Fig. 6

In situ hybridization results for Wnt genes in stage 11 H. exemplaris embryos. Green color represents gene expression. Nuclei are labeled with DAPI (blue). Images that share the same letter represent data from the same embryo. Arrowheads point to internal cells that connect to the ectoderm at the posterior end. Anterior is towards the top. a Six3 expression in early elongation stage embryos. b Six3 expression in late elongation stage embryos. c Wnt2 expression. d Wnt 4 expression. e, f Wnt5 expression in early elongation stage embryos. g Wnt5 expression in late elongation stage embryos. h Wnt11 expression. i Wnt16B expression. j WntA expression. f, g’, h’’’, j’ Bilateral view showing internal anatomy. h’’ Bilateral view of outer ectoderm. All other panels show lateral views of embryos that are facing right. a’–d’, h’, i’ show internal anatomy of laterally viewed embryos