Fig. 5

Analysis of rotenone uptake in N2 and him-5 strains. (A) Dose response curves for rotenone exposure in N2 and him-5 strains expressed in terms of the raw value of the worm volume following a 72-hour exposure to rotenone. Significance was assessed by a two-way ANOVA for strain and dose, strain: p = 0.1342, dose: ***, p < 0.0001, interaction: p = 0.6392. (B) Dose response curves for rotenone exposure in N2 and him-5 strains with worm volume expressed as a percentage of the control. Significance was assessed by a two-way ANOVA for strain and dose, strain: p = 0.4153, dose: ***, p < 0.0001, interaction: p = 0.6118. For graphs in panels A and B, the x-axis represents the log transform of the rotenone concentration, where the doses were 0, 0.125, 0.25, and 0.5 µM. For the 0 µM dose, a value of 0.01 was used to log transform the data, so -2 represents the vehicle control. (C) Detected concentrations of rotenone in the dosing media following the 72-hour exposure. On the x-axis is the intended dose and on the y-axis is the detected amount remaining at the time of harvesting worms for analysis. Significance was assessed by a two-way ANOVA assessing strain and dose, strain: p = 0.7838, dose: ***, p < 0.0001, interaction: p = 0.2697. (D) Detected internal concentrations of rotenone in the worms following the 72-hour exposure. On the x-axis is the intended dose and on the y-axis is the internal dose calculated using the worm volume determined using Wormsizer. Significance was assessed by a two-way ANOVA assessing strain and dose with a Šidák post hoc test for multiple comparisons, strain: p = 0.0513, dose: ***, p < 0.0001, interaction: *, p = 0.0501. Three biological replicates were assessed