Fig. 9

In situ hybridization results for Wnt genes in stage 14 H. exemplaris embryos. Green color represents gene expression. Nuclei are labeled with DAPI (blue). Images that share the same letter represent data from the same embryo. Arrows point to the developing pharynx. Asterisks mark the region between the posteriormost legs. Anterior is towards the top. a, b Wnt5 expression. a Ventral surface of legs is in view. b, b’ Dorsal surface of legs is toward the outside and the ventral surface is toward the inside of the embryo. c, d Wnt11 expression. d Bilateral view. The posterior end of embryo is outlined. e, f Wnt16B expression. g WntA expression. a–b’’, e, f’, f’’ Dashed lines outline legs. a, c, e, g Lateral view of embryos that are facing right. b–b’’, f–f’’, g’ Bilateral view showing internal anatomy. g, g’ Dashed lines demarcate the boundary between the head and the trunk. l1–l4 leg 1–leg 4