Tertiary structure of numit RPS13. The structures of RPS13 from E. coli (A) and T. thermophilus (B) are shown with amino acids from numit RPS13 plotted, as in the alignment (Figure 4). Red regions and plus signs indicate relative positions of positively selected sites. Green regions and asterisks indicate relative positions of amino acids in numit Rps13 in at least one rosid species that have mutated to the amino acid present in mt rps13 genes (Figure 4). Among them, sites 4 and 10 (Figure 4) are located in the coil region of the N-terminal end, site 15 is in the beginning of the first β-sheet, site 21 is in the first α-helix, site 32 is in the second α-helix, sites 42 and 43 are in the coil region between the second α-helix and the second β-sheet, site 53 are in the third α-helix, site 67 is in the coil region between the third α-helix and the fourth α-helix, sites 74, 79, and 80 are in the fourth α-helix, and sites 97, 107, 108, and 110 are in the C-terminal coil region.