Fig. 6

Quantitative real-time PCR (qPCR) expression levels (shown as Fold Change, RQ) between biological groups (putative venom glands (pvg), pharyngeal lobes, and posterior body wall) of five analyzed GLTx transcripts (GLTx paralog 1–3, and adjacent gene regions GLTx-3’ and GLTx-5’; for details see Material and methods section “Quantitative real-time PCR”) in G. tridactyla (n = 10; ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05). a Relative GLTx expression (logarithmic scale) in putative venom glands (grey) and pharyngeal lobes (orange) in comparison to the GLTx expression signal exhibited by the body tissue (RQ = 1). Relative GLTx expression in the pharyngeal lobes and putative venom glands is significantly different from the expression signal in the body tissue. b Relative GLTx expression (linear scale) within the pharyngeal lobes in comparison to the putative venom glands (RQ = 1). Relative GLTx expression is significantly different between both putative venom glands and pharyngeal lobes