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Table 1 A flow chart presenting the research setup for the three cohorts studied

From: An exploratory investigation of glucocorticoids, personality and survival rates in wild and rehabilitated hedgehogs (Erinaceus europaeus) in Denmark

Categories Cohort 1 Cohort 2 Cohort 3
Subject characteristics n = 10 rehabilitated, adult hedgehogs n = 15 rehabilitated, juvenile hedgehogs n = 8 wild hedgehogs (3 juveniles, 5 unidentified)
   n = 10 wild, juvenile hedgehogs  
Glucocorticoid analyses    
Sampling Faecal samples (n = 10) Saliva samples (n = 57) Saliva samples (n = 4)
   Faecal samples (n = 67) Faecal samples (n = 9)
   Sampling before (wild) and during captivity (both groups) and post release (both groups)  
Laboratory procedure Testing: Testing: Testing:
  Faecal corticosterone metabolite levels (n = 10) Faecal corticosterone metabolite levels (n = 67) Faecal corticosterone metabolite levels (n = 9)
   Saliva corticosterone levels (n = 57) Faecal cortisol metabolite levels (n = 7)
    Saliva cortisol levels (n = 4)
Personality testing Not performed Novel arena test on day 1 in enclosures Not performed
   Novel object tests on day 3 and 5 in enclosures  
Release into the wild Not performed After 7 days in enclosures (n = 18, 8 rehabilitated and 10 wild juveniles) Already free-living
Post release monitoring of survival and spatial behaviour Not performed Radio tracking of 18 juveniles, out of which 1 was unaccounted for Results not included in the present study: Radio tracking of the 3 radio tagged wild juveniles (Rasmussen et al. (2019))
Data analyses    
 Faecal corticosterone metabolite levels in relation to background, sex and health Linear mixed effects model (LME) for all cohorts Linear mixed effects model (LME) for all cohorts Linear mixed effects model (LME) for all cohorts
 Saliva corticosterone levels in relation to background, sex and health   Linear mixed effects model (LME)  
 Personality (novel arena and novel object tests)   Principal component analyses (PCA)  
 Effects of personality (shy/bold) and background (wild/rehabilitated) on post release survival   Fisher's exact test