Lipids containing medium-chain fatty acids are specific to post-whole genome duplication Saccharomycotina yeasts

Table 2 Total fatty acid content in the wild-type (WT), tgl3Δ and loa1Δ strains grown in various conditions

Strain	Media	Temperature (°C)	Fatty acid (μg FAME.mg⁻¹ dw)	Relative to WT	28 °C vs 23 °C	Relative to YP 28 °C	Relative to YP 23 °C	High N vs low N
WT	YP	23	54.92 ± 4.62		+22 %*
WT	YP	28	45.05 ± 1.87
WT	High-N YNB	23	56.13 ± 6.03		+7 %		+2 %
WT	High-N YNB	28	52.38 ± 5.29			+16 %*
WT	Low-N YNB	23	62.19 ± 2.16		+10 %*		+13 %	+11 %
WT	Low-N YNB	28	56.39 ± 2.73			+25 %***		+8 %
tgl3Δ	YP	23	69.65 ± 4.19	+27 %*	+27 %***
tgl3Δ	YP	28	54.70 ± 1.22	+21 %**
tgl3Δ	High-N YNB	23	71.59 ± 10.01	+27 %	+24 %		+3 %
tgl3Δ	High-N YNB	28	57.71 ± 6.4	+10 %		+5 %
tgl3Δ	Low-N YNB	23	77.54 ± 2.17	+25 %***	+10 %***		+11 %**	+8 %
tgl3Δ	Low-N YNB	28	70.13 ± 1.94	+25 %**		+28 %***		+22 %*
loa1Δ	YP	23	60.73 ± 1.73	+10 %	+23 %***
loa1Δ	YP	28	49.21 ± 0.63	+9 %*
loa1Δ	High-N YNB	23	52.81 ± 7.86	−6 %	nd		−13 %*
loa1Δ	High-N YNB	28	nd			nd
loa1Δ	Low-N YNB	23	70.56 ± 4.06	+13 %*	+27 %***		+16 %*	+34 %**
loa1Δ	Low-N YNB	28	55.44 ± 4.73	−2 %		+11 %*		nd

Data are expressed as the mean ± SE (n = 3). Significant differences according to Student’s t-test
***P < 0.001
**P < 0.01
*P < 0.05

ISSN: 2730-7182