Table 1 List of primers used for gene expression analysis by quantitative real-time PCR (qPCR)
Gene name | Primer sequence (5’→3’) | Amplicon (bp) | Ta (°C) | Efficiency | R2 |
|---|---|---|---|---|---|
ogn1 | F: GAAGTCTCTCTTATTCACCTGT | 138 | 60 | 92.4 | 0.99 |
R: CAAAGGGTCACTGAAGTATCCA | |||||
ogn2 | F: TGTTATTCTCCCATGGATCCTG | 125 | 60 | 100 | 0.99 |
R: GATCCCCCGCTGCATCTGTGG | |||||
ogn1 | F: GAAGTCTCTCTTATTCACCTGT | 544 | 60 | na | na |
R: GTTGTTGGCATTGAAGGAT | |||||
ogn2 | F: ATGATGCAACTGAGGACTTTAA | 392 | 60 | na | na |
R: GCTCCATCTTCAATCTCAG | |||||
op | F: AAAACCCAGGAGATAAACTCAAGACAACCCA | 153 | 68 | 95.3 | 0.99 |
R: AGAACCGTGGCAAAGAGCAGAACGAA |