Fig. 4

Wild type hermaphrodites and males have similar sensitivity to developmental rotenone exposure. (A) Dose response curves for rotenone exposure in males and hermaphrodites expressed in terms of the raw value of the worm volume following a 72-hour exposure to rotenone. Significance was assessed by a two-way ANOVA for sex and dose, sex: ***, p < 0.0001, dose: ***, p < 0.0001, interaction: *, p = 0.0196. (B) Dose response curves for rotenone exposure in males and hermaphrodites with worm volume expressed as a percentage of the control. Significance was assessed by a two-way ANOVA for sex and dose, sex: p = 0.0685, dose: ***, p < 0.0001, interaction: p = 0.5749. For both graphs, the x-axis represents the log transform of the rotenone concentration, where the doses were 0, 0.125, 0.25, and 0.5 µM. For the 0 µM dose, a value of 0.01 was used to log transform the data, so -2 represents the vehicle control. Three biological replicates were assessed, each with > 50 worms assessed per replicate