Fig. 8
From: Emerging roles for diguanylate cyclase during the evolution of soma in dictyostelia
Phenotype of thePvio amtCˉ, phyAˉ, dgcAˉamtCˉ and dgcAˉphyAˉmutants. (A) Fruiting bodies. AmtCˉ, phyAˉ, dgcAˉamtCˉ and dgcAˉphyAˉ cells were incubated overnight at 4 °C on KK2 agar and then transferred to 22oC for the indicated time periods until mature fruiting bodies had formed. Scale bars: 0.5 mm. (B) Stalk cells. The fruiting bodies were stained with 0.001% Calcofluor and stalks were photographed under phase contrast (top) and epifluorescence (bottom). Scale bars: 10 μm. Representative images from at least two individual experiments