Adaptive patterns in the p53 protein sequence of the hypoxia- and cancer-tolerant blind mole rat Spalax

Table 1 Domains with different intra-group similarities for hypoxia-tolerant (HT) and hypoxia-sensitive (HS) taxa

	Evolutionary tree	TAD1	TAD2	PRD	DBD	NLS	TDRD
U _obs	35	49	12	74	9	50	3
p _MW	0.2791	0.4844	0.0015	0.0313	0.0005	0.4861	0.0001
p _MW -FDR		0.4861	0.0029	0.0468	0.0015	0.4861	0.0003
p _MC	0.413	0.4895	0.0095	0.0768	0.0046	0.4882	0.0005
p _MC -FDR		0.4895	0.019	0.1152	0.0140	0.4895	0.0030

Statistics U and p-values were calculated for domain sequences of p53 and for evolutionary tree positions (see Methods). P _MW for p53 domains corresponds to p-value calculated in a standard one-tailed Mann–Whitney test [46]. P _MC correspond to p-values calculated in our extension on U test using Monte-Carlo simulations (see Methods). As can be seen it the table, the estimated ‘divergence time’ calculated based on evolutionary tree is unlikely to explain differences between the HT and HS groups, seen in the TAD2, DBD, and TDRD domains

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