Fig. 5

A. Phylogenetic analysis of candidate ABP MYB regulators identified in Ruellia petal transcripts plus orthologs that have been functionally validated to regulate the ABP in other flowering plant species (Arabidopsis thaliana, Petunia x hybrida, and Pyrus pyrifolia, highlighted in green; four MYB orthologs from Mimulus guttatus were also included in phylogenetic analyses given the close evolutionary relationship of Mimulus to Ruellia, but these four copies have not been functionally validated in prior works). Four, strongly supported clades of candidate MYBs in Ruellia were recovered (highlighted in yellow). B. Protein sequence alignment of candidate ABP MYB regulators in Ruellia and orthologs in other species. Relative expression of each gene shown in FPKM (fragments per kilobase of transcript per million fragments sequenced). Results indicate that copies from the MYB10L1 clade in Ruellia were among the most highly expressed and also contain an amino acid residue ('ANDV') that has been reported to characterize MYBs that regulate the ABP (see text); this residue was also found copies from the MYB10L4 clade, but transcripts in this clade were in low abundance. In contrast, a non-synonymous substitution yielding an 'ANDI' residue characterized most of the candidate Ruellia MYBs in the MYB10L2 and MYB10L3 clades, and this residue is conserved across MYBs that function elsewhere besides in ABP production in other plant families