Molecular evolution of dimeric α-amylase inhibitor genes in wild emmer wheat and its ecological association

Table 2 Specific primers designed from SNPs in the dimeric α-amylase inhibitor genes

Primer	Sequences*	Fragment size	Temperature	Cycle
W19G	ATGCTCGTGGCGACACT CG	426	64	32
W24A	TCGTGGCGACACCCRTAC CA	422	63	35
W35A	ACCCATAGCAGCCCAGTACAA	412	62	35
W46A	CGAGTACGACGCATGGAT CA	400	65	35
W47AT	AGTACGACGCATGGAGT AT	398	57	35
W125G	CTGTCGTCCATTGCTT AG	319	62.5	35
W127G	CTGTCGTCCATTGCTGAGGG	319	62.5	35
W190C	CTGCTGCCAGCAGCTCGG CC	256	57	35
W195T	TGCCAGCAGCTCGCCGACATT	252	51	35
W207T	ACATCAGCGAGTGGA GT	236	60.5	30
W259A	CATGTATAAGGAGCATGT CA	187	61	35
W263C	TATAAGGAGCATGGCGTC TC	183	62	30
W263A	TATAAGGAGCATGGCGTC TA	183	65	35
W276A	GCGTGTCGGAGGGACAGT CA	170	60	35
W288CG	GACAGGCRGGGACAGGA A CG	158	66	30
W288AG	GACAGGCGGGGACAGGA A AG	158	63.5	30

*SNP positions were on the 3' end of the primers and are identified in bold letters. Extra mismatched nucleotides were also incorporated in the primers (underlined).

ISSN: 2730-7182