- Research article
- Open Access
Barriers to gene exchange in hybridizing field crickets: the role of male courtship effort and cuticular hydrocarbons
BMC Evolutionary Biology volume 14, Article number: 65 (2014)
Pre-zygotic barriers often involve some form of sexual selection, usually interpreted as female choice, as females are typically the choosier sex. However, males typically show some mate preferences, which are increasingly reported. Here we document previously uncharacterized male courtship behavior (effort and song) and cuticular hydrocarbon (CHC) profiles in the hybridizing crickets Gryllus firmus and G. pennsylvanicus. These two species exhibit multiple barriers to gene exchange that act throughout their life history, including a behavioral barrier that results in increased time to mate in heterospecific pairs.
We demonstrated that male mate choice (as courtship effort allocation) plays a more important role in the prezygotic behavioral barrier than previously recognized. In gryllids females ultimately decide whether or not to mate, yet we found males were selective by regulating courtship effort intensity toward the preferred (conspecific) females. Females were also selective by mating with more intensely courting males, which happened to be conspecifics. We report no differences in courtship song between the two species and suggest that the mechanism that allows males to act differentially towards conspecific and heterospecific females is the cuticular hydrocarbon (CHC) composition. CHC profiles differed between males and females of both species, and there were clear differences in CHC composition between female G. firmus and G. pennsylvanicus but not between the males of each species.
Although many barriers to gene exchange are known in this system, the mechanism behind the mate recognition leading to reduced heterospecific mating remains unknown. The CHC profiles might be the phenotypic cue that allow males to identify conspecifics and thus to adjust their courtship intensity accordingly, leading to differential mating between species.
Pre-zygotic barriers to gene exchange can play a large role in reducing gene flow between species; these barriers act earlier in the life cycle and have the potential to restrict hybridization more than later acting barriers . Many of these barriers involve some form of sexual selection, usually interpreted as female choice, as females typically invest the most in the offspring  and thus tend to be choosier. However, male mate choice has now been described even in species where males do not invest in offspring care [3–6]. Furthermore, it has been theoretically demonstrated that male and mutual mate choice can evolve in a wide range of circumstances [7–9], especially when females are encountered simultaneously (rather than sequentially), when there is variability in female fertility  or when females prefer males that court intensively .
The hybridizing field crickets - Gryllus firmus and Gryllus pennsylvanicus - form an extensive hybrid zone [13–16] and have multiple barriers to gene exchange [17–21] including an early acting pre-mating behavioral barrier . Conspecific pairs mate faster than heterospecific pairs  and, although male courtship behavior has never been analyzed, the time to mate barrier has been interpreted as female choice. In gryllid crickets females must ultimately mount the male and cooperate in the transfer of the spermatophore, making forced copulation impossible and leaving the ultimate mating decision to the female. Here we test if males are able to regulate courtship intensity depending on whether they encounter a conspecific or heterospecific female.
To understand the speciation process, we need to understand not only the barriers to gene exchange, but also the mechanisms that allow individuals to act differentially towards conspecifics and heterospecifics. Although barriers to gene exchange between G. firmus and G. pennsylvanicus have been well documented, the mechanism behind mate recognition remains elusive (here we avoided the misleading term “species recognition” , using “mate recognition” for within as well as between species mate choice). In most species, recognition mechanisms involve appearance (i.e. morphology) or behavior (e.g. courtship), however the very closely related G. firmus and G. pennsylvanicus are morphologically and behaviorally similar [16, 22]. Pheromones are another common communication mechanism providing reliable long- or short-distance communication. In particular, the non-volatile cuticular hydrocarbons (CHC) play an important role in mate recognition both within and between species in a wide range of insect taxa [24–30], including other crickets [31–33]. CHCs serve as contact pheromones and might be the primary mechanism used by species that lack obvious morphological or behavioral differences . Therefore we also investigate if there are differences in CHC profiles between species that could explain the pre-mating behavioral barrier to gene exchange.
Our results suggest that although females ultimately decide whether or not to mate, males regulate courtship intensity and male choice may play a more important role in the interspecific pre-mating behavioral barrier than previously recognized. Furthermore we report sex specificity in the CHC profiles in both species, and clear differences in CHC composition between female G. firmus and G. pennsylvanicus but not between males of each species. We hypothesize that males may use the CHC profiles as a means to identify conspecifics and adjust their courtship intensity accordingly, leading to differential mating between species.
In August 2010 and 2011, we collected late instar G. firmus (GF) nymphs from Point Judith, RI (41°22′; −71°29′) and Guilford, CT (41°.13′, -72°40′) and G. pennsylvanicus (GP) nymphs in Pownal, VT (42°45′; −73°13′) and Ithaca, NY (42°25′, −76°.29′), all allopatric pure species populations. We sorted nymph crickets by sex and species and raised them at room temperature (25°C) in plastic cages (35 × 31 × 13 cm) with ad libitum food (50/50 mixture of Purina Cat Chow® and LM Bonanza Rabbit Food®), cotton-plugged water vials, and egg cartons for shelter. We separated newly molted adults every 2–3 days.
For the mating trials, we only used females from Pownal, VT (G. pennsylvanicus) and Pt. Judith, RI (G. firmus). Seven- to eight-day old virgin females were haphazardly assigned to one of two treatments. We placed the female in a mating chamber (100 × 25 mm petri dish lined with moist paper) with either a conspecific or heterospecific male. All males had been adults between 7 and 10 days. For each mating pair we recorded the start of courtship behavior, the time to mating, and male and female pronotal width measured to the nearest 0.1 mm. If there was no spermatophore transfer after 60 min, the cross was recorded as failed and the female was immediately placed with another male of the same species. If the second male also failed to mate after 60 min, the cross was recorded as an unsuccessful mating.
Courtship and mating failure data
Proportion of failed courtship (male did not start courtship) and failed mating (no successful mating resulted) data were analyzed using generalized linear models (GLMs) implemented in R. 3.0.1 . The vector of successes and failures was the dependent variable while the independent variables were male species, female species. To avoid using the same female more than once in the analyses, we only considered the 1st male a female was exposed to. We fitted our data to GLM with binomial errors and logit link . Visual inspection of error structure indicated a good fit to the model.
Time to mate data
Only individuals that successfully mated were analyzed for time to mate. Data were analyzed using generalized linear models (GLMs) implemented in R. 3.0.1 . Because this data refers to the time to an event (mating), we fitted it to Gamma errors with inverse link as recommended for survival analysis . Visual inspection of error structure indicated a good fit to the model. The dependent variable was time to mate and independent variables were time to call, male order (1st male the female was exposed to or 2nd male in cases where the 1st male failed to mate), male species and female species and size (females were not duplicated in the analyses, since they were only exposed to a second male if the first failed to mate). We subsequently simplified the model removing variables that were not significant.
For the chemical analysis, we used crickets from all four populations but not the same individuals that were used in the mating trials. Individuals were between 7–15 days old and were kept in same species/sex boxes of 10 individuals each. To avoid plastic contamination and minimize individual to individual contamination, 5–7 days prior to extraction each cricket was individually housed in a glass container.
Because females are larger than males, we extracted cuticular hydrocarbons by placing female crickets in 3 dram glass vials containing 3 mL of HPLC-grade hexane and male crickets in 2 dram glass vials containing 2 mL of HPLC-grade hexane for five to seven minutes . The solution was then filtered with PallLife Sciences Acrodisc (13 mm 0.2 μ nylon membrane) syringe filters to remove particulates, and analyzed with an Agilent Technologies 7890A GC System with an AT 190915–433 30 m × 25 μm × 0.25 μm column attached to an AT 5975C inert XL EI/CI MSD with Triple-Axis Detector MS System, obtaining chromatograms and both EI and CI mass spectra. For the GC method we used a 1 μL or 2 μL injection with an injection temperature of 250°C. The column was held at an initial temperature of 60°C for 4 min followed by a 10°C/min increase to 180°C and then a 3°C/min increase to the final temperature of 260°C, which was then held for 10 min (helium as a carrier gas). All samples were run in duplicate to ensure precision of the GC-MS instrument. Integration parameters were the following: initial area reject = 0; peak width = 0.027; shoulder detection = off; threshold = 14.
To analyze the GC-MS data, we scored a total of 17 peaks representing all seven typical male peaks and most of the female peaks excluding only two peaks that were difficult to score in some individuals (i.e. were a small plateau instead of a peak). To score the peaks, we used the percent of the total area contributed by each peak and then scaled the scored peaks to add up to 100% in each individual. All individuals were scored for all of the 17 peaks; although males rarely exhibited female peaks, females typically exhibited both male and female peaks. These data were analyzed with principal component analysis as performed by the ‘prcomp’ function in “stats” package implemented in R. 3.0.1 . Statistical significance of species, sex and population were assessed by an ANOVA permutation test (10,000 permutations) with the “Vegan” package . After confirming a difference between sexes, we analyzed males and females separately.
For the phonotaxis analysis we also used males from all four populations. Because we were interested in courtship song (used when the female is on sight and the only song our experimental females were exposed to) and not calling song (long distance song to attract females), we placed a male and female cricket of same species in a petri dish lined with moist paper and with holes drilled into the lid. We then placed the petri dish in a chamber (35 × 31 × 13 cm) with a microphone attached to the side. This chamber was then enclosed in a 75 × 50 × 60 cm recording chamber with a constant temperature of 25°C, the outer chamber, originally designed for recording bird songs, was constructed of Lucite and lined on four sides with acoustic foam . The crickets were recorded using an Audio-Technica 8010 condenser microphone, high-pass filtered and amplified (cutoff of 500 Hz), and digitized (16 bit, 44 kHz) using SoundEdit16 (Macromedia). We collected 10-min recordings of the courtship songs of each individual, which were then cut to 10-second clips to highlight areas of high courtship intensity. We measured eight variables from each clip using SoundEdit16: chirp duration, chirp period, pulses per chirp, pulse duration, pulse period, chirp peak frequency, pulse peak frequency, and number of harmonics per chirp. These data were analyzed in the same way as the CHC data (above). Statistical significance of species, population and male age were assessed by an ANOVA permutation test (10,000 permutations).
Courtship and mating failure
Both male species (χ2 = 13.52, df = 5, P < 0.0002) and male–female species interaction (χ2 = 27.87, df = 4, P < 0.0001) were significant in determining the proportion of failed courtship (44 out of 114 trials, Figure 1), female species was not significant. Male G. firmus and males paired to conspecific females were more likely to initiate courtship (GF♂:GF♀: 92.5%, GP♂:GP♀: 77.8%, GF♂:GP♀ 44.4%, GP♂:GF♀: 28.9% courtship success).
Considering only 1st males that initiated courtship (n = 70), the proportion of mating failure (n = 26, Figure 1) was influenced only by the interaction between males and females (χ2 = 10.62, df = 4, P < 0.001) and, as expected, conspecifics were more likely to mate (GF♂:GF♀: 67.6%, GP♂:GP♀: 85.7%, GF♂:GP♀: 12.5%, GP♂:GF♀: 54.5% mating success).
Time to mate
Considering only the 73 (two crosses excluded due to missing data) out of 184 crosses that resulted in successful mating (nGF♂:GF♀: 38; nGP♂:GP♀: 15; nGF♂:GP♀: 5; nGP♂:GF♀: 13), time to mate (Figure 2) was influenced by the time to first call (F1,69 = 6.12, P = 0.01) and male species (F1,67 = 7.63, P = 0.006), G. firmus males mated faster (13.2 min for GF and 24.2 min for GP) and were also more vigorous during courtship (courting louder, continuously, and aggressively walking backward towards the female). Female species and male–female interaction (conspecific or heterospecific) did not influence time to mate (F1,66 = 0.09 and F1,60 = 0.07, P > 0.05 respectively).
Cuticular hydrocarbon analysis
To analyze the gas chromatography results, we scored 17 peaks (Figure 3, Table 1) in 138 individuals (nGP♂ = 26, nGP♀ = 28; nGF♂ = 41, nGF♀ = 43). Of these peaks, seven were present in all males and some of the females (“male peaks”) and ten were present in most females but rarely in males (“female peaks”), if a peak was not present in an individual we recorded the peak abundance as zero. The recorded peaks (Table 1) had the following mean retention time (and standard deviation) in minutes (M for “male peaks” and F for “female peaks”): F1, 34.79 (0.01); F2, 38.14 (0.02); F3, 39.35 (0.01); F4, 39.88 (0.01); F5, 40.52 (0.02); M1, 42.86 (0.02); F6, 43.67 (0.03); F7,44.12 (0.02); M2, 44.43 (0.02); F8, 44.70 (0.03); F9, 45.42 (0.03); M3, 47.39 (0.04); M4, 47.78 (0.04); M5, 48.09 (0.04); M6, 48.55 (0.04); F10, 49.68 (0.03) and M7, 50.87 (0.04) minutes (Figure 3, Table 1).
Principal component analysis of CHC peak abundances (Figure 4 and Additional file 1: Figure S1) showed significant differences between sexes (GP F1,50 = 290.64, P < 0.0001; GF F1,80 = 139.31, P < 0.0001) and species (females F1,67 = 90.51, P = 0.0002; males F1,63 = 24.6, P = 0.0001) but not between populations (males F2,63 = 2.12, P = 0.062; females F2,67 = 1.24, P = 0.25). In contrast, the analysis of CHC composition (peak presence/absence), showed no differences between G. firmus and G. pennsylvanicus males (species F1,63 = 1.06, P = 0.35; populations F2,63 = 1.1, P = 0.35, Figure 3), but significant differences in female CHC composition both between species and populations (species F1,68 = 5.2, P = 0.0001; population F2,68 = 2.5, P = 0.0026).
Males usually exhibited all seven peaks (M1-M7) but occasionally a male would have a very low concentration of female peaks F1, F6, F7, F8 and F9 (Table 1, Figure 3). Females had a more complex and variable CHC profile (Table 1) with up to 19 peaks (two of which were not scored) including all typical male peaks.
In both species, some females had a CHC profile almost identical to the typical male profile (Figure 5 and Additional file 1: Figure S1), with the exception of a higher prevalence of female peaks at very low frequencies in some individuals (typically below 1%). These “male likes” comprised 32.1% (9 out of 28) of the G. pennsylvanicus females and 11.6% (5 out of 43, with only 2 fully inside the male distribution) of the G. firmus females, a nearly statistically significant difference between species (exact Fisher test, P = 0.06).
To roughly categorize compounds present in the cuticular hydrocarbon mixture, we analyzed commercially available alkanes and alcohol standard mixtures. The retention times of the peaks in these mixtures did not match any of the profile peaks, but suggest that any alkanes present in the cricket hydrocarbon profile contain 23–34 carbons, indicating that the compounds present are not particularly volatile.
We quantified courtship song characteristics in 16 G. pennsylvanicus and 11 G. firmus males. From the power spectra analysis, energy of both species’ songs is concentrated in the range between 2 kHz and 16 kHz, with spectral maxima always between 4 and 5 kHz.
The three most variable measurements were chirp period, pulse duration, and pulses per chirp. Although there was a high degree of variation among the individuals (CV ranging from 21% to 58% with a similar distribution between the two species), PC analysis of the eight courtship song variables showed no significant differences between species (F1,20 = 0.75, P = 0.41), populations (F2,20 = 0.19, P = 0.89) or an effect of male age (F1,20 = 0.09, P = 0.86). Pulse rate (the inverse of pulse period), which was suggested to be a driving factor in female preferences in Laupala cerasina and L. eukolea crickets , was more variable in G. firmus than G. pennsylvanicus (F- test, F15,10 = 6.03, P < 0.05) but did not show differences in magnitude between the two species (Wilcoxon Rank Sum test U11,16 = 123, P > 0.05).
The present study reports previously uncharacterized male courtship behavior and cuticular hydrocarbon profiles in the hybridizing crickets Gryllus firmus and G. pennsylvanicus. These two species exhibit multiple barriers to gene exchange that act throughout their life history, including a behavioral barrier that results in increased time to mate in heterospecific pairs (Maroja et al. ). This reluctance to mate with heterospecifics has previously been attributed to a female mate choice. Here, we demonstrated that male mate choice (as courtship effort allocation) plays a more important role in the interspecific prezygotic behavioral barrier than previously recognized. Furthermore cuticular hydrocarbon composition, which differs between females of the two species but not between males, might be the mechanism underlying male mate choice.
Male courtship effort influences mating
Male courtship effort was an important factor in mating outcome (Figures 1 and 2). Our results show that males often fail to court heterospecific females (there was more courtship in conspecific pairs). When courtship intensity is sufficient to elicit a female response (i.e. successful mating), the female species did not influence time to mate (there was no significant effect of female species or male–female interaction on time to mate). This suggests that females will mate to any male that is vigorously courting. Heterospecific pairs are also more likely to fail to mate (Figure 1). Either females favor conspecific males for reasons unrelated to courtship effort or heterospecific males court less vigorously and thus fall below the threshold to elicit a female response .
In conspecific crosses, Gryllus firmus males typically courted vigorously and continuously throughout the mating trial. In contrast, G. pennsylvanicus males courted less vigorously (softly and did not walk aggressively towards the female) and often with an extensive time between first courtship effort and subsequent calling bouts (LSM and JJ personal observation). This difference in courtship intensity and aggression could be a result of differences in population densities; G. firmus often have higher population densities and less spaced populations than G. pennsylvanicus (e.g. ). Increased population densities have been shown to lead to agonistic behavior in field crickets, when populations densities are manipulated  or change naturally over the season . Crickets in this study were exposed to constant densities in the lab (10–15 conspecifics of same sex), but it is possible that G. firmus has evolved agonistic behaviors as result of differences in natural populations. This could lead to more male-male competition (e.g. ) and increased courtship effort by G. firmus males. This difference in behavior could explain why females of both species mated faster with the vigorously courting G. firmus. It is also important to notice that heterospecific courtship effort from a G. pennsylvanicus male represents a clear case of misdirected courtship  as G. firmus females are unable to produce hybrid offspring . Heterospecific courtship is thus more wasteful for a G. pennsylvanicus male than G. firmus male. Our results suggest that the prezygotic behavioral barrier (time to mate), originally interpreted as female choice , may be strongly influenced by male courtship effort. Indeed, results pointing towards faster time to mate in conspecific pairs were observed only in G. firmus females (and male courtship effort was not quantified ), which could suggest asymmetrical female preference (as observed in grasshoppers ) or could be interpreted as a result of the vigorous courtship behavior of G. firmus males in relation to the heterospecific G. pennsylvanicus males. The faster time to mate in conspecific G. firmus pairs can thus be explained by differential courtship effort between species, and not only by a preference for conspecifics by G. firmus females.
Courtship behavior is a costly signal (e.g. [46–48]) and it is thought to act as an honest signal of male quality [49, 50]; males that court less vigorously might represent low quality individuals [3, 40]. As a result it can be difficult to differentiate male choice through adjustments in courtship intensity from female choice based on male courtship vigor. The fact that we observed males differentially courting conspecific and heterospecific females, suggests that males adjust courtship effort to particular female types and that this is not simply a reflection of male quality. It is interesting that the very tendency of females to select males by courtship effort (well documented gryllids [51–54]) might lead to the evolution of male choosiness even in species where males do not invest in offspring. South et al.  modeled a system in which male choosiness evolved when females preferred males that courted with added intensity. Males that invested a larger proportion of their total courtship effort towards preferred females tended to acquire matings, thereby increasing the frequency of male “choosiness” alleles. In this scenario female choice plays a direct role on the evolution of male choice. Indeed, in many species males have been shown to be selective, as demonstrated in the allocation of sperm in Gryllus and allocation of courtship effort in many other animal species [6, 56–59]. Here males were selective by adjusting their courtship effort toward the preferred (conspecific) females, and females were selective by mating with more intensely courting males, which happened to (usually) be conspecifics.
No differences in courtship song
While the barriers to gene exchange in this system have been well documented [17–21], the mechanism underlying mate recognition remains elusive. Gryllus firmus and G. pennsylvanicus are morphologically very similar , and although calling song is slightly different between species and females from allopatric populations exhibit preference to conspecifics , courtship song has not been thought to play a role in mate recognition . In other Gryllus species, it did not diverge as much as the calling song . Indeed many female Gryllus have preferences for particular calling songs and may use them to identify conspecific males [60, 63–66], while courtship song (the only songs females in this study were exposed to) does not seem to be as important. In many gryllids the intensity of courtship behavior (rather than the characteristics of song) is the determining factor in male mating success [67, 68]; even muted males can acquire matings after proper courtship behavior . We confirmed that the differential success between conspecific and heterospecific crosses is unlikely to be the result of differences in courtship song, which did not differ between the two species, despite the very high variability among individuals (see Results). This is consistent with previous studies indicating that many variations in courtship song will elicit mating and that the lack of directional selection on courtship song may lead to increased variation in natural populations due to random drift [67–69].
Cuticular hydrocarbons may mediate mate choice
Because males court conspecific and heterospecific females differentially - G. firmus and G. pennsylvanicus males court heterospecifics at 58.6% and 48.7% of the rate for conspecifics, respectively (and mate at 35.3% and 70.3%, respectively) - they must be able to differentiate between female species. We suggest that the cuticular hydrocarbon profile might be the phenotypic signal used for mate recognition. In crickets, antennal contact and vibration are essential to the initiation of courtship [41, 70], and also ensure detection of the CHC non-volatile compounds. The importance of CHC for male courtship behavior has been demonstrated in Gryllus bimaculatus[32, 71] where males only exhibited courtship behavior towards females with CHC extracts, and Teleogryllus oceanicus which use CHC to identify genetic similarity [33, 72] and increase female motivation to reach a calling male . To test the role of CHC compounds in G. firmus and G. pennsylvanicus mate recognition we conducted preliminary experiments exposing G. firmus males to female CHC extracts. Extract on filter disks led to agitated behavior, but no courtship. A few males responded to female cadavers with CHCs intact (2 out of 4) and female cadavers stripped of CHC and re-painted with CHC extract (2 out of 5), but no males courted female cadavers stripped of CHC (n = 5). Together with the CHC differences observed between female species, these preliminary results suggest that CHC might be the mechanism behind the pre-mating behavioral gene exchange barrier.
We observed large differences between male and female CHC profiles within each species (Figure 4); males never exhibited five of the 10 female peaks and most females exhibited more peaks than any male (Figure 3). This sexual dimorphism has also been observed in G. bimaculatus and other orthoptera species [33, 71] and it is assumed to be a result of sexual selection . Surprisingly, G. firmus and G. pennsylvanicus males had the same CHC composition, although with differences in peak abundances, while females had differences in both composition and abundance of CHC peaks (albeit with overlap, Figures 3 and 4). It is possible, that males use the CHC profiles to discriminate between conspecific and heterospecific females and adjust their courtship intensity accordingly.
Interestingly some females had CHC composition similar to that of a male (“male-like”, Figures 4 and 5). These females constituted 32.1% of the G. pennsylvanicus and 11.6% of the G. firmus. To our knowledge, CHC “male like” females (Figure 5) have not yet been described and we currently do not know the behavioral implications of this phenotype. Perhaps it represents an example of male mimicry driven by sexual conflict ; more specifically a chemical, rather than morphological or behavioral, male mimicry. Indeed male mimicry (morphological and behavioral) is common in many species of odonata, and has evolved as a sexual harassment avoidance strategy [76–78]. Sexual harassment by males disturbs female energy budgets and may cause physical harm, ultimately reducing fecundity [76, 79, 80]. In crickets, male harassment is known to reduce female longevity , males will also attempt to sequester females into burrows [82, 83], mate guard [84, 85] and may aggressively prevent spermatophore detachment . It is possible that the excessive polymorphism observed in female CHC profiles may confuse males  and reduce excessive male attention by interfering with the mechanisms males use to recognize potential mates, making them unable to effectively adapt to any particular female morph  and reducing harassment to the unusual female phenotypes. Furthermore, because this “male-like” phenotype is common to both species, it is possible that most heterospecific crosses happen through these females, we are currently investigating this hypothesis.
Here we demonstrated that male choice plays a larger role in the pre-mating interspecific behavioral barrier than previously recognized and that the CHC composition might be behind the mate recognition mechanism. Cuticular hydrocarbon profiles are not only different between intraspecific males and females, but differ between females of the two species but not between males. Further studies need to be conducted to understand the implications of the female CHC “male like” phenotype and to confirm that males are indeed capable of differentiating between different female CHC profiles.
Coyne JA, Orr HA: Speciation. 2004, Sunderland, Mass: Sinauer Associates, Inc. Publishers
Andersson MB: Sexual Selection. 1994, Princeton, N.J.: Princeton University Press
Edward DA, Chapman T: The evolution and significance of male mate choice. Trends Ecol Evol. 2011, 26 (12): 647-654. 10.1016/j.tree.2011.07.012.
Amundsen T, Forsgren E: Male mate choice selects for female coloration in a fish. Proc Natl Acad Sci U S A. 2001, 98 (23): 13155-13160. 10.1073/pnas.211439298.
Bel-Venner MC, Dray S, Allaine D, Menu F, Venner S: Unexpected male choosiness for mates in a spider. Proc R Soc B-Biol Sci. 2008, 275 (1630): 77-82. 10.1098/rspb.2007.1278.
Reading KL, Backwell PRY: Can beggars be choosers? Male mate choice in a fiddler crab. Anim Behav. 2007, 74: 867-872. 10.1016/j.anbehav.2006.09.025.
Kokko H, Monaghan P: Predicting the direction of sexual selection. Ecol Lett. 2001, 4 (2): 159-165. 10.1046/j.1461-0248.2001.00212.x.
Kokko H, Johnstone RA: Why is mutual mate choice not the norm? Operational sex ratios, sex roles and the evolution of sexually dimorphic and monomorphic signalling. Philos Trans R Soc Lond B Biol Sci. 2002, 357 (1419): 319-330. 10.1098/rstb.2001.0926.
Servedio MR, Lande R: Population genetic models of male and mutual mate choice. Evolution. 2006, 60 (4): 674-685.
South SH, Arnqvist G, Servedio MR: Female preference for male courtship effort can drive the evolution of male mate choice. Evolution. 2012, 66 (12): 3722-3735. 10.1111/j.1558-5646.2012.01716.x.
Scudder SH: The species of Gryllus found in the United States east of Sierra Nevadas. Psyche. 1902, 9 (309): 294-295.
Harrison RG, Arnold J: A narrow hybrid zone between closely related cricket species. Evolution. 1982, 36 (3): 535-552. 10.2307/2408099.
Harrison RG, Bogdanowicz SM: Patterns of variation and linkage disequilibrium in a field cricket hybrid zone. Evolution. 1997, 51 (2): 493-505. 10.2307/2411122.
Maroja LS, Andres JA, Harrison RG: Genealogical discordance and patterns of introgression and selection across a cricket hybrid zone. Evolution. 2009, 63 (11): 2999-3015. 10.1111/j.1558-5646.2009.00767.x.
Larson EL, Becker CG, Bondra ER, Harrison RG: Structure of a mosaic hybrid zone between the field crickets Gryllus firmus and G. pennsylvanicus. Ecology and Evolution. 2013, 3 (4): 985-1002. 10.1002/ece3.514.
Harrison RG: Barriers to gene exchange between closely related cricket species.1. Laboratory hybridization studies. Evolution. 1983, 37 (2): 245-251. 10.2307/2408333.
Harrison RG: Barriers to gene exchange between closely related cricket species.2. Life-cycle variation and temporal isolation. Evolution. 1985, 39 (2): 244-259. 10.2307/2408360.
Ross CL, Harrison RG: A fine-scale spatial analysis of the mosaic hybrid zone between Gryllus firmus and Gryllus pennsylvanicus. Evolution. 2002, 56 (11): 2296-2312.
Ross CL, Harrison RG: Viability selection on overwintering eggs in a field cricket mosaic hybrid zone. Oikos. 2006, 115 (1): 53-68. 10.1111/j.2006.0030-1299.15054.x.
Larson EL, Hume GL, Andres JA, Harrison RG: Post-mating prezygotic barriers to gene exchange between hybridizing field crickets. J Evol Biol. 2012, 25 (1): 174-186. 10.1111/j.1420-9101.2011.02415.x.
Maroja LS, Andres JA, Walters JR, Harrison RG: Multiple barriers to gene exchange in a field cricket hybrid zone. Biol J Linn Soc. 2009, 97 (2): 390-402. 10.1111/j.1095-8312.2009.01201.x.
Mendelson TC, Shaw KL: The (mis)concept of species recognition. Trends Ecol Evol. 2012, 27 (8): 421-427. 10.1016/j.tree.2012.04.001.
Etges WJ, Ahrens MA: Premating isolation is determined by larval-rearing substrates in cactophilic Drosophila mojavensis. V. Deep geographic variation in epicuticular hydrocarbons among isolated populations. Am Nat. 2001, 158 (6): 585-598. 10.1086/323587.
Etges WJ, de Oliveira CC, Ritchie MG, Noor MAF: Genetics of incipient speciation in drosophila mojavensis: Ii. Host plants and mating status influence cuticular hydrocarbon Qtl expression and G X E interactions. Evolution. 2009, 63 (7): 1712-1730. 10.1111/j.1558-5646.2009.00661.x.
Howard RW, Mcdaniel CA, Nelson DR, Blomquist GJ, Gelbaum LT, Zalkow LH: Cuticular hydrocarbons of reticulitermes virginicus (banks) (isoptera, rhinotermitidae) and their role as potential species-recognition and caste-recognition cues. J Chem Ecol. 1982, 8 (9): 1227-1239. 10.1007/BF00990755.
Howard RW, Blomquist GJ: Ecological, behavioral, andbiochemical aspects of insect hydrocarbons. Annu Rev Entomol. 2005, 50: 371-393. 10.1146/annurev.ento.50.071803.130359.
Peterson MA, Dobler S, Larson EL, Juarez D, Schlarbaum T, Monsen KJ, Francke W: Profiles of cuticular hydrocarbons mediate male mate choice and sexual isolation between hybridising Chrysochus (Coleoptera: Chrysomelidae). Chemoecology. 2007, 17 (2): 87-96. 10.1007/s00049-007-0366-z.
Buellesbach J, Gadau J, Beukeboom LW, Echinger F, Raychoudhury R, Werren JH, Schmitt T: Cuticular hydrocarbon divergence in the jewel wasp Nasonia: evolutionary shifts in chemical communication channels?. J Evol Biol. 2013, 26 (11): 2467-2478. 10.1111/jeb.12242.
Havens JA, Etges WJ: Premating isolation is determined by larval rearing substrates in cactophilic Drosophila mojavensis. IX. Host plant and population specific epicuticular hydrocarbon expression influences mate choice and sexual selection. J Evol Biol. 2013, 26 (3): 562-576. 10.1111/jeb.12073.
Mullen SP, Mendelson TC, Schal C, Shaw KL: Rapid evolution of cuticular hydrocarbons in a species radiation of acoustically diverse Hawaiian crickets (Gryllidae: Trigonidiinae: Laupala). Evolution. 2007, 61 (1): 223-231. 10.1111/j.1558-5646.2007.00019.x.
Nagamoto J, Aonuma H, Hisada M: Discrimination of conspecific individuals via cuticular pheromones by males of the cricket Gryllus bimaculatus. Zool Sci. 2005, 22 (10): 1079-1088. 10.2108/zsj.22.1079.
Thomas ML, Simmons LW: Sexual dimorphism in cuticular hydrocarbons of the Australian field cricket Teleogryllus oceanicus (Orthoptera: Gryllidae). J Insect Physiol. 2008, 54 (6): 1081-1089. 10.1016/j.jinsphys.2008.04.012.
R Core Team: R: A Language and Environment for Statistical Computing. 2013, Vienna, Austria: R Foundation for Statistical Computing, http://www.R-project.org/,
Crawley MJ: The R book. 2007, Chichester, England: Hoboken, N.J.: Wiley
Ming QL, Lewis SM: Mate recognition and sex differences in cuticular hydrocarbons of the diurnal firefly Ellychnia corrusca (Coleoptera: Lampyridae). Ann Entomol Soc Am. 2010, 103 (1): 128-133. 10.1603/008.103.0116.
Oksanen J, Blanchet FG, Kindt R, Legendre P, Solymos M, Stevens HH, Wagner H, Minchin PROHRB, G.L. S, P: Vegan: community ecology package. 2013, R package version 20–8, http://CRAN.R-project.org/package=vegan
Williams H, Mehta N: Changes in adult zebra pinch song require a forebrain nucleus that is not necessary for song production. J Neurobiol. 1999, 39 (1): 14-28. 10.1002/(SICI)1097-4695(199904)39:1<14::AID-NEU2>3.0.CO;2-X.
Oh KP, Fergus DJ, Grace JL, Shaw KL: Interspecific genetics of speciation phenotypes: song and preference coevolution in Hawaiian crickets. J Evol Biol. 2012, 25 (8): 1500-1512. 10.1111/j.1420-9101.2012.02531.x.
Byers J, Hebets E, Podos J: Female mate choice based upon male motor performance. Anim Behav. 2010, 79 (4): 771-778. 10.1016/j.anbehav.2010.01.009.
Adamo SA, Hoy RR: Mating behaviour of the field cricket Gryllus bimaculatus and its dependence on social and environmental cues. Anim Behav. 1994, 47 (4): 857-868. 10.1006/anbe.1994.1117.
Hissmann K: Strategies of mate finding in the European field cricket (Gryllus campestris) at different population densities: a field study. Ecological Entomology. 1990, 15 (3): 281-291. 10.1111/j.1365-2311.1990.tb00810.x.
Saleh N, Larson E, Harrison R: Reproductive success and body size in the cricket Gryllus firmus. J Insect Behav. 2013, 1-11. doi:10.1007/s10905-013-9425-1
Groning J, Hochkirch A: Reproductive interference between animal species. Q Rev Biol. 2008, 83 (3): 257-282. 10.1086/590510.
Hochkirch A, Lemke I: Asymmetric mate choice, hybridization, and hybrid fitness in two sympatric grasshopper species. Behav Ecol Sociobiol. 2011, 65 (8): 1637-1645. 10.1007/s00265-011-1174-6.
Clutton-Brock T, Langley P: Persistent courtship reduces male and female longevity in captive tsetse flies Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Behav Ecol. 1997, 8 (4): 392-395. 10.1093/beheco/8.4.392.
Cordts R, Partridge L: Courtship reduces longevity of male Drosophila melanogaster. Anim Behav. 1996, 52 (2): 269-278. 10.1006/anbe.1996.0172.
Mappes J, Alatalo RV, Kotiaho J, Parri S: Viability costs of condition-dependent sexual male display in a drumming wolf spider. Proc R Soc Lond Ser B Biol Sci. 1996, 263 (1371): 785-789. 10.1098/rspb.1996.0117.
Pomiankowski A: Sexual selection - the handicap principle does work sometimes. Proc R Soc Ser B-Bio. 1987, 231 (1262): 123-145. 10.1098/rspb.1987.0038.
Johnstone RA: Sexual selection, honest advertisement and the handicap principle - reviewing the evidence. Biol Rev Camb Philos Soc. 1995, 70 (1): 1-65. 10.1111/j.1469-185X.1995.tb01439.x.
Burk T: Male Aggression and Female Choice in a Field Cricket (Teleogryllus Oceanicus): the Importance of Courtship Song. Orthopteran Mating Systems - Sexual Competition in a Diverse Group of Insects. Edited by: Gwynne DT, Morris GK. 1983, : Boulder: Westview Press, 97-119.
Libersat F, Murray JA, Hoy RR: Frequency as a releaser in the courtship song of two crickets, Gryllus bimaculatus (de Geer) and Teleogryllus oceanicus: a neuroethological analysis. J Comp Physiol A. 1994, 174 (4): 485-494.
Nelson CM, Nolen TG: Courtship song, male agonistic encounters, and female mate choice in the house cricket, Acheta domesticus (Orthoptera: Gryllidae). J Insect Behav. 1997, 10 (4): 557-570. 10.1007/BF02765377.
Rebar D, Bailey NW, Zuk M: Courtship song’s role during female mate choice in the field cricket Teleogryllus oceanicus. Behav Ecol. 2009, 20 (6): 1307-1314. 10.1093/beheco/arp143.
Thomas ML, Simmons LW: Male crickets adjust the viability of their sperm in response to female mating status. Am Nat. 2007, 170 (2): 190-195. 10.1086/519404.
Ruiz M, Beals ZM, Martins EP: Male sagebrush lizards (sceloporus graciosus) increase exploratory behavior toward females with more courtship experience. Herpetologica. 2010, 66 (2): 142-147. 10.1655/09-022R2.1.
Roberts JA, Uetz GW: Information content of female chemical signals in the wolf spider, Schizocosa ocreata: male discrimination of reproductive state and receptivity. Anim Behav. 2005, 70: 217-223. 10.1016/j.anbehav.2004.09.026.
Lihoreau M, Zimmer C, Rivault C: Mutual mate choice: when it pays both sexes to avoid inbreeding. Plos One. 2008, 3 (10): e3365-10.1371/journal.pone.0003365. 3361–3367
Herdman EJE, Kelly CD, Godin J-GJ: Male mate choice in the guppy (Poecilia reticulata): do males prefer larger females as mates?. Ethology. 2004, 110 (2): 97-111. 10.1111/j.1439-0310.2003.00960.x.
Doherty JA, Storz MM: Calling song and selective phonotaxis in the field crickets, Gryllus firmus and G. pennsylvanicus (Orthoptera: Gryllidae). J Insect Behav. 1992, 5 (5): 555-569. 10.1007/BF01048004.
Alexander RD: Life cycle origins, speciation, and related phenomena in crickets. Q Rev Biol. 1968, 43: 1-41. 10.1111/j.1469-185X.1968.tb01106.x.
Fitzpatrick MJ, Gray DA: Divergence between the courtship songs of the field crickets Gryllus texensis and Gryllus rubens (Orthoptera, Gryllidae). Ethology. 2001, 107 (12): 1075-1085. 10.1046/j.1439-0310.2001.00730.x.
Grace JL, Shaw KL: Coevolution of male mating signal and female preference during early lineage divergence of the hawaiian cricket, Laupala Cerasina. Evolution. 2011, 65 (8): 2184-2196. 10.1111/j.1558-5646.2011.01278.x.
Zuk M, Simmons LW: Reproductive Strategies of the Crickets (Orthoptera: Gryllidae). The Evolution of Mating Systems in Insects and Arachnids. Edited by: Choe JC, Crespi BJ. 1997, Cambridge, New York & Melbourne: Cambridge University Press, 89-109.
Gray DA, Cade WH: Sexual selection and speciation in field crickets. Proc Natl Acad Sci U S A. 2000, 97 (26): 14449-14454. 10.1073/pnas.97.26.14449.
Honda-Sumi E: Difference in calling song of three field crickets of the genus Teleogryllus: the role in premating isolation. Anim Behav. 2005, 69 (4): 881-889. 10.1016/j.anbehav.2004.05.015.
Balakrishnan R, Pollack GS: Recognition of courtship song in the field cricket, Teleogryllus oceanicus. Anim Behav. 1996, 51 (2): 353-366. 10.1006/anbe.1996.0034.
Zuk M, Rebar D, Scott SP: Courtship song is more variable than calling song in the field cricket Teleogryllus oceanicus. Anim Behav. 2008, 76: 1065-1071. 10.1016/j.anbehav.2008.02.018.
Bailey NW, McNabb JR, Zuk M: Preexisting behavior facilitated the loss of a sexual signal in the field cricket Teleogryllus oceanicus. Behav Ecol. 2008, 19 (1): 202-207.
Dambach M: Vibrational Responses. Cricket Behavior and Neurobiology. Edited by: Huber F, Moore TE, Loher W. 1989, Ithaca: Cornell University Press, 178-197.
Tregenza T, Wedell N: Definitive evidence for cuticular pheromones in a cricket. Anim Behav. 1997, 54: 979-984. 10.1006/anbe.1997.0500.
Thomas ML, Simmons LW: Crickets detect the genetic similarity of mating partners via cuticular hydrocarbons. J Evol Biol. 2011, 24 (8): 1793-1800. 10.1111/j.1420-9101.2011.02319.x.
Bailey NW: Mate choice plasticity in the field cricket Teleogryllus oceanicus: effects of social experience in multiple modalities. Behav Ecol Sociobiol. 2011, 65 (12): 2269-2278. 10.1007/s00265-011-1237-8.
Chenoweth SF, Blows MW: Contrasting mutual sexual selection on homologous signal traits in Drosophila serrata. Am Nat. 2005, 165 (2): 281-289. 10.1086/427271.
Arnqvist G, Rowe L: Sexual Conflict. 2005, Princeton, N.J.: Princeton University Press
Gosden TP, Svensson EI: Density-dependent male mating harassment, female resistance, and male mimicry. Am Nat. 2009, 173 (6): 709-721. 10.1086/598491.
Svensson EI, Abbott JK, Gosden TP, Coreau A: Female polymorphisms, sexual conflict and limits to speciation processes in animals. Evol Ecol. 2009, 23 (1): 93-108. 10.1007/s10682-007-9208-2.
Cordoba-Aguilar A: Dragonflies and Damselflies: Model Organisms for Ecological and Evolutionary Research. 2008, Oxford: New York: Oxford University Press
Bots J, De Bruyn L, Van Dongen S, Smolders R, Van Gossum H: Female polymorphism, condition differences, and variation in male harassment and ambient temperature. Biol J Linn Soc. 2009, 97 (3): 545-554. 10.1111/j.1095-8312.2009.01238.x.
Gay L, Eady PE, Vasudev R, Hosken DJ, Tregenza T: Costly sexual harassment in a beetle. Physiol Entomol. 2009, 34 (1): 86-92. 10.1111/j.1365-3032.2008.00656.x.
Bateman PW, Ferguson JWH, Yetman CA: Courtship and copulation, but not ejaculates, reduce the longevity of female field crickets (Gryllus bimaculatus). J Zool. 2006, 268 (4): 341-346. 10.1111/j.1469-7998.2006.00054.x.
Simmons LW: Sexual selection and body size in a natural population of field cricket, Gryllus campestris (L.). J Orthoptera Res. 1992, 1: 12-13.
Bateman PW: Burrow residency, access to females and body size in male Scapsipedus meridianus Otte & Cade (Orthoptera: Gryllidae; Gryllinae). J Orthoptera Res. 2000, 9: 27-29.
Sakaluk SK: Post-copulatory mate guarding in decorated crickets. Anim Behav. 1991, 41 (2): 207-216. 10.1016/S0003-3472(05)80472-5.
Bateman PW, MacFadyen DN: Mate guarding in the cricket Gryllodes sigillatus: influence of multiple potential partners. Ethology. 1999, 105 (11): 949-957. 10.1046/j.1439-0310.1999.00484.x.
Bussiere LF, Hunt J, Jennions MD, Brooks R: Sexual conflict and cryptic female choice in the black field cricket, Teleogryllus commodus. Evolution. 2006, 60 (4): 792-800.
Fincke OM: Polymorphic signals of harassed female odonates and the males that learn them support a novel frequency-dependent model. Anim Behav. 2004, 67 (5): 833-845. 10.1016/j.anbehav.2003.04.017.
Gavrilets S, Waxman D: Sympatric speciation by sexual conflict. Proc Natl Acad Sci U S A. 2002, 99 (16): 10533-10538. 10.1073/pnas.152011499.
This study was supported by a grant from the Orthopterist society to L.S.M. We thank Heather Williams for lending us her sound equipment and Richard G. Harrison and his lab for great comments and suggestions.
The authors declare that they have no competing interests.
LSM conceived and designed the experiments, analyzed the data and wrote the manuscript. DPR designed the CHC experiments and conducted the chemical analysis. The undergraduate students ZM, JJ and EH performed the experiments, participated in data analysis and wrote the first draft of parts of the manuscript. ELL participated in the experimental design, data collection and manuscript writing. All authors read, made comments on and approved the final manuscript.
Electronic supplementary material
Additional file 1: Figure S1: Principal component analysis of CHC abundances. a) Gryllus firmus and b) G. pennsylvanicus c) males of both species and d) females of both species, “male-like” females are highlighted in a grey background (these females fall inside the male cloud when shown with males). Colors represent populations: G firmus, the beach cricket, is shown in sand colors yellow (Guilford, CT) and orange (Pt Judith, RI) while the inland field cricket, G. pennsylvanicus, in is shown in dark green (Ithaca, NY) and light green (Pownal, VT). In a) and b) the “male-like” females are within the male cloud and may not be easy to distinguish. In c) and d) individuals marked with an “*” where used in Figure 3 and in d) the “male-like” female marked with a “+” was used in Figure 5. (TIFF 2 MB)
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Maroja, L.S., McKenzie, Z.M., Hart, E. et al. Barriers to gene exchange in hybridizing field crickets: the role of male courtship effort and cuticular hydrocarbons. BMC Evol Biol 14, 65 (2014). https://doi.org/10.1186/1471-2148-14-65
- Gryllus firmus
- Gryllus pennsylvanicus
- Barrier to gene exchange