Fig. 6

Intraspecific genomic hybridization with emu male and female gDNA probes hybridized in female metaphase chromosomes (a-d). (a) DAPI-stained metaphases of the emu female, (b) hybridization pattern of the male-derived probe (green), (c) hybridization pattern of the female-derived probe (red), and (d) merged images of both genomic probes and DAPI staining. Interspecific genomic hybridization between the emu and the greater rhea (e-h). (e) DAPI-stained metaphases of the emu female, (f) hybridization pattern of the emu female-derived probe (green), (g) hybridization pattern of the greater rhea female-derived probe (red), and (h) merged images of both genomic probes and DAPI staining. While the W chromosome was appropriately identified by a sequential hybridization with the microsatellite (GA)n, the Z chromosome could not be properly identified based on its morphology. Bar = 5 μm