Table 1 Details on gene amplifications: primers’ name, sequence and references, and PCR conditions used
Gene | Primer | Primer sequence | References | PCR conditions (°C(time) × number of cycles) |
|---|---|---|---|---|
cyt-b | CB1 | 5′ CCATCCAACATCTCAGCATGATGAAA 3′ | [28] | 94° (3′), [94° (30″), 50° (45″), 72° (2′) × 35], 72° (10′) |
CB2 | 5′ CCCTCAGAATGATATTTGTCCTCA 3′ | [28] | ||
MVZ 16 | 5′ AAATAGGAAGTATCACTCTGGTTT 3′ | [29] | ||
12S | 12Sa | 5′ CTGGGATTAGATACCCCACTAT 3′ | [30] | 94° (3′), [94° (30″), 49° (30″), 72° (1′) × 35], 72° (5′) |
12Sb | 5′ GAGGGTGACGGGGCGGTGTGT 3′ | [30] | ||
MC1R | MC1R F | 5′ GGCNGCCATYGTCAAGAACCGGAACC 3′ | [31] | 94° (3′), [94° (30″), 50° (30″), 72° (1′) × 35], 72° (5′) |
MC1R R | 5′ CTCCGRAAGGCRTAGATGATGGGGTCCAC 3′ | |||
BDNF | BDNF_DRV_F | 5′ ACCATCCTTTTCCTKACTATGG 3′ | [32] | 94° (3′), [94º(30″), 50° (45″), 72° (2′) × 35], 72° (10′) |
BDNF_DRV_R | 5′ CTATCTTCCCCTTTTAATGGTC 3′ |