Phylogenetic tree of 53 homologues of vertebrate bestrophins. The phylogenetic tree of the bestrophin protein family was inferred by the neighbor-joining method (NJ) method applying gamma corrected distances. Urochordata (Ciona intestinalis [Ci] and Ciona savigny [Cs]) were used as out-group. The support for each phylogenetic group was tested with 1000 bootstrap pseudoreplicates. Protein sequences are given in Table 1.