Fig. 3
From: Lack of detectable sex differences in the mitochondrial function of Caenorhabditis elegans
Seahorse respirometry analysis across male enriched strains. (A) Representative image of the mitochondrial function parameters assessed using the inhibitors dicyclohexylcarbodiimide (DCCD), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and sodium azide. The Seahorse XFe24 Analyzer determines the oxygen consumption rate (OCR) in pmol O2 per minute prior to and following injections of the compounds. (B) Mean basal OCR across strains. (C) Mean mitochondrial OCR across strains. (D) Mean maximal OCR across strains. (E) Mean spare capacity across strains. (F) Mean proton leak across strains. (G) Mean non-mitochondrial OCR across strains (N2 to him-5p = 0.0419, N2 to him-8p = 0.0481). For figures B-G, the x-axis displays the strain and the y-axis displays the OCR associated with each parameter in pmol/min, normalized to the volume of worm in each well in picoliters. Statistical significance of all pairwise comparisons were assessed using a one-way ANOVA with a Tukey post hoc test for multiple comparisons when normally distributed and a Kruskal-Wallis test with a Dunn’s post-hoc test for multiple comparisons when not normally distributed, as was the case for proton leak. Data represents five biological replicates per strain