Figure 3

Maximum intensity z-projections of representative gorilla and chimpanzee fibroblast nuclei after 3D-FISH with BAC 39M12 (green) and a chromosome 4 painting probe (red). The chromosomal counterstain is shown in blue (scale bar = 5 μm). A) and B) gorilla nuclei (A with both alleles located at the nuclear edge, and B with one allele showing a "track" of hybridisation signals – arrow); C) chimpanzee nucleus with one 4q35.2 allele abutted against the nuclear envelope.