Phylogeny and distribution of Wolbachia infections among haplotypes. The left part of the figure shows the phylogeny of the 10 H. bolina mtDNA haplotypes with respect to H. alimena, H. octocula, H. antilope, H. pandarus, H. missipus, H. anthedon, H. usambara, with Precis tugela as an outgroup. The tree was constructed via Maximum Likelihood estimation in Treefinder; aLRT (approximate likelihood ratio test) scores are given next to the nodes (only for internal nodes for clarity) and branch length indicates the number of substitution per site. H. bolina haplotypes are in italics. Genbank accession numbers are given in parenthesis. The right part of the figure gives the distribution of the various Wolbachia infections status (u: uninfected, wBol1a, wBol1b, wBol2) observed with each haplotype. Note the relative proportions of the infections are not representative of the prevalence within the species since sampling was aimed at representing the different infection status in even proportions.