Multicolor fluorescent in situ hybridization images of O. officinalis complex. Hybridized by C genome-specific probes (red), together with B genome probes (green, e-g, n-s) or E genome probes (green, h-m, t-v), counterstained by DAPI (blue, a-d, k-m). Arrows indicated inter-genomic (B-C genomes) translocations, which were enlarged in the below box (e-g). (a) O. officinalis (CC), and its karyotype (b). (c) O. eichingeri (CC), and its karyotype (d). (e-f) O. punctata (BBCC). (g) O. malampuzhaensis (BBCC). (h, k) O. latifolia (CCDD). (i, l) O. grandiglumis (CCDD). (j, m) O. alta (CCDD). Parental genomes in the nuclei of allotetraploids, separated spatially in O. punctata (n, interphase; o, prophase; p, anaphase), in O. malampuzhaensis (q and r, interphase stages; s, anaphase), and in interphase nuclei of O. latifolia (t), O. grandiglumis (u), and O. alta (v), respectively. Bar, 5 μm.