Fig. 3
From: ERK1 and ERK2 present functional redundancy in tetrapods despite higher evolution rate of ERK1
ERK2 is not detected in snakes and lizards. a, b Western-blot analysis performed as in Fig. 1 incubated with a polyclonal anti-ERK antibody (upper panels) or anti-phosphoERK antibody (lower panels). a brain extracts from snakes, lizards, crocodile and turtles (full taxonomic names in Additional file 7). Control brain extracts from cattle. CO: cortex, CE: cerebellum, ME: medulla, OL: olfactory bulb, PI: pineal gland, W: whole brain. Note that degraded bands are observed in turtle E. orbicularis likely due postmortem sampling. b extracts from lizard embryo fibroblasts were stimulated or not (NS) one hour in presence of FCS alone (FCS) or FCS + phosphatase inhibitor NaVO4—(FCS + NaVO4—) to activate ERKs