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Table 2 Primers used for PCR amplification and sequencing of the markers COI, 16S, and 28S. Tann = annealing temperature

From: Morphospecies and molecular diversity of ‘lace corals’: the genus Reteporella (Bryozoa: Cheilostomatida) in the central North Atlantic Azores Archipelago

 

Name

Sequence

Tann

Reference

COI

cox1F_prifi

TTGRTTYTTTGGWCAYCCHGAAG

53ºC

[60]

 
 

cox1R_prifi

TCHGARTAHCGNCGNGGTATHCC

53ºC

[60]

 
 

coiR_ret

GCTAGHCCTAGGAARTGTTGA

53ºC

This study

 

16S

16Sar-L

CGCCTGTTTATCAAAAACAT

52–58ºC *

[94]

 
 

16Sbr-H

CCGGTCTGAACTCAGATCACGT

52–58ºC *

[94]

 

28S

28SC1

ACCCGCTGAATTTAAGCAT

55ºC

[95]

 
 

28SC2

TGAACTCTCTCTTCAAAGTTCTTTTC

55ºC

[96]

 
  1. * Tann varied in this range for different species.