Fig. 1
From: Alternative splicing after gene duplication drives CEACAM1-paralog diversification in the horse
Comparison of equine CEACAM TM domains. Phylogenetic trees were constructed from transmembrane domain exon nucleotide sequences (a), and amino acid sequences (b) using the UPGMA (a) and ML (b) method (MEGA 6.0 software). The reliability of a phylogenetic tree was assessed using the Bootstrap test applying 500 replicates. The statistical support for selected nodes is shown. Boxes group CEACAMs with the indicated properties. Note that on amino acid level the association of the TM with cytoplasmic signaling motifs is not visible. The transmembrane domain of Equine CEACAM19 was used as an out group. c Amino acid sequences of transmembrane domains of equine CEACAM-1 related CEACAMs. Predicted sequence of transmembrane helices by the TMHMM Server v. 2.0 http://www.cbs.dtu.dk/services/TMHMM-2.0/ are underlined. Stop codons within the transmembrane domain exon are indicated in red. Amino acids are depicted in single letter code. TM, transmembrane domain; CC, CEACAM; ITAM, immunoreceptor tyrosine-based activation motif; ITIM, immunoreceptor tyrosine-based inhibition motif