Fig. 3

Responses of ancestral TAS2R1 and TAS2R4 receptors. a Responses of ancestral TAS2R1s to camphor. TAS2R1s of ancestors 1, 2, 3, and 4 (Fig. 2a) were expressed in HEK293T cells and their responses to camphor were measured using calcium assays. b Response amplitudes of ancestral TAS2R1s to 0.1 mM camphor. **p < 0.01,†p < 0.10, two-sided Welch’s t-test). Any of the node pairs in Fig. 2a do not show significant differences (p > 0.10). c Responses of ancestral TAS2R4 to colchicine. TAS2R4s of ancestors 1(4), 2, and 3 (Fig. 2b) were expressed in HEK293T cells and their responses to colchicine were measured using calcium assays. Because ancestors 1 and 4 have the same sequence, they were grouped together in Fig. 3c and d. d Response amplitudes of ancestral TAS2R4s to 0.1 mM colchicine. ***p < 0.001, **p < 0.01, two-sided Welch’s t-test). Any of blanches in Fig. 2b do not show significant differences between the nodes (p > 0.10)