Fig. 9

Expression of War-Paxβ. Anterior faces up in all panels. Dorsal (d)–ventral (v) left (l)–right (r), and anterior (a)–posterior (p) axes indicate the orientation. First and second column are 3D rendered images based on autofluorescence (turquoise) and reflection signal of War-Paxβ expression staining (yellow). The ventral half of the larvae in the first column was omitted in order to visualize the location of the ventral gene expression signal. The lateral left larval hemisphere in images of the second column was omitted in order to enable a lateral view of the gene expression signal. Clipping plane projections of the respective panels are indicated by dotted white lines. Third and fourth column are light micrographs. Location of the prototroch is indicated by dashed black lines and the location of the mouth opening is indicated by an asterisk. Numbers mark distinguishable expression domains. a Ventral view. b Lateral left view. c Ventral view. d Lateral left view. e Ventral view. Note the first expression signal in cells of the developing cerebral ganglia (#1). f Lateral left view. g Ventral view. h Lateral left view. I Ventral view. Note the expression in the neuroectoderm of the developing ventral nerve cords (#2) and cerebral ganglia (#1). j Lateral left view. k Ventral view. l Lateral left view. m Ventral view. Note War-Paxβ expression in cells of the cerebral ganglia (#1) and the faint expression in the region of the developing ventral nerve cords (#2) as well as the expression in cells flanking the mouth opening at the base of the invagination of the peri-imaginal space (#4). n Lateral left view. o Ventral view of the most ventral part of a late larva. p Ventral view. Note the weak War-Paxβ expression in the CNS (#1, #2). Abbreviation: foregut (fg). Scale bar: 50 μm