- Research article
- Open Access
Nuptial gifts fail to resolve a sexual conflict in an insect
BMC Evolutionary Biology volume 8, Article number: 204 (2008)
Because of the potential benefits to individuals of saving investment for future mating opportunities, there is conflict between mates over most aspects of reproduction. Males of many species transfer compounds in the ejaculate that manipulate female reproductive physiology to increase male reproductive success. These seminal compounds are often associated with direct and/or indirect costs to females. In contrast, in some species ejaculates also contain nutrients used by females for somatic maintenance and increased reproductive output. In general, the extent to which male seminal components are detrimental or beneficial to females is poorly understood, and interactions between seminal compounds with different effects have been almost completely neglected. Here we examine the impact of male receptivity-suppressing factors and nutrient donations on female longevity and lifetime reproductive output in the bushcricket Requena verticalis.
We show that receiving multiple ejaculates reduces longevity in female R. verticalis, indicating a cost of male derived receptivity-suppressing compounds. Consumption of male nutrient donations does not appear to ameliorate this longevity cost, and there was no effect of nutrient provisioning on female lifetime fecundity.
These results indicate that nutrient provisioning does not provide a resolution to sexual conflict over female receptivity in this bushcricket species.
The reproductive interests of mates rarely coincide, resulting in sexual conflict over most aspects of reproduction [1, 2]. There is frequent conflict over female remating, as males will have to endure sperm competition and reduced fertilization success when females mate again . The risk of sperm competition has promoted a variety of male adaptations, often associated with female costs . Males frequently transfer compounds in the ejaculate that manipulate female reproductive physiology to increase male reproductive success. For example, Drosophila melanogaster males transfer a cocktail of >80 different proteins in the ejaculate that (amongst other things) stimulate oviposition and reduce female receptivity, thereby increasing male fertilization success [5, 6]. However, these male-derived molecules have a negative effect on female fitness by reducing lifespan . It has even been suggested that male harm could evolve as a means to manipulate females to increase their terminal investment in immediate reproductive output, due to reduced residual reproductive value, which then results in higher male reproductive success [8, 9].
Not all compounds transferred to the female at mating have a negative effect on female fitness. Males of several insects transfer nutrients at mating, either in the ejaculate or together with the sperm packet, that increase female reproductive success by enhancing fecundity and/or offspring survival [10–13]. As a consequence, male nutrient donations create an additional conflict over mating; a female should remate and obtain additional nutrients to increase her fecundity, whereas a male should prevent the female from remating to ensure paternity of the offspring in which he invests . For example, in Pieris napi butterflies, male spermatophores contain nutrients increasing both female fecundity and longevity , and anti-aphrodisiacs that render females unattractive to rival males following mating , as well as large numbers of non-fertile sperm that switch off long-term female receptivity . Nutrient donations can represent a substantial investment by male insects, at times approaching or even exceeding that of female investment in egg production [18, 19], and require extended periods of male recouperation [20, 21]. It is therefore likely that the greater the value to females of receiving male donations together with the costs to males of providing such gift, will exacerbate the level of sexual conflict over female remating rate.
In species where the cost of providing nutrients directly limits male mating rate, there is particularly strong selection on males to reduce female remating and the ensuing sperm competition, and opposing selection on females to increase their mating rate. Male insects have evolved a variety of ways to reduce female remating, ranging from mate guarding and physical barriers preventing additional copulations, to transfer of various receptivity suppressing ejaculate components, including large numbers of non-fertile sperm [5, 22–24]. These adaptations are known to be effective in reducing female remating rates, even if not successfully preventing female remating altogether . Some of these male adaptations are known to impose costs on females , which may also be borne by the manipulating male, if production of his offspring is reduced. This creates a somewhat paradoxical situation in which males compromise their own fitness by harming their mates, a paradox which is normally explained by the potential for the benefits to males from reduced female remating to exceed the costs from reduced fecundity. However, if males reduce female fecundity below its maximum, this creates selection for a male adaptation that restores female fecundity, which provides a resolution to this paradox.
Males of many species of bushcrickets (Orthoptera: Tettigoniidae) provide females with a large nuptial gift, synthesized by the male at mating, that increase female fecundity and offspring fitness [10, 13, 25]. Nutrient provisioning is often associated with male mating costs in terms of time required before being able to produce a new spermatophore and mate again [20, 21, 26]. The spermatophore of the univoltine Australian bushcricket Requena verticalis is, like most bushcricket species, comprised of a sperm-containing ampulla and a sperm-free gelatinous spermatophylax that contains male-derived nutrients that can directly enhance female fecundity . The entire spermatophore is attached externally to the females' genital opening at mating. The female removes and feeds on the sperm-free spermatophylax during sperm transfer from the ampulla, and will later remove and consume the empty ampulla. The spermatophylax in R. verticalis therefore serves a dual role of both protecting the ejaculate during insemination, and as a paternal investment . Males incur a substantial cost of spermatophore production requiring several days before being able to mate again . Following mating the female enters into a non-receptive period, which is directly related to the amount of ejaculate transferred . However, the duration of a female's non-receptivity period depends on her nutritional status , suggesting substantial sexual conflict over female remating rate .
Previous work has shown nutrients provided in the spermatophylax increase immediate female fecundity, egg weight and offspring survival [25, 32]. However, the impact of spermatophylax consumption and amount of ejaculate received on female lifetime fecundity or longevity has not been examined. This is despite the frequently observed negative impact on female lifespan of receptivity-suppressing compounds transferred in the ejaculate of several insect taxa . To examine the hypothesis that potentially costly manipulative ejaculates can be compensated for by simultaneous provisioning of nutrients, we examine the impact of varying the amount of ejaculate received, and spermatophylax consumption on female lifespan and lifetime reproductive output. We specifically ask whether male spermatophylax provisioning can compensate for the potential cost to females of receiving manipulative ejaculates, which may indicate that males provide nutrient to restore female fecundity, whilst simultaneously enjoying high paternity by reducing the risk of sperm competition. We show that ejaculate receipt is costly to females in terms of reduced longevity, and that spermatophylax consumption does not appear to ameliorate this cost.
Female lifespan ranged between 36 – 147 days post first copulation, and differed with respect to mating treatment. There was a significant effect of our mating treatments on female lifespan (χ2 = 6.59, p = 0.037, Fig 1), but no effect of either female size (χ2 = 1.49, p > 0.2) or lifetime fecundity (χ2 = 0.47, p > 0.4). Planned comparison of the impact of the amount of ejaculate received on longevity revealed a cost of ejaculate receipt, with females receiving three full ejaculates dying sooner than females receiving one ejaculate (χ2 = 4.54, p = 0.033), and no effect of either female size (χ2 = 0.083, p > 0.7) or lifetime fecundity (χ2 = 0.15, p > 0.6) on lifespan. However, the planned comparison examining the impact of spermatophylax consumption showed there was no difference in longevity between females receiving one ejaculate with respect to consuming spermatophylax material or not (χ2 = 1.29, p > 0.2), with again no influence of female size (χ2 = 0.74, p > 0.3) or lifetime fecundity (χ2 = 0.001, p > 0.9). On average, females receiving three ejaculates lived 75 days compared to 84 and 84.5 days for females receiving one ejaculate only, or one ejaculate and allowed to consume three spermatophylaces, respectively. The cost in terms of reduced lifespan of receiving three ejaculates versus one is ~11 days, whereas the (non-significant) positive effect of consuming three spermatophylaces versus none (when receiving one ejaculate) is only ~ half a day. This clearly indicates a cost to females of receiving male ejaculates in terms of reduced longevity, and no impact on lifespan of spermatophylax consumption. We therefore conclude that spermatophylax consumption do not ameliorate the cost of receiving multiple ejaculates in R. verticalis.
To clarify that any potential fecundity effects were not confounded by differences in longevity between treatments, we examined the impact of mating treatment across all females. There was no difference between mating treatments with respect to female lifetime fecundity, mean egg weight, the total mass of eggs produced, or egg-laying rate (Table 1, Fig 2). Although bigger females had higher total reproductive output (total mass of eggs laid: F1,41 = 4.68, p = 0.037) they did not lay heavier eggs (F1,41 = 2.69, p > 0.11). Across all females there was no relationship between female longevity and fecundity (F1,41 = 0.30, p > 0.58), mean egg weight (F1,41 = 0.004, p > 0.95), or between longevity and female size (F1,41 = 0.51, p > 0.48). Mating treatment did also not affect the onset of egg-laying following mating (Likelihood ratio χ2 = 9.34, p > 0.16).
Our results show that receiving multiple copulations and/or receiving multiple ejaculates reduces female R. verticalis lifespan, and that consumption of male nutrient donations cannot ameliorate this cost. Our experimental design cannot separate possible effects on female lifespan of multiple copulations from those of ejaculate compounds. However, matings do not appear to involve any behaviour that might directly impose costs on females, at least in the laboratory where predation is absent. Males attach the spermatophore externally to the females' genital opening; hence insemination occurs unaided by any male intromittent organ. Previous work in R. verticalis demonstrates that the ejaculate contains factors that suppress female receptivity, and that this effect is directly related to the amount of ejaculate received . Similar findings come from studies of a number of other bushcricket species, indicating that the ejaculate in general contains receptivity-suppressing compounds in this insect family [33–35]. In general, receptivity-suppressing compounds are transferred to the female at mating in several insects [e.g. [7, 22, 24, 36]]. It is not clear what these compounds are, but in gryllid crickets prostaglandins or prostaglandin precursors are transferred in the seminal fluid to females where they trigger oviposition [33, 37–40]. Male gryllid crickets also transfer > 30 different additional seminal proteins, which show evidence of rapid evolution indicative of ongoing selection, although their function in regulating female reproduction is, as yet, unclear . In contrast to the marked effects of ejaculates, there is no evidence that spermatophylax consumption has any effect on female R. verticalis receptivity .
Previous work has shown that female R. verticalis incorporate male derived nutrients passed in the spermatophylax into their soma, and that they retain a larger amount of male nutrients when experiencing nutritional stress . However, our results found no evidence that females make use of nutrients in the spermatophylax for their somatic maintenance to thereby ameliorate the longevity cost of receiving ejaculates. This suggests that male derived nutrients are not important to female fitness in terms of increasing lifespan. The importance of male derived nutrients to female fitness has previously been demonstrated in the bushcricket Kawanaphila nartee, where the duration of the female's refractory period is dependent not only on the amount of ejaculate received, but also on their diet, with females in greater nutritional need having shorter periods of non-receptivity . Females appear to overcome male manipulation when the value of males' nutrient donations is large. The same may be true for R. verticalis, since poorly nourished females have a shorter refractory period than well-fed females , although it is not clear from our results that this carries any direct benefits in terms of increased longevity or lifetime fecundity. It is possible that the low protein diet females were kept on reduced their ability overall to produce eggs relative to females in the field, where presumably they encounter a more varied diet and hence a longer lifespan may translate to increased egg production.
In other insects, females do make use of male derived nutrients to extend their lifespan . For example, in the green-veined white butterfly (P. napi), male derived nutrients are important to female fitness not only by increasing fecundity, but also by allowing old females to histolyse their wing muscles and convert these resources into eggs . Male nutrient provisioning therefore ensures females live sufficiently long to have time to convert their wing muscles into more eggs. Similarly, in the comma butterfly (Polygonia c-album), male-derived nutrients allow females to increase their reproductive output without associated longevity costs since they can make use of male donations for their somatic maintenance . In some gryllid crickets, female multiple mating is also associated with enhanced longevity, although it is not clear if this is directly due to benefits from spermatophore consumption, as the effect was observed in species lacking a nutritious spermatophylax . Similarly, in the cricket Gryllus lineaticeps, males do not provide a spermatophylax, yet females mating multiply to preferred males enjoy both increased longevity and fertility, which is likely due to variation in seminal fluid quality between males .
Contrary to previous work [e.g. [25, 32]], we did not find that females allowed to consume three spermatophylaces laid more or heavier eggs in their lifetime than females prevented from spermatophylax consumption. To examine whether the lack of observed difference in female reproductive output could be explained by low power in our experiment relative to previous published results [e.g. ], we examined the effect sizes of spermatophylax feeding on egg weight as this measure is comparable in the two studies [46, 47]. The sample sizes of the experiments are of similar magnitude (12–14 versus 11–16, our study), and the effect size in our study was roughly half (r = 0.142) that of Gwynne's  (r = 0.316). Overall, eggs were heavier in our study compared to those in Gwynne  (range 2.15 – 2.29 versus 1.94 – 2.06 mg), but were similar in weight to that reported by Gwynne and co-workers  (2.15 – 2.19 mg), indicating substantial variation among females in this trait. Previous work has shown that adult diet can directly influence female fecundity, but not egg weight [32, 42], so that the effect of spermatophylax consumption on female fecundity may depend on adult female diet. One possible explanation for the difference in the effect of spermatophylax consumption between the studies therefore, is that we did not restrict the diet sufficiently for the females in our study. We think this unlikely as we used the same low quality adult diet, composed solely of rolled oats and water, as that used by Gwynne , who also showed that the effect of spermatophylax consumption on fecundity was not dependent upon adult diet manipulation. Although we cannot rule out there may be subtle differences in the nutrient composition of the diet between the experiments that may affect female fecundity, this cannot explain the absence of an effect of spermatophylax consumption in out study. Additionally, here we measured female lifetime fecundity and egg production using a mating frequency characteristic of natural populations , whereas previous work examined the effect of spermatophylax consumption on female fecundity and egg weight over a much shorter time scale (~first 30 days after mating). A further reason for the differences may be that Gwynne  used shorter remating intervals than females experienced in this study, which were chosen to reflect the mating frequency observed in natural populations . It would thus appear that the main function of the spermatophylax in this species is to protect the ejaculate during insemination, but that it may also serve to provide nutrients to females during some circumstances .
It is not clear why females of some species are able to retain some control over their reproduction whereas other species seem to be at the mercy of manipulative males. Sexual conflict generates continuous adaptation and counter-adaptation of reproductive traits by the sexes [50–52], which can lead to rapid elaboration of traits important to reproductive success . For example, there is evidence that male seminal products are under strong positive selection, and are rapidly evolving in both insects and vertebrates, including man [53–55]. The reason for the rapid evolution of seminal traits is thought to be that males are selected to continuously evolve more potent compounds, as females evolve increased resistance to male manipulation . At any point in time, either males or females may have the 'upper hand', which may in part explain the observed differences between species in the extent of female control. In addition, the value and cost of female remating varies between species, which could influence the outcome. It has been proposed that females may gain the upper hand in sexual conflict when the value of 'winning' is greater and the cost of resisting manipulative males is low . Our results suggest that the longevity cost to R. verticalis females associated with male induced non-receptivity does not compromise overall female fitness, because it does not appear to affect lifetime fecundity. It is unlikely that females could benefit from reduced lifespan via a reduction in generation time, as R. verticalis is univoltine, having an obligate overwinter diapause. The benefits to males of inducing non-receptivity periods may thus outweigh the longevity costs to females, so that manipulative ejaculates can be maintained.
We demonstrate that male nutrient donations do not ameliorate the longevity costs to R. verticalis females of receiving ejaculates with receptivity-suppressing compounds, or provide a direct benefit by increasing their lifetime egg production. Nutrient provisioning does not appear to provide a resolution to sexual conflict over female receptivity in this bushcricket. Our results suggest that substantial conflicts of interest over mating rate can persist, and that matings can continue to be harmful to females even when they involve substantial investment by males.
Female R. verticalis were collected as last instar nymphs from around the campus of the University of Western Australia. Males were caught either as last instar nymphs or adults. Insects were kept in individual Perspex vials, 6.5 cm in diameter and 15 cm tall. Females were kept on a low protein diet consisting of rolled oats and water. This diet was chosen to maximize the effect of our mating treatment, and should reflect situations of low protein availability in the field. Males were also fed dried cat food as a protein source to ensure they were capable of producing a large spermatophore and associated nutritious spermatophylax . Adult males were kept in the lab in this way for at least a week before being used as mating partners for our experimental females. Experimental females were at least 5 days post adult eclosion before being offered a male for their first copulation (range 5–9 days). Each female was placed into the vial of a singing male. If the female did not show signs of receptivity within 3 hours or attempted to cannibalise the male, she was removed from the vial and presented with a new male the following day until she mated for the first time.
Females were allocated randomly to one of three mating treatments. Females in the first treatment received three ejaculates but no spermatophylax meals. Thus, females were allowed to mate on three occasions separated by 6–10 days. This remating interval was chosen to conform to the natural lifetime mating frequency of field-collected females . Following mating the spermatophylax was removed from the ampulla of the spermatophore with forceps, while ensuring the ampulla remained attached to the female's genital opening. The female was then placed in a narrow tube to prevent her from removing the ampulla. Females were kept in the tube for 190 min following ampulla attachment to ensure complete transfer of the ejaculate . Females in the second treatment received a single ejaculate at their first mating and were also prevented from consuming their spermatophylax. They received no further matings. Females in the third treatment also received a single mating, but they were allowed to consume their spermatophylax. These females received two additional spermatophylaces at 6–10 day intervals. Thus, females received either (1) three ejaculates no spermatophylax; (2) one ejaculate no spermatophylax; or (3) one ejaculate and three spermatophylaces. The females in treatments 2 and 3 were subjected to 190 min sessions in the narrow tube to control for any potential effect that tube exposure may have had on females in treatment 1. All matings were conducted under red light at 28°C.
Following the mating treatment, females were returned to their individual vials, and provided with damp sand as egg laying substrate. The sand was sieved on three occasions at 5 day intervals. The first egg counting interval included the eggs laid by the female in the first 5 days since completion of their mating treatment, and any eggs laid during the time of the mating treatment. Eggs were counted, and weighed to the nearest 0.01 mg. Females were then monitored daily until they died, and all the eggs laid since the last egg check were counted and weighed as before. Lifespan was measured as the number of days since their first copulation until death. Females that laid fewer than 10 eggs during the their lifespan (N = 6) were excluded from the analyses. At death the pronotum width was measured as an estimate of female body size. Overall, there was no difference in female body size with respect to mating treatment (F = 0.15, p > 0.8, N = 43).
The effect of mating treatment on female longevity was examined using Cox's proportional hazards analyses on uncensored data, as we have known lifespan for all females, with female size and lifetime fecundity as covariates. We conducted two planned contrasts. The effect of mating on longevity was examined by contrasting the longevity of females receiving 1 versus 3 ejaculates. The effect of spermatophylax feeding on longevity was determined by contrasting the longevity of females that received a single ejaculate without spermatophylax feeding or given access to three spermatophylaces.
The effect of mating treatment on female reproductive output was examined by looking for differences in lifetime fecundity, the number of eggs laid in each of the four egg counting periods (i.e. 5, 10, 15 days or after mating or between day 16 and until death), egg weight (including egg weight in each of the four egg counting periods), and egg laying rate in each of the four periods. We used Generalized Linear Models specifying a Poisson error distribution (data corrected for over-dispersion) with female pronotum size as a covariate where appropriate. The effect of mating treatment on onset of egg laying was analysed using contingency test. Data analyses were performed in R version 2.2.1 .
Arnqvist G, Rowe L: Sexual Conflict. 2005, Princeton, Princeton University Press
Wedell N, Kvarnemo C, Lessells CM, Tregenza T: Sexual conflict and life histories. Anim Behav. 2006, 71: 999-1011. 10.1016/j.anbehav.2005.06.023.
Parker GA: Sperm competition and its evolutionary consequences in insects. Biol Rev. 1970, 45: 525-567. 10.1111/j.1469-185X.1970.tb01176.x.
Stockley P: Sexual conflict resulting from adaptations to sperm competition. Trends Ecol Evol. 1997, 12: 154-159. 10.1016/S0169-5347(97)01000-8.
Chapman T: Seminal fluid-mediated fitness traits in Drosophila. Heredity. 2001, 87: 511-521. 10.1046/j.1365-2540.2001.00961.x.
Wolfner MF: The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila. Heredity. 2002, 88: 85-93. 10.1038/sj.hdy.6800017.
Chapman T, Liddle LF, Kalb JM, Wolfner MF, Partridge L: Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products. Nature. 1995, 373: 241-244. 10.1038/373241a0.
Johnstone RA, Keller L: How males can gain by harming their mates: sexual conflict, seminal toxins, and the cost of mating. Am Nat. 2000, 156: 368-377. 10.1086/303392.
Lessells CM: Why are males bad for females? Models for the evolution of damaging male mating behaviour. Am Nat. 2005, 165: S46-S63. 10.1086/429356.
Vahed K: The function of nuptial feeding in insects: a review of empirical studies. Biol Rev. 1998, 73: 43-78. 10.1017/S0006323197005112.
Arnqvist G, Nilsson T: The evolution of polyandry: multiple mating and female fitness in insects. Anim Behav. 2000, 60: 145-164. 10.1006/anbe.2000.1446.
Vahed K: All that glisters is not gold: sensory bias, sexual conflict and nuptial feeding in insects and spiders. Ethology. 2007, 113: 105-127. 10.1111/j.1439-0310.2006.01312.x.
Gwynne DT: Sexual conflict over nuptial gifts in insects. Ann Rev Entomol. 2008, 53: 83-101. 10.1146/annurev.ento.53.103106.093423.
Simmons LW, Parker GA: Nuptial feeding in insects: mating effort versus paternal investment. Ethology. 1989, 81: 332-343.
Wiklund C, Kaitala A, Wedell N: Decoupling of reproductive rates and parental expenditure in a polyandrous butterfly. Behav Ecol. 1998, 9: 20-25. 10.1093/beheco/9.1.20.
Andersson J, Borg-Karlson AK, Wiklund C: Sexual cooperation and conflict in butterflies: A male-transferred anti-aphrodisiac reduces harassment of recently mated females. Proc R Soc Lond B. 2000, 267: 1271-1275. 10.1098/rspb.2000.1138.
Cook PA, Wedell N: Non-fertile sperm delay female remating. Nature. 1999, 397: 486-10.1038/17257.
Simmons LW: Quantification of role reversal in relative parental investment in a bush cricket. Nature. 1992, 358: 61-63. 10.1038/358061a0.
Wedell N, Karlsson B: Paternal investment directly affects female reproductive effort in an insect. Proc R Soc Lond B. 2003, 270: 2065-2071. 10.1098/rspb.2003.2479.
Wedell N: Dual function of the bush cricket spermatophore. Proc R Soc Lond B. 1994, 258: 181-185. 10.1098/rspb.1994.0160.
Vahed K: Comparative evidence for a cost to males of manipulating females in bushcrickets. Behav Ecol. 2007, 18: 499-506. 10.1093/beheco/arm021.
Gillott C: Male accessory gland secretions: Modulators of female reproductive physiology and behavior. Ann Rev Entomol. 2003, 48: 163-184. 10.1146/annurev.ento.48.091801.112657.
Till-Bottraud I, Joly D, Lachaise D, Snook RR: Pollen and sperm heteromorphism: convergence across kingdoms?. J Evol Biol. 2004, 18: 1-18. 10.1111/j.1420-9101.2004.00789.x.
Wedell N: Female receptivity in butterflies and moths. J Exp Biol. 2005, 208: 3433-3440. 10.1242/jeb.01774.
Gwynne DT: Courtship feeding increases female reproductive success in bushcrickets. Nature. 1984, 307: 361-363. 10.1038/307361a0.
Simmons LW: Nuptial feeding in tettigoniids: male costs and the rates of fecundity increase. Behav Ecol Sociobiol. 1990, 27: 43-47. 10.1007/BF00183312.
Simmons LW: Courtship feeding in katydids (Orthoptera: Tettigoniidae): investment in offspring and in obtaining fertilizations. Am Nat. 1995, 146: 307-315. 10.1086/285801.
Simmons LW: Some constraints on reproduction for male bush-crickets, Requena verticalis (Orthoptera: Tettigoniidae) – diet, size and parasite load. Behav Ecol Sociobiol. 1993, 32: 135-139. 10.1007/BF00164046.
Gwynne DT: Courtship feeding in katydids: investment in offspring or in obtaining fertilizations? (Orthoptera: Tettigoniidae). Am Nat. 1986, 128: 342-352. 10.1086/284566.
Gwynne DT: Testing parental investment and the control of sexual selection in katydids: the operational sex ratio. Am Nat. 1990, 136: 474-484. 10.1086/285108.
Simmons LW, Gwynne DT: The refractory period of female katydids: sexual conflict over the remating interval?. Behav Ecol. 1991, 2: 276-282. 10.1093/beheco/2.4.276.
Gwynne DT: Courtship feeding and the fitness of female katydid (Orthoptera: Tettigoniidae). Evolution. 1988, 42: 545-555. 10.2307/2409038.
Destephano DB, Brady UE, Lovins RE: Synthesis of prostaglandin by reproductive tissue of the male house cricket, Acheta domesticus. Prostaglandins. 1974, 6: 71-9. 10.1016/S0090-6980(74)80042-0.
Wedell N, Arak A: The wartbiter spermatophore and its effect on female reproductive output (Orthoptera: Tettigoniidae, Decticus verrucivorus). Behav Ecol Sociobiol. 1989, 24: 117-125. 10.1007/BF00299643.
Bateman PW: Changes in phonotactic behaviour of a bushcricket with mating history. J Insect Behav. 2001, 14: 333-343. 10.1023/A:1011167128430.
Simmons LW: Sperm Competition and Its Evolutionary Consequences in Insects. 2001, Princeton, Princeton University Press
Destephano DB, Brady UE: Prostaglandin and prostaglandin-synthetase in the cricket Acheta domesticus. J Insect Physiol. 1977, 23: 905-11. 10.1016/0022-1910(77)90019-1.
Loher W, Ganjian WI, Kubo I, Stanley-Samuelson D, Tobe S: Prostaglandins: their role in egg laying in the cricket Teleogryllus commodus. Proc Natl Acad Sci USA. 1981, 78: 7835-8. 10.1073/pnas.78.12.7835.
Stanley-Samuelson DW, Loher W: Arachidonic and other long-chain polyunsaturated fatty acids in spermatophores and spermathecae of Teleogryllus commodus: significance in prostaglandin-mediated reproductive behaviour. J Insect Physiol. 1983, 29: 41-5. 10.1016/0022-1910(83)90104-X.
Stanley-Samuelson DW, Loher W: Prostaglandins in insect reproduction. Ann Entomol Soc Am. 1986, 79: 841-53.
Andrés JA, Maroja LS, Bogdanowicz SM, Swanson WJ, Harrison RG: Molecular evolution of seminal proteins in field crickets. Mol Biol Evol. 2006, 23: 1574-1584. 10.1093/molbev/msl020.
Gwynne DT, Brown WD: Mate feeding, offspring investment, and sexual differences in katydids (Orthoptera: Tettigoniidae). Behav Ecol. 1994, 5: 267-272. 10.1093/beheco/5.3.267.
Karlsson B: Nuptial gifts, resource budgets, and reproductive output in a polyandrous butterfly. Ecology. 1998, 78: 2931-2940.
Burpee DM, Sakaluk SK: Repeated matings offset costs of reproduction in female crickets. Evol Ecol. 1993, 7: 240-250. 10.1007/BF01237742.
Wagner WE, Harper CJ: Female lifespan and fertility are increased by the ejaculates of preferred males. Evolution. 2003, 57: 2054-2066.
Cohen J: Statistical Power Analysis for the Behavioral Sciences. 1988, Hillsdale, NJ, Lawrence Erlbaum Associates, 2
Rosenthal R: Meta-Analytic Procedures for Social Research (revised edition). 1991, London, Sage Publications
Gwynne DT, Bowen BJ, Codd CG: The function of the katydid spermatophore and its role in fecundity and insemination (Orthoptera: Tettigoniidae). Aust J Zool. 1984, 32: 15-22. 10.1071/ZO9840015.
Simmons LW, Beveridge M, Kennington WJ: Polyandry in the wild: temporal changes in female mating frequency and sperm competition intensity in natural populations of the tettigoniid Requena verticalis. Mol Ecol. 2007, 16: 4613-4623. 10.1111/j.1365-294X.2007.03528.x.
Rice WR: Sexually antagonistic male adaptation triggered by experimental arrest of female evolution. Nature. 1996, 381: 232-234. 10.1038/381232a0.
Chapman T: Evolutionary conflicts of interest between male and females. Curr Biol. 2006, 16: R744-R754. 10.1016/j.cub.2006.08.020.
Lessells CM: The evolutionary outcome of sexual conflict. Phil Trans R Soc Lond B. 2006, 361: 301-317. 10.1098/rstb.2005.1795.
Wyckoff GJ, Wang W, Wu C-I: Rapid evolution of male reproductive genes in the descent of man. Nature. 2000, 403: 304-309. 10.1038/35002070.
Swanson WJ, Vacquier VD: The evolution of reproductive proteins. Nature Rev Genet. 2002, 3: 137-144. 10.1038/nrg733.
Dorus S, Evans PD, Wyckoff GJ, Choi SS, Lahn BT: Rate of molecular evolution of the seminal protein gene SEMG2 correlates with levels of female promiscuity. Nat Genet. 2004, 36: 1326-1329. 10.1038/ng1471.
Parker GA, Partridge L: Sexual conflict and speciation. Phil Trans R Soc Lond B. 1998, 353: 261-274. 10.1098/rstb.1998.0208.
Ihaka R, Gentleman R: R: A language for data analysis and graphics. J Comp Graph Stat. 1996, 5: 299-314. 10.2307/1390807.
We thank Darryl Gwynne and two anonymous referees for helpful comments on this MS. This work was supported by Fellowships and international short visit grants from the Royal Society to NW and TT, and the Australian Research Council to LWS.
NW, TT, and LWS contributed to the conceptual development of the work, the experimental work and the writing of this manuscript. NW performed data analyses.
About this article
Cite this article
Wedell, N., Tregenza, T. & Simmons, L.W. Nuptial gifts fail to resolve a sexual conflict in an insect. BMC Evol Biol 8, 204 (2008). https://doi.org/10.1186/1471-2148-8-204
- Mating Treatment
- Sperm Competition
- Female Fecundity
- Sexual Conflict
- Female Fitness