Table 4 Amino acid changes associated with the divergence of human bestrophins

in vertebrate Best1

in vertebrate Best4

264

264: Y, conserved

279: P, Q, S, L, E, G or Y

Loop 3

265*

265: P, conserved

280: A, T, Q, E or K

Loop 3

266

266: G, conserved

281: L, A, V, P, K, E or D

Loop 3

267

267: H, conserved

282: G, W, P or H

Loop 3

331*

331: R, P, V, I, L or M

346: P, conserved

C-terminus

343

343: P, conserved

358: P, A, F or V

C-terminus

349

349: A, conserved

364: V, I, L, P, Q or A

C-terminus

in vertebrate Best2

in vertebrate Best4

45*

45: A, conserved

45: V, L, M, F or T

TMD2

52*

52: F, conserved

52: L, K, R, C or F

Loop 1

59

59: K, conserved

59: R, K or Q

Loop 1

61*

61: Y, conserved

61: V, I, L, M or E

Loop 1

66

66: V, A or S

66: A, conserved

Loop 1

91

91: N or H

91: N, conserved

Loop 2

106

106: L or V

106: L, conserved

Loop 2

132

132: A, S or C

132: A, conserved

Loop 2

206

206: A, T or S

206: A, conserved

Loop 2

207*

207: L, F or Y

207: L, conserved

Loop 2

263*

263: G, conserved

263: G, E, A or P

Loop 3

264

264: Y, conserved

279: P, E, Q, G, S or Y

Loop 3

331

331: V, M or P

346: P, conserved

C-terminus

349

349: A, conserved

364: V, P, Q, L or A

C-terminus

in vertebrate Best3

in vertebrate Best4

60*

60: R, conserved

60: Y, H, R, S or D

Loop 1

115

115: H, Q, R or L

115: H, conserved

Loop 2

209*

209: S, T, M or N

209: L, conserved

Loop 2

264

264: Y, conserved

279: P, Q, S, L, E, G or Y

Loop 3

266

266: G, conserved

281: L, V, E, P, K, D or A

Loop 3

331

331: K or R

346: P, conserved

C-terminus

in vertebrate Best2

in vertebrate Best3

165*

165: R, conserved

165: T, A, P, S or R

Loop 2

268

268: D, T, S or N

268: D, conserved

Loop 3

333

333: E, conserved

333: K, R, E, A, N or T

C-terminus

354*

354: Q, L, D or V

354: Y, conserved

C-terminus, AI domain

365

366: D, conserved

365: Q, D, E or H

C-terminus, AI domain