Fig. 5

Social behaviors in a resident/intruder assay across multiple cave populations. (A) Representative resident/intruder activity plots for Pachón (top left), Tinaja (bottom left), Los Sabinos (top right) and Molino (bottom right) cavefish during the resident/intruder assay. The number of total behaviors for both the resident and the intruder were combined. All fish were sex and size matched, and sex was not used as a variable given the lack of effect of sex on seven behaviors in Pachón cavefish (Fig S1). (B-H) Quantifications of behaviors annotated during fights with comparisons across populations (Pachón = Pa, Tinaja = Ti, Los Sabinos = Sa, and Molino = Mo). One-way ANOVA followed by Tukey’s multiple comparisons test was performed for circling (Pachón-Molino, p < 0.0001, Pachón-Tinaja, p < 0.05, Pachón-Los Sabinos, p < 0.001, Molino-Tinaja, p = 0.2887, Molino-Los Sabinos, p = 0.885, Tinaja-Los Sabinos, p = 0.7954). Kruskal-Wallis with Dunn’s multiple comparisons test were performed for biting (Pachón-Molino, p = 0.9213, Pachón-Tinaja, p > 0.9999, Pachón-Los Sabinos, p = 0.7564, Molino-Tinaja, p > 0.9999, Molino-Los Sabinos, p > 0.9999, Tinaja-Los Sabinos, p > 0.9999), striking (Pachón-Molino, p < 0.001, Pachón-Tinaja, p > 0.9999, Pachón-Los Sabinos, p = 0.2443, Molino-Tinaja, p = 0.0057, Molino-Los Sabinos, p = 0.3528, Tinaja-Los Sabinos, p > 0.9999), escaping (Pachón-Molino, p < 0.01, Pachón-Tinaja, p = 0.409, Pachón-Los Sabinos, p = 0.4466, Molino-Tinaja, p = 0.206, Molino-Los Sabinos, p = 0.341, Tinaja-Los Sabinos, p > 0.9999), following (Pachón-Molino, p = 0.0585, Pachón-Tinaja, p > 0.9999, Pachón-Los Sabinos, p > 0.9999, Molino-Tinaja, p = 0.2307, Molino-Los Sabinos, p = 0.3641, Tinaja-Los Sabinos, p > 0.9999), freezing (Pachón-Molino, p = 0.1938, Pachón-Tinaja, p > 0.9999, Pachón-Los Sabinos, p > 0.9999, Molino-Tinaja, p = 0.0586, Molino-Los Sabinos, p = 0.0995, Tinaja-Los Sabinos, p > 0.9999), and avoidance (Pachón-Molino, p = 0.0706, Pachón-Tinaja, p > 0.9999, Pachón-Los Sabinos, p > 0.9999, Molino-Tinaja, p = 0.0702, Molino-Los Sabinos, p = 0.0289, Tinaja-Los Sabinos, p > 0.9999). Significance: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), not significant (ns)