Phylogeography of the land snail genus Orcula(Orculidae, Stylommatophora) with emphasis on the Eastern Alpine taxa: speciation, hybridization and morphological variation

Table 2 Primer sets

Region	Primer (5′ to 3′)	Origin	Fragment size	T°C RocheTaq
*COI* fwd	COIfolmerFw: GGTCAACAATCATAAAGATATTGG	[54]	655 bp	50°C
*COI* rev	H2198-Alb: TATACTTCAGGATGACCAAAAAATC	[55]
*12S* fwd	12SGastFw: TTACCTTTTGCATAATGGTTAAACTA	[56]	669 - 725 bp	54°C
*12S* rev	12SGastRv: CGGTCTGAACTCAGATCATG	[56]
*16S* fwd	16SLOrc1_fwd: TTACCTTTTGCATAATGGTTAAACTA	[19]	838 - 890 bp	54°C
	16SLOrc2_fwd: TTACCTTTTGCATAATGGTTAAATTA	[19]
*16S* rev	16SLOrc_rev: CGGTCTGAACTCAGATCATG	[19]
H4 / H3 fwd	OrcH4_left1: GTGCGTCCCTGGCGCTTCA	[19]	848 - 1095 bp	57°C
	OrcH4_left2: GGCGCTTCAGGGCGTACACT	[19]
H4 / H3 rev	OrcH3_right1: TGGGCATGATGGTGACACGCT	[56]		Finnzymes
H4 / H3 rev	OrcH3_right1: TGGGCATGATGGTGACACGCT	[56]		Phusion: 71°C
intern fwd	OrcH4S_left3: CGGTCTGAACTCAGATCATG	[19]
intern rev	OrcH3S_right3: CGGTCTGAACTCAGATCATG	[19]

Primer sequences for amplification and sequencing of the COI, 12S, 16S and H4/H3 fragments. The H4/H3 fragments were amplified with Finnzymes Phusion polymerase, wherefore the respective annealing temperatures are provided in addition.

ISSN: 2730-7182