Figure 5

Multiple sequence alignment of TrpB sequences. Representatives of the four groups of TrpB sequences were aligned. Ssolfa_o2C, Ptorri_o2E, and Tacido_S2E represent TrpB2_a sequences. Ptorri_i2E, Ssolfa_i2C, and Stokod_i2C represent TrpB2_i, Maceti_o2E, Afulgi_o2E, and Tmarit_o2B represent TrpB2_o sequences. Maceti_i1E, Tmarit_i1B, Afulgi_i1E, and Pfurio_i1E represent TrpB1 sequences. The 2D-structural elements of Pfurio_i1E, as deduced from the PDB file 1WDW, are shown below the sequences, and residues involved in protein interaction with TrpA (I) are assigned under 'Interface'. The line Jpred (top) lists a 2D-prediction of Ssolfa_o2C generated by using the Jpred server [37]. Residues in bold face printing are conserved; black residues are strictly, grey residues are less strictly conserved. Active site residues are plotted in italics; residues in contact with ligands are underlined. These data were deduced from the PDBsum pages [56] and the PISA server [58] of the EMBL-EBI. Residues printed in boxes were predicted by SDPpred [38] as being specific for TrpB1 or TrpB2. See legend of Figure 1 for an explanation of sequence acronyms.