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Fig. 3 | BMC Ecology and Evolution

Fig. 3

From: Co-expression network analyses of anthocyanin biosynthesis genes in Ruellia (Wild Petunias; Acanthaceae)

Fig. 3

Relative expression of F3'H and DFR copies in Ruellia simplex during flower development based on qRT-PCR. Relative expression levels were calculated based on transcript abundance of the 18S gene in Ruellia using formula: Ct(18s)-Ct(gene). A. Five stages of floral development, from bud to fully anthetic flower. B. Stage-specific expression of DFR. C. Stage-specific expression of F3'H (note that F3'H1 was not expressed in R. simplex). Results show that DFR3 as well as F3'H2 and F3'H3 peak in expression levels during stage 2, suggesting possible coordinated regulation of these three loci

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