Invertebrate laforin orthologs bind amylopectin. Recombinant proteins were incubated with 5 mg/ml amylopectin, amylopectin was pelleted by ultracentrifugation, and proteins in the pellet (P) and supernatant (S) were visualized by Western analysis. VHR, H. sapiens VHR; Hs, Hs-laforin; Bf, Bf-laforin; Nv, Nv-laforin. Mutated residues are marked with an asterisk in Fig. 3.