Figure 1

Amino acid alignment of catalytic site motifs and gene architecture of LACTB orthologs. (A) Schematic organization and alignment of the three PBP-βLs catalytic site motifs (highlighted in green) and their flanking regions in LACTB orthologs. The corresponding motifs in the Streptomyces sp. strain R61 D-alanyl-D-alanine carboxypeptidase [Swiss-Prot:P15555] are included. Abbreviations for species names, in order: Homsa, Homo sapiens (Swiss-Prot:P83111); Macmu, Macaca mulatta (Ensembl:ENSMMUP00000004719); Musmu, Mus musculus (Swiss-Prot:Q9EP89); Orycu, Oryctolagus cuniculus (Ensembl:ENSOCUP00000008608); Canfa, Canis familiaris (RefSeq:XP_544713); Bosta, Bos taurus (Swiss-Prot:P83095); Mondo, Monodelphis domestica (Ensembl:ENSMODP00000013893); Galga,Gallus gallus (Swiss-Prot:Q5ZK12); Xentr, Xenopus tropicalis (Ensembl:ENSXETG00000009720); Fugru, Fugu rubipes (Ensembl:SINFRUP00000138119); Oryla, Oryzia latipes (Ensembl:ENSORLP00000009787); Gasac, Gasterosteus aculeatus (Ensembl:ENSGACP00000014046); Danre, Danio rerio (RefSeq:NP_001018429); Strpu, Strongylocentrotus purpuratus (RefSeq:XP_789736); Cioin, Ciona intestinalis (Ensembl:ENSCING00000006798); Schja, Schistosoma japonicum (GenPept:AAX27853, AAX25200); Caeel, Caenorhabditis elegans (RefSeq:NP_001041033); Caebr, Caenorhabiditis briggsae (GenPept:CAE74593); Dicdi, Dictyostelium discoideum (Swiss-Prot:Q55CN2); Stesp, Streptomyces sp strain R61. The mitochondrial import sequence (Mito) is indicated. Amino acid conserved in all taxa are highlighted in yellow. (B) Organization of exons and introns in LACTB genes of representative metazoan taxa.