Table 3 Grid setup and environmental forcing in WT evolution and serial transfer protocol (STP)
Option (WT evolution) | Description | Value or range |
|---|---|---|
Maximum population size | As defined by the size of the grid (40x40) | 4900 |
Sub-grids | The grid is sub-divided into n grids where fluctuations are independent | 4 |
Fluctuation frequency | Probability (per time step) of 1 metabolite (A or C) changes in influx in one of the sub-grids | 0.01 |
Fluctuation range | New influx of metabolite is sampled uniformly from range | [10e-5, 10e-1] |
Extracellular metabolite outflux | Rate at which metabolites outside of cells wash out | 0.01 |
Option (serial transfer protocol) | Description | Value or range |
Maximum population size | As defined by the size of the grid (70x70) | 4900 |
Number of cells serially transferred | A (near) tenfold dilution of cells | 500 |
Time steps of cycle | This represents, for example, the“ 24 h” serial transfer protocol of the LTEE | 50 (AUT) |
[A] at beginning of cycle | Amount of resource A given at the beginning of the cycle | 1.25 |
[C] at beginning of cycle | Am mount of resource C given at the beginning of the cycle | 1.25 |
Extracellular metabolite outflux | Assuming metabolites can no longer wash out of the system | 0.0 |