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Subject characteristics
|
n = 10 rehabilitated, adult hedgehogs
|
n = 15 rehabilitated, juvenile hedgehogs
|
n = 8 wild hedgehogs (3 juveniles, 5 unidentified)
|
| | |
n = 10 wild, juvenile hedgehogs
| |
|
Glucocorticoid analyses
| | | |
|
Sampling
|
Faecal samples (n = 10)
|
Saliva samples (n = 57)
|
Saliva samples (n = 4)
|
| | |
Faecal samples (n = 67)
|
Faecal samples (n = 9)
|
| | |
Sampling before (wild) and during captivity (both groups) and post release (both groups)
| |
|
Laboratory procedure
|
Testing:
|
Testing:
|
Testing:
|
| |
Faecal corticosterone metabolite levels (n = 10)
|
Faecal corticosterone metabolite levels (n = 67)
|
Faecal corticosterone metabolite levels (n = 9)
|
| | |
Saliva corticosterone levels (n = 57)
|
Faecal cortisol metabolite levels (n = 7)
|
| | | |
Saliva cortisol levels (n = 4)
|
|
Personality testing
|
Not performed
|
Novel arena test on day 1 in enclosures
|
Not performed
|
| | |
Novel object tests on day 3 and 5 in enclosures
| |
|
Release into the wild
|
Not performed
|
After 7 days in enclosures (n = 18, 8 rehabilitated and 10 wild juveniles)
|
Already free-living
|
|
Post release monitoring of survival and spatial behaviour
|
Not performed
|
Radio tracking of 18 juveniles, out of which 1 was unaccounted for
|
Results not included in the present study: Radio tracking of the 3 radio tagged wild juveniles (Rasmussen et al. (2019))
|
|
Data analyses
| | | |
|
Faecal corticosterone metabolite levels in relation to background, sex and health
|
Linear mixed effects model (LME) for all cohorts
|
Linear mixed effects model (LME) for all cohorts
|
Linear mixed effects model (LME) for all cohorts
|
|
Saliva corticosterone levels in relation to background, sex and health
| |
Linear mixed effects model (LME)
| |
|
Personality (novel arena and novel object tests)
| |
Principal component analyses (PCA)
| |
|
Effects of personality (shy/bold) and background (wild/rehabilitated) on post release survival
| |
Fisher's exact test
| |
