Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes

Table 2 Specific activity of enzymes examined with peptides designed from N-terminal regions

	Sequence of substrate	Species	Specific activities (μmol • mg enzyme^–1 • 30 min^–1)
			MfHE1	MHCE	ZHE1	MfHE3	MLCE
1	NPQVPQ↓YPSKPQ	Medaka	1.55	9.76	1.01	0.02	0.05
2	NPSYPQ↓NPSYPQ	Medaka	4.87	16.1	2.11	0.002	0.08
3	PPSKPQ↓YPNPQT	Killifish	ND	0.66	ND	0.018	ND
4	QPQTPS↓YPQQPQ	Killifish	1.05	2.46	0.42	0.039	0.57
5	PLPVR↓VEEVV	Zebrafish	ND	0.02	0.44	ND	ND
6	KLMLQ↓APEPF	Zebraish	1.87	3.36	1.85	1.28	2.33
7	TVQQS↓DYLIK	Zebrafish	2.93	2.74	3.06	1.19	1.00
8	FQQQS↓APQRY	Milkfish	8.07	1.36	2.68	0.28	3.52
9	PLPLK↓AEPFQ	Milkfish	4.27	2.30	6.38	0.79	ND
10	KQVLQ↓GPVKE	Milkfish	0.57	ND	0.20	0.03	ND

Arrows indicate cleavage sites. The numbers on the left correspond to the substrate numbers in Figure 8.

ISSN: 2730-7182