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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Lacking catalase, a protistan parasite draws on its photosynthetic ancestry to complete an antioxidant repertoire with ascorbate peroxidase

Fig. 2

PAGE analysis of APX activity and protein during the purification of APX activity. Peak APX fractions were separated by nondenaturing 10% PAGE. a Gel stained with Coomassie blue. b APX activity was visualized by a nitroblue tetrazolium stain. Lanes 1: Peak APX fraction from DEAE sephacel (30 μg protein). Lanes 2: Peak APX fraction from phenyl Sepharose, Lanes 3: The fraction following the APX peak activity from phenyl Sepharose. c SDS-PAGE analysis of peak APX fraction. d Sequences of tryptic peptides identified by Edman degradation from the 32 kDa band in (c)

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BMC Ecology and Evolution

ISSN: 2730-7182

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