Figure 5
Serine protease inhibitors in A. labialis venom gland. A) Alignment of Kunitz-type serine protease inhibitors. â– , conserved six cysteine residues in kunitz domain. Residues that are different from the consensus sequence of A. labialis proteins are highlighted. The number of clones is shown in italics and the predicted signal peptide using SignalP 3.0 is underlined. B) Alignment of clone 655 (serine protease inhibitor containing two Kunitz domains) with bikunin [29]. Signal peptide of clone 655 is indicated by a vertical bar ( | ), â– , conserved six cysteine residues; 
, N-glycosylation site. Conserved residues are highlighted. C) Alignment of isoforms of two Kunitz-type domains. The presence of two Kunitz-type domains was further confirmed by PCR using gene specific primer and reverse primer from cDNA construction kit (for details, see Materials and methods). *, consensus residues and residues that are different from the consensus sequence are highlighted. The number of clones is shown in italics and the predicted signal peptide using SignalP 3.0 is underlined.