Figure 3

Phylogenetic analyses of fugu, zebrafish and coelacanth protocadherin genes. Phylogenetic trees for Pcdhα (a) and Pcdhγ (b) clusters. Protein sequences of the EC1-EC3 ectodomain region were aligned by ClustalX and phylogenetic trees were built by the Neighbor-joining method based on sequence distance matrix. Numbers at the nodes are bootstrap values of 1000 replicates. Only bootstrap values above 500 at the major branches are shown. The trees are unrooted. Genes in individual Pcdhα or Pcdhγ clusters are labeled by the same color.