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Table 1 Oligonucleotide primers used for desaturase gene characterization and functional expression

From: Key biosynthetic gene subfamily recruited for pheromone production prior to the extensive radiation of Lepidoptera

Degenerated Primer sequencea
PR3 5'-GGYATYACVGCHGGNGCWCA-3'
PR4 5'-TGRTARTTRTGGAABSCYTCNCC-3'
Gene specific b Primer sequence
Lc1–5a 5'-CAGGCCAGCGGCATCAGCAATAAGTA-3'
Lc1–5b 5'-CGAGGATTAATCTCAGCGGCCACT-3'
Lc1–3a 5'-TACTTATTGCTGATGCCGCTGGCCTGC-3'
Lc1–3b 5'-TCCTGGAAAAACGCGCTCTTCGTAGCTGCAATG-3'
Lc2–5a 5'-TCCTTTGGCCTTGATTTCGGGGTGTTTC-3'
Lc2–5b 5'-CCATGAGCCAACCCATGTGGGAGAAGAA-3'
Lc2–3a 5'-GGTGAAGAAACACCCCGAAATCAAGGCC-3'
Lc3–5a 5'-TCGCTCATGTCAATGCTGGCTCCCTTCT-3'
Lc3–5b 5'-ACGTTTGGCGTTGTGAGGATCAGCGTC-3'
Lc3–3a 5'-TTGAGGCGGACCCCATCGTCATGTTT-3'
Lc3–3b 5'-TGGTACGTGGCTACAATCCTGCGGTTCA-3'
Lc4–5a 5'-GTCAACAGCCAACCGACATGCGAA-3'
Lc4–5b 5'-GAGTCGGGATAACGAAGCAAACCA-3'
Lc4–3a 5'-TACGCAATTCCCTTCGTCGGATTG-3'
Lc4–3b 5'-CTTATGCCGATGTACTTCTGGAACG-3'
Full-length amplification b Primer sequence
Lc1-fls 5'-GATTCATAGATTCGTGTTCGGTGA-3'
Lc1-flas 5'-CAGGGACCTCGAAGTGACCTTT-3'
Lc2-fls 5'-GCAGTGATTGGTGTCGTGCGGA-3'
Lc2-flas 5'-AACAATAAAATATTTATTTACATTAATTTGG-3'
Lc3-fls 5'-TAAAGCAAGTTACTGTCGCCG-3'
Lc3-flas 5'-GAAATACGTTTATTATTGGAATAAATAA-3'
Lc4-fls 5'-GAGTCACAAGTTGTTCAGTCGTAA-3'
Lc4-flas 5'-TCCATAAGATGGGTACGTATATTGT-3'
Expression YEpOLEX Primer sequence c
Lc1ORFs 5'-gctctagaATGCCGCCGAACGTGACAG-3'
Lc1ORFas 5'-cgagctcgCTAATCATCTTTACGGTTAATG-3'
Lc2ORFs 5'-gctctagaATGCCGCCCGAGGGTCTAAT-3'
Lc2ORFas 5'-cgagctcgTCAAAAAGATTTCTCCGGAT-3'
Lc3ORFs 5'-gctctagaATGGCTCCAAACATCCTGGG-3'
Lc3ORFas 5'-cgagctcgTCACTGTTCTTTAGGATGTGC-3'
Expression pYEX Primer sequence c
Lc4ORFs 5'-ggatccATAATGGCGCCATATCCTGAAG-3'
Lc4ORFas 5'-gaattcCGATTTCATATTAGTTCATTTTAATC-3'
  1. a [21]; Y = C or T; V = A, G or C; H = A, C or T; N = A, G, C or T; W = A or T; R = A or G; B = G, T or C; S = G or C.
  2. b Lc1= Lca-QPAQ, Lc2 = Lca-SPVE, Lc3 = Lca-GATD, Lc4= Lca-KPVQ
  3. c Restriction sites are indicated in lower case letters, start and stop codons are marked in bold.