0.79)

0.20 (0.20)

0.01 (0.01)

0.89

0.11

900

0.5199

<0.0001*

 

F9

0.83 (0.83)

0.17 (0.16)

0 (0.01)

0.92

0.08

560

0.0279

0.0252

 

F10

0.78 (0.78)

0.21 (0.21)

0.01 (0.01)

0.89

0.11

480

0.4884

<0.0001*

 

F11

0.36 (0.36)

0.49 (0.48)

0.15 (0.16)

0.61

0.39

520

0.5651

<0.0001*

 

F12

0.38 (0.38)

0.48 (0.48)

0.14 (0.14)

0.62

0.38

240

0.7719

0.3329

B #1

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.35 (0.44)

0.62 (0.45)

0.03 (0.11)

0.66

0.34

160

<0.0001*

<0.0001*

 

F5#

0.25 (0.36)

0.70 (0.48)

0.05 (0.16)

0.60

0.40

320

<0.0001*

0.0144

 

F6#

0.13 (0.19)

0.61 (0.49)

0.26 (0.32)

0.44

0.56

760

<0.0001*

1

 

F7

0.19 (0.29)

0.69 (0.50)

0.12 (0.21)

0.54

0.46

1440

<0.0001*

<0.0001*

 

F8#

0.22 (0.29)

0.64 (0.50)

0.14 (0.21)

0.54

0.46

800

<0.0001*

0.4023

 

F9#

0.13 (0.27)

0.77 (0.50)

0.10 (0.23)

0.52

0.48

1640

<0.0001*

0.0209

 

F10#

0.24 (0.35)

0.71 (0.48)

0.05 (0.16)

0.60

0.40

680

<0.0001*

0.1192

 

F11

0.73 (0.75)

0.27 (0.23)

0 (0.02)

0.87

0.13

680

<0.0001*

<0.0001*

 

F12#

0.64 (0.66)

0.35 (0.30)

0.01 (0.04)

0.82

0.18

880

<0.0001*

0.9999

B #2

F2

0.25

0.50

0.25

0.50

0.50

   
 

F4

0.36 (0.46)

0.63 (0.44)

0.01 (0.10)

0.68

0.32

300

<0.0001*

<0.0001*

 

F5

0.15 (0.27)

0.74 (0.50)

0.11 (0.23)

0.52

0.48

880

<0.0001*

<0.0001*

 

F6#

0.30 (0.37)

0.62 (0.48)

0.08 (0.15)

0.61

0.39

1440

<0.0001*

1

 

F7

0.18 (0.30)

0.74 (0.50)

0.08 (0.20)

0.55

0.45

1440

<0.0001*

<0.0001*

 

F8

0.47 (0.50)

0.48 (0.42)

0.05 (0.08)

0.71

0.29

520

0.0002*

<0.0001*

 

F9

0.38 (0.40)

0.50 (0.47)

0.12 (0.13)

0.63

0.37

920

0.0279

<0.0001*

 

F10#

0.50 (0.53)

0.45 (0.40)

0.05 (0.07)

0.73

0.27

1800

<0.0001*

1

 

F11

0.67 (0.70)

0.33 (0.28)

0 (0.02)

0.84

0.16

1000

<0.0001*

<0.0001*

 

F12

0.50 (0.56)

0.50 (0.38)

0 (0.06)

0.75

0.25

1700

<0.0001*

<0.0001*

  1. atotal number of 4th instar larvae used in genotype monitoring assay
  2. bNominal p-values are reported for the probability that the data fit the null hypothesis of Hardy-Weinberg equilibrium (or genetic drift)
  3. * indicates tests that remain statistically significant after Bonferonni correction over the entire experiment
  4. # indicates generations that are out of Hardy-Weinberg equilibrium likely due to genetic drift