Fig. 7
From: Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor
Cross-species interaction studies. Coomassie Blue-stained SDS-PAGE gels and corresponding chromatograms of analytical gel filtration experiments. a ScPrp38 binds hsMFAP1267-344, a minimal hsPrp38-interacting fragment. b HsPrp38NTD+, but not hsPrp38NTD+,D145A, binds scSpp381. All experiments were performed with a Superdex 75 10/300 column (GE Healthcare). M – marker; L – load; horizontal black lines – fractions analyzed by SDS-PAGE