Fig. 2

The effect of amphibian chemical cues and waterborne TTX on crayfish movement. (a) The number of instances a crayfish moved was significantly reduced in the presence of newt chemical cues (GLMM: p < 0.01) and TTX (GLMM: p < 0.01) treatments relative to the control. The same response was not observed when crayfish were exposed to tree frog chemical cues (p = 0.95). Asterisks show statistically significant treatments relative to the control. (b) Average movement of crayfish during 30-minute trials at four discrete time points shows crayfish continued to move throughout the experiment, but overall movement patterns differ as early as three minutes into the experiment. By the end of the experiment the cumulative number of moves a crayfish made differed between the newt (p < 0.01) and TTX (p < 0.01) treatments relative to the control, but no other pairwise comparisons were significant. Error bars represent the s.e.m