Figure 2

Phylogenetic analysis of the intron-exon boundaries of the PMCHL genes.(A) Comparative sequence analysis of PMCHL splice donor and acceptor sites. Exonic nucleotides are in uppercase letters, and intronic nucleotides are in lowercase. Consensus splice donor and acceptor sequences are indicated. The most invariant dinucleotides gt and ag are in bold characters. Sequence differences in these dinucleotides are boxed in grey. Dashes indicate identity with the sequence in the first row. Brackets indicate a gap. GenBank accession numbers and positions in http://www.ensembl.org are as follows: PMCH HSA [GenBank:NM002674], PMCHL1 HSA chr 5p14, 22178218–22188421, PMCHL2 HSA chr 5q13, 70707368–70717576, PMCHL1 PTR chr 5, 93832488–93866206, PMCHL2 PTR chr 5, 44567880–44600471, PMCHL PPA [GenBank:EF043264], PMCHL PPY [GenBank:AY008415, GenBank:AY008422, GenBank:AY008419], PMCHL HLA [GenBank:EF043266], PMCHL CAE [GenBank:EF043268], PMCHL MML chr 6, 22205153–22215832, PMCHL MFA [GenBank:EF043267], PMCHL CHA [GenBank:AY008430], PMCHL SOE [GenBank:EF043262], PMCHL CAP [GenBank:EF043263], PMCHL CCA [GenBank:AY008431]. (B) Schematic representation of the PMCHL1 and PMCHL2 exon/intron structures in Homo sapiens (HSA), Pan troglodytes (PTR), Macaca mulatta (MML) and Macaca fascicularis (MFA). The position of the retroposed sequence derived from the antisense strand of the PMCH locus is indicated and detailed correspondence between the sequences is given (inset). Times of divergence are indicated (Mya).