Fig. 4

Predicted O-glycosylation patterns of stylar AGPs and NtPRP proteins among Nicotiana tabacum genotypes and Nicotiana spp. Proteins are aligned to the approximate position of the Ole e 1 domain marked by black vertical lines. The species were ordered based on the most parsimonious tree [8] with the exception of N. tomentosiformis, which was placed adjacent to N. tabacum to allow better comparison of this species to its ancestral version of this gene in N. tabacum. The same color represents the same glycosylation site. A different color shade shows amino acid polymorphism found among species within the same O-glycosylation site. Colors match the same site only within each gene, not among genes. * - designated species with most distinct O-glycosylation pattern among Nicotiana spp.